An easy-to-use liquid chromatography assay for the analysis of lamotrigine in rat plasma and brain samples using microextraction by packed sorbent: Application to a pharmacokinetic study

Ventura, Sandra; Rodrigues, Márcio; Pousinho, Sarah; Falcão, Amı́lcar; Alves, Gilberto

doi:10.1016/j.jchromb.2016.09.032

Cited by 15 publications

(4 citation statements)

References 35 publications

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“…Finally, the dispersive SPE based on Dt-MMIPs or acetonitrile protein precipitation coupled to HPLC-UV was further compared by analyzing 6 real rat sera after oral administration of CBZ or LTG at a single dosage of 10 mg kg −1 . Referring to the C max of CBZ or LTG in the references, the sampling times were all set at 2 h. 27,28 As depicted in Table S6,† no obvious difference between two different sample treatment methods could be observed, however, the concentration of CBZ or LTG in rat serum treated by dispersive SPE based on Dt-MMIPs was higher than that of acetonitrile protein precipitation and more prone to the values of the references. 27,28…”

Section: Resultsmentioning

confidence: 99%

“…Referring to the C max of CBZ or LTG in the references, the sampling times were all set at 2 h. 27,28 As depicted in Table S6,† no obvious difference between two different sample treatment methods could be observed, however, the concentration of CBZ or LTG in rat serum treated by dispersive SPE based on Dt-MMIPs was higher than that of acetonitrile protein precipitation and more prone to the values of the references. 27,28…”

Section: Resultsmentioning

confidence: 99%

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Development of magnetic molecularly imprinted polymers with double templates for the rapid and selective determination of carbamazepine and lamotrigine in serum

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Development of magnetic molecularly imprinted polymers with double templates for the rapid and selective determination of carbamazepine and lamotrigine in serum

et al. 2022

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“…Regarding stationary phase selection, a reversed-phase LiChroCART R Purospher Star column (C 18 , 55 mm × 4 mm; 3 μm particle size) protected by a LiChroCART R Purospher Star pre-column (C 18 , 4 mm × 4 mm; 5 μm) was used. This selection was based not only on adequate intrinsic characteristics that are consistent with literature stationary phase properties [42], but also based on the vast application and suitable results obtained within our research group using this column type [43][44][45][46]. Regarding the mobile phase composition, ultra-pure water was first used as aqueous phase; however, due to its near neutral pH and bearing in mind that pK a values of PER and STP are 3.24 (weak base with one protonatable group) [40] and 14.2 (base with two protonatable groups) [47], respectively (Figure 1), the obtained peaks showed poor resolution and long retention times even when using different percentages of organic solvent.…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

Salting‐out assisted liquid–liquid extraction method optimized by design of experiments for the simultaneous high‐performance liquid chromatography analysis of perampanel and stiripentol in mouse matrices

Meirinho

Campos

Rodrigues

et al. 2020

J of Separation Science

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We report a high-performance liquid chromatography method development able to simultaneously determine perampanel and stiripentol, two third-generation antiepileptics whose therapeutic spectrum can potentially be extended, in several mouse matrices. A salting-out assisted liquid-liquid extraction optimized by a design of experiments approach was adopted for sample preparations. Isopropanol and magnesium sulfate were the extraction solvent and salting-out agent, respectively. Both drugs and internal standard (terbinafine) were separated using a LiChroCART R Purospher Star column (C 18 , 55 × 4 mm; 3 μm) isocratically pumped with mobile phase [1% triethylamine in water (pH 2.5) and acetonitrile (53:47, v/v)] at 1 mL/min. Stiripentol and terbinafine were detected by fluorescence at 254/372 nm and perampanel at 275/430 nm. Good linearity was demonstrated for perampanel at 1-500 ng/mL range in brain, 2-2000 ng/mL in liver and 1-2000 ng/mL in plasma and kidney (r 2 ≥ 0.9922), and for stiripentol between 10 and 2000 ng/mL in brain and 10 and 20 000 ng/mL in the remaining matrices (r 2 ≥ 0.9917). Precision (CV ≤ 15%) and accuracy (bias ±15%) were also verified, with obtained recovery values consistent with those predicted by the experimental design. This method was applied in preliminary pharmacokinetic studies to quantify perampanel or stiripentol after oral administration to mice, showing to be a promising bioanalytical tool to support future nonclinical in vivo pharmacokinetic studies.

