An effective cold storage method for stockpiling <i>Halyomorpha halys</i> eggs for field surveys and laboratory rearing of <i>Trissolcus japonicus</i>

Wong, Warren H. L.; Walz, Matt A.; Oscienny, Angela B.; Sherwood, Jade L.; Abram, Paul K.

doi:10.1101/2020.04.30.071183

Cited by 7 publications

(12 citation statements)

References 58 publications

(89 reference statements)

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“…The target host H. halys can be reared easily in the lab and egg parasitoids have been reared with success on both fresh and previously frozen egg masses (although a reduction in frozen egg suitability correlated with increased storage time has been reported) [ 23 , 24 , 25 ]. The use of short-term cold-stored egg masses of H. halys was also proposed recently [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Rearing Trissolcus japonicus and Trissolcus mitsukurii for Biological Control of Halyomorpha halys

Peverieri

Dieckhoff²,

Giovannini

et al. 2020

Insects

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Halyomorpha halys is a severe agricultural pest of Asian origin that has invaded many countries throughout the world. Pesticides are currently the favored control methods, but as a consequence of their frequent use, often disrupt Integrated Pest Management. Biological control with egg parasitoids is seen as the most promising control method over the long-term. Knowledge of the reproductive biology under laboratory conditions of the most effective candidates (Trissolcus japonicus and Trissolcus mitsukurii) for optimizing production for field releases is strongly needed. Rearing of these egg parasitoids was tested by offering three different host supply regimes using new emerged females and aged, host-deprived females in different combinations. Results showed a mean progeny per female ranging from 80 to 85 specimens for T. japonicus and from 63 to 83 for T. mitsukurii. Sex ratios were strongly female biased in all combinations and emergence rates exceeded 94% overall. Cumulative curves showed that longer parasitization periods beyond 10–14 days (under the adopted rearing regimes) will not lead to a significantly increase in progeny production. However, ageing females accumulate eggs in their ovaries that can be quickly laid if a sufficient number of host eggs are supplied, thus optimizing host resources. Our data showed that offering H. halys egg masses to host-deprived female Trissolcus once a week for three weeks allowed its eggs to accumulate in the ovary, providing the greatest number of offspring within a three week span.

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Section: Introductionmentioning

confidence: 99%

Rearing Trissolcus japonicus and Trissolcus mitsukurii for Biological Control of Halyomorpha halys

Peverieri

Dieckhoff²,

Giovannini

et al. 2020

Insects

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“…Our results showed that the storage of P. ricini eggs caused adverse effects on in the biological parameters of the F1 generation of O. pityocampae and O. kuvanae adults, represented by the rate of adult emergence, development time, longevity and fecundity. These results can be explained as follows: first, lethal effects occur during parasitoid development in low-quality stored eggs, and second parasitoids fail to accept stored eggs as hosts (Wong et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Effects of differentPhilosamia ricini(Lepidoptera: Saturniidae) cold storage periods onOoencyrtus pityocampaeandOoencyrtus kuvanae(Hymenoptera: Encyrtidae) rearing

Tunca

Cosic²,

Venard³

et al. 2022

Bull. Entomol. Res.

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Ooencyrtus pityocampae and Ooencyrtus kuvanae are egg parasitoids that are considered potential candidates for the control of different pest species through inundative release. The aim of this study was to assess the effects of different cold-storage periods of Philosamia ricini eggs (host) on the rearing parameters of O. pityocampae and O. kuvanae. Host eggs were stored at 3 °C, and a factorial experiment involving two parasitoid species, nine host storage periods (1, 5, 10, 15, 30, 45, 60, 75 and 90 days) and a control, and two host ages (1 and 2 days) was conducted, with 10 replications including 40-P. ricini eggs each. Adult emergence, development time, longevity, and fecundity were investigated. The parasitoid adult emergence percentage significantly varied with storage duration. These values were lower in O. kuvanae than in O. pityocampae. The development time of O. kuvanae progeny increased in both host age groups except in the 1-day storage period subgroup. However, the development times of the progeny of O. pityocampae reared on one-day-old eggs stored for 5, 10, 60, and 75 days were increased, and the development times of the progeny of O. pityocampae reared on 2-day-old eggs stored for 45 and 90 days were increased. The longevity of the F1 progeny of O. kuvanae was negatively affected by storage time. There was no difference in the longevity of the F1 progeny of O. pityocampae compared to that of the control. Additionally, the fecundities of the F1 progeny of O. pityocampae and O. kuvanae were 54.7 and 47.0 offspring/female, respectively. These results provide useful information for guiding the development of mass rearing methodologies for both parasitoid species.

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“…(Piperaceae) as egg‐laying substrates. The egg masses used to maintain the parasitoid colony were collected daily and underwent cold storage at 9 °C according to Wong et al (2020). Freshly laid egg masses (<24 h old) were utilized in the experiment.…”

Section: Methodsmentioning

confidence: 99%

Biocontrol implications of multiparasitism by Trissolcus mitsukurii and Trissolcus japonicus on the invasive brown marmorated stink bug

Costi

Bella

Iotti

2022

Entomologia Exp Applicata

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The egg parasitoids Trissolcus japonicus (Ashmead) and Trissolcus mitsukurii (Ashmead) (Hymenoptera: Scelionidae) are the most effective biocontrol agents of the invasive agricultural pest Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) in its native range (east Asia). Trissolcus japonicus and T. mitsukurii are sympatric in the native areas. In northern Italy, where H. halys is a major pest of fruit orchards, adventive populations of both species are spreading, and T. japonicus is artificially released under a classical biocontrol program against H. halys. This laboratory study aimed to assess the outcome of competition when both species share the same resource and possible implications for the biocontrol of the invasive stink bug. Egg masses of H. halys were offered to each parasitoid after previous parasitization by the other species. Parasitoid behaviour, number of ovipositions, and successfully developed parasitoids were recorded. Additionally, contest behaviour was assessed when both species were released simultaneously on the same egg mass. Resultsshowed that both T. japonicus and T. mitsukurii were able to parasitize an egg mass already parasitized by the other species. Competition occurred within the host eggs and each species outperformed the other when it was the first to oviposit. Importantly, the overall contribution to H. halys mortality was not affected by the interaction between parasitoids, as nonparasitized eggs were 4-6% in the absence of competition and <8% in its presence, respectively. When simultaneously released on the egg mass, T. mitsukurii was more aggressive, engaging in chase-off events in 71% of cases compared to 50% of T. japonicus.

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An effective cold storage method for stockpiling Halyomorpha halys eggs for field surveys and laboratory rearing of Trissolcus japonicus

Cited by 7 publications

References 58 publications

Rearing Trissolcus japonicus and Trissolcus mitsukurii for Biological Control of Halyomorpha halys

Rearing Trissolcus japonicus and Trissolcus mitsukurii for Biological Control of Halyomorpha halys

Effects of differentPhilosamia ricini(Lepidoptera: Saturniidae) cold storage periods onOoencyrtus pityocampaeandOoencyrtus kuvanae(Hymenoptera: Encyrtidae) rearing

Biocontrol implications of multiparasitism by Trissolcus mitsukurii and Trissolcus japonicus on the invasive brown marmorated stink bug

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