An effective assessment of valproate sodium-induced hepatotoxicity with UPLC–MS and 1HNMR-based metabonomics approach

Huo, Taoguang; Chen, Xi; Lu, Xiumei; Qu, Lei; Liu, Yang; Cai, Shuang

doi:10.1016/j.jchromb.2014.08.011

Cited by 29 publications

(35 citation statements)

References 30 publications

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“…In future studies, the concentration dependent changes of the PC/PE species in mice upon APAP treatment should be observed, which might provide valuable information for judging prognosis of DILI. Huo T et al performed both UPLC-MS and (1)HNMR-based metabonomics analysis of serum samples from 34 epileptic patients after valproate sodium treatment, and found that certain LPCs such as LPC 16:0, LPC 18:0, LPC 18:1 and LPC 18:2 decreased in valproate sodium induced liver injury [ 32 ]. The phospholipid profile of serum in human intoxicated with APAP also might be further observed by lipidomic techniques.…”

Section: Discussionmentioning

confidence: 99%

Liquid chromatography mass spectrometry-based profiling of phosphatidylcholine and phosphatidylethanolamine in the plasma and liver of acetaminophen-induced liver injured mice

et al. 2017

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BackgroundAcetaminophen (APAP) overdose is one of the most common causes of acute liver failure in many countries. The aim of the study was to describe the profiling of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in the plasma and liver of Acetaminophen -induced liver injured mice.MethodsA time course study was carried out using C57BL/6 mice after intraperitoneal administration of 300 mg/kg Acetaminophen 1 h, 3 h, 6 h, 12 h and 24 h. A high-throughput liquid chromatography mass spectrometry (LC-MS) lipidomic method was utilized to detect phosphatidylcholine and phosphatidylethanolamine species in the plasma and liver. The expressions of phosphatidylcholine and phosphatidylethanolamine metabolism related genes in liver were detected by quantitative Reverse transcription polymerase chain reaction (qRT-PCR) and Western-blot.ResultsFollowing Acetaminophen treatment, the content of many PC and PE species in plasma increased from 1 h time point, peaked at 3 h or 6 h, and tended to return to baseline at 24 h time point. The relative contents of almost all PC species in liver decreased from 1 h, appeared to be lowest at 6 h, and then return to normality at 24 h, which might be partly explained by the suppression of phospholipases mRNA expressions and the induction of choline kinase (Chka) expression. Inconsistent with PC profile, the relative contents of many PE species in liver increased upon Acetaminophen treatment, which might be caused by the down-regulation of phosphatidylethanolamine N-methyltransferase (Pemt).ConclusionsAcetaminophen overdose induced dramatic change of many PC and PE species in plasma and liver, which might be caused by damaging hepatocytes and interfering the phospholipid metabolism in Acetaminophen -injured liver.Electronic supplementary materialThe online version of this article (doi:10.1186/s12944-017-0540-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Liquid chromatography mass spectrometry-based profiling of phosphatidylcholine and phosphatidylethanolamine in the plasma and liver of acetaminophen-induced liver injured mice

et al. 2017

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“…Potential biomarkers for human liver cirrhosis and hepatocellular carcinoma include the LPCs C18:0, C18:2, and C18:3 (Griffin and Segall, 1987). Hepatotoxins, such as acetaminophen and gemfibrozil, modulate serum LPC levels (Huo et al, 2014;Liu et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Increased serum bile acid concentration following low-dose chronic administration of thioacetamide in rats, as evidenced by metabolomic analysis

Jeong

Kim

Shin

et al. 2015

Toxicology and Applied Pharmacology

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“…Toxicity Sodium valproate N-acetyl moieties of glycoprotein significantly increased (p < 0.01) in valproate sodium induced hepatotoxicity [143] Oncologic toxicity…”

Section: Clinical Study Topic Main Findings Referencesmentioning

confidence: 99%

“…Drug-induced hepatotoxicity is an important healthcare issue in the sense that side effects in patients can be serious. Currently, toxicity is only detected when the tissue has already been badly damaged [143]. For this reason, new markers are required to warn of the toxicity of drugs and to better monitor the treatment.…”

Section: Toxicitymentioning

confidence: 99%

“…For this reason, new markers are required to warn of the toxicity of drugs and to better monitor the treatment. Huo et al used 1 H-NMR to find that the N-acetyl moieties of glycoprotein were significantly increased (p < 0.01) in sodium valproate-induced hepatotoxicity in epileptic patients, among other metabolites such as glucose, lactate, acetoacetate, VLDLc/LDLc, lysophosphatidylcholines, phosphatidylcholines, choline, creatine, amino acids, pyruvate, and uric acid [143].…”

Section: Toxicitymentioning

confidence: 99%

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Title: Human Serum/Plasma Glycoprotein Analysis by 1H-NMR, an Emerging Method of Inflammatory Assessment

Fuertes-Martín

Correig

Vallvé

et al. 2020

JCM

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Several studies suggest that variations in the concentration of plasma glycoproteins can influence cellular changes in a large number of diseases. In recent years, proton nuclear magnetic resonance (1H-NMR) has played a major role as an analytical tool for serum and plasma samples. In recent years, there is an increasing interest in the characterization of glycoproteins through 1H-NMR in order to search for reliable and robust biomarkers of disease. The objective of this review was to examine the existing studies in the literature related to the study of glycoproteins from an analytical and clinical point of view. There are currently several techniques to characterize circulating glycoproteins in serum or plasma, but in this review, we focus on 1H-NMR due to its great robustness and recent interest in its translation to the clinical setting. In fact, there is already a marker in H-NMR representing the acetyl groups of the glycoproteins, GlycA, which has been increasingly studied in clinical studies. A broad search of the literature was performed showing a general consensus that GlycA is a robust marker of systemic inflammation. The results also suggested that GlycA better captures systemic inflammation even more than C-reactive protein (CRP), a widely used classical inflammatory marker. The applications reviewed here demonstrated that GlycA was potentially a key biomarker in a wide range of diseases such as cancer, metabolic diseases, cardiovascular risk, and chronic inflammatory diseases among others. The profiling of glycoproteins through 1H-NMR launches an encouraging new paradigm for its future incorporation in clinical diagnosis.

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An effective assessment of valproate sodium-induced hepatotoxicity with UPLC–MS and 1HNMR-based metabonomics approach

Cited by 29 publications

References 30 publications

Liquid chromatography mass spectrometry-based profiling of phosphatidylcholine and phosphatidylethanolamine in the plasma and liver of acetaminophen-induced liver injured mice

Liquid chromatography mass spectrometry-based profiling of phosphatidylcholine and phosphatidylethanolamine in the plasma and liver of acetaminophen-induced liver injured mice

Increased serum bile acid concentration following low-dose chronic administration of thioacetamide in rats, as evidenced by metabolomic analysis

Title: Human Serum/Plasma Glycoprotein Analysis by 1H-NMR, an Emerging Method of Inflammatory Assessment

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