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“…Chromatographic methods of analysis are widely used to determine lamotrigine in biological fluids. Methods of analysis of high-performance liquid chromatography (HPLC) (in plasma [2,3], in serum [4,5], in rat plasma and brain [6]), thin-layer chromatography (TLC) [7] and gas chromatography (GC) [8] for the determination of lamotrigine in biological samples are described, as well as methods of capillary electrophoresis [9], HPLC and GC [10], 1 H-NMR determination [11], TLC and planar chromatography in pharmaceutical forms. Information is provided on some spectrophotometric techniques for the determination of lamotrigine in the UV-(in pure form and in dosage forms [12], a first-order derivative UV method [13], spectrophotometry in the UV region after procedure of extraction [14]) and visible regions of the spectrum (using sulfophthalein dyes [15,16] or 4-(dimethylamino)benzaldehyde in acid medium [17] to give colouring products).…”

Section: Introductionmentioning

confidence: 99%

New, simple and express determination of lamotrigine in tablets by using diazole red 2J

Miedviedieva

Vasyuk

Korzhova

et al. 2022

SR: PS

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Epilepsy is considered one of the most common chronic neurological diseases among humans. Lamotrigine is an effective new-generation anticonvulsant, which is widely introduced to the pharmaceutical market by various drug manufacturers. That is why the urgent aim for pharmaceutical analysis is developing of high precision, valid, accessible and quantitative methods for lamotrigine in pharmaceuticals. The aim of the work is to study the optimal conditions for the reaction between lamotrigine and diazole red 2J, to define the coefficients of stoichiometric relationships in the “pharmaceutical substance – reagent” system and to develop a valid, sensitive and easy-to-follow technique for the quantitative determination of lamotrigine in pharmaceutical forms. Material and methods. As reagent and solvent, diazole red 2J of AR grade and acetone of AR grade were used. Analytical equipment: Specord 200 spectrophotometer, ABT-120-5DM electronic scales, Elmasonic E 60H ultrasonic bath and measuring glassware of A class. Results. A new spectrophotometric method for the quantitative determination of lamotrigine in four pharmaceutical formulations based on interaction with diazole red 2J in acetone medium has been developed. The proposed method is valid according to such validation characteristics as linearity, precision, intra-laboratory precision, accuracy, application range and robustness. The subordination to Behr's law is in the range of concentrations of 2.20–3.36 mg/100 ml. The LOD and LOQ values based on the values of the calibration line were 0.00450 % and 0.0138 % respectively. It was found that the studied coloured solutions are stable for at least 60 min and fluctuations in the amount of added red diazole 2J solution within ±10 % do not significantly affect the value of optical density. The coefficients of stoichiometric ratios between the components of the “lamotrigine – diazole red 2J” reaction mixture were defined by three methods and are 1:1. The predicted complete uncertainty of the results of analysis for quantitative determination of lamotrigine in the pharmaceutical form (2.2 %) does not exceed the maximum allowable uncertainty for the technique (3.2 %) and meets the SPhU requirements. Conclusions. According to the experimental data, the technique can be correctly reproduced and it is suitable for using in laboratories of the State Inspection for Quality Control of Medicines and QCD of the chemicopharmaceutical enterprises

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An easy-to-use liquid chromatography assay for the analysis of lamotrigine in rat plasma and brain samples using microextraction by packed sorbent: Application to a pharmacokinetic study

Cited by 15 publications

References 35 publications

Development of magnetic molecularly imprinted polymers with double templates for the rapid and selective determination of carbamazepine and lamotrigine in serum

Development of magnetic molecularly imprinted polymers with double templates for the rapid and selective determination of carbamazepine and lamotrigine in serum

Salting‐out assisted liquid–liquid extraction method optimized by design of experiments for the simultaneous high‐performance liquid chromatography analysis of perampanel and stiripentol in mouse matrices

New, simple and express determination of lamotrigine in tablets by using diazole red 2J

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