An Eco-Friendly and Sustainable Process for Enzymatic Hydrolysis of Penicillin G in Cloud Point System

Abstract

Enzymatic hydrolysis of penicillin G by immobilized penicillin acylase in a nonionic surfactant mediated cloud point system was presented. The effect of the operation parameters on equilibrium pH of this enzymatic hydrolysis process without pH control was examined. A relatively high equilibrium pH in cloud point system without pH control can be obtained. The feasibility of recycling utilization of the nonionic surfactant, a novel green solvent, was also investigated experimentally. Enzymatic hydrolysis of penicillin G in a discrete semi-batch mode, which simulates a semi-continuous process, envisages a completely eco-friendly, sustainable and efficient process for production of 6-aminopenicillanic acid.

Clouding behaviour in surfactant systems

“…Wang et al [277] have recently utilized the cloud point system of nonionic surfactant Tergitol TMN-3 as a novel reaction medium for the hydrolysis of penicillin G (Pen G) by immobilizing penicillin acylase without pH control. The enzymatic hydrolysis of penicillin G in a discrete semi-batch mode and repeated utilization of surfactant, envisages the process as a completely eco-friendly, sustainable and efficient for production of 6-aminopenicillanic acid (APA).…”

Penicillin acylase immobilization depending on macromolecular crowding and catalysis in aqueous–organic medium

“…However, the enzyme activity of commercial immobilized PA has been reported to decrease by 80% when the pH drops from 8 to 3 in butyl acetatewater bi-phase system [7]. Moreover, although cloud point extraction partially eliminated the inhibition of the biocatalyst by PAA and shifts the reaction equilibrium towards further hydrolysis, the catalytic activity of PA preparation and the 6-APA product concentration were much lower than in the conventional process (control pH 8.0) [9,15,16].…”

Continuous Countercurrent Extractive Biocatalysis in Aqueous Surfactant Two‐Phase Systems

“…However, the suitability of aqueous surfactant two‐phase systems for extractive biocatalysis was demonstrated by Wang et al , in batch mode. To the best of our knowledge, no continuous process using the concept of in situ product removal in micellar systems is known yet.…”