An autoradiographic study of the mouse olfactory epithelium: Evidence for long‐lived receptors

Hinds, James W.; Hinds, Patricia L.; McNelly, Nancy A.

doi:10.1002/ar.1092100213

Cited by 255 publications

(182 citation statements)

References 20 publications

(24 reference statements)

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“…Studies of neurogenesis and induced neuronal regeneration in adult animals indicate that the neuronal stem cell resides in the basal compartment of the OE (Camara and Harding, 1984;Gordon et al, 1995;Graziadei, 1973;Hinds et al, 1984;Mackay-Sim and Kittel, 1991;Moulton and Fig. 1.…”

Section: Progenitor Cells Of the Olfactory Receptor Neuron Lineage Nementioning

confidence: 99%

Progenitor cells of the olfactory receptor neuron lineage

Calof

Bonnin

Crocker

et al. 2002

Microscopy Res & Technique

130

114

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The olfactory epithelium of the mouse has many properties that make it an ideal system for studying the molecular regulation of neurogenesis. We have used a combination of in vitro and in vivo approaches to identify three distinct stages of neuronal progenitors in the olfactory receptor neuron lineage. The neuronal stem cell, which is ultimately responsible for continual neuron renewal in this system, gives rise to a transit amplifying progenitor identified by its expression of a transcription factor, MASH1. The MASH1-expressing progenitor gives rise to a second transit amplifying progenitor, the Immediate Neuronal Precursor, which is distinct from the stem cell and MASH1-expressing progenitor, and gives rise quantitatively to olfactory receptor neurons. Regulation of progenitor cell proliferation and differentiation occurs at each of these three cell stages, and growth factors of the fibroblast growth factor (FGF) and bone morphogenetic protein (BMP) families appear to play particularly important roles in these processes. Analyses of the actions of FGFs and BMPs reveal that negative signaling plays at least as important a role as positive signaling in the regulation of olfactory neurogenesis.

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Section: Progenitor Cells Of the Olfactory Receptor Neuron Lineage Nementioning

confidence: 99%

Progenitor cells of the olfactory receptor neuron lineage

Calof

Bonnin

Crocker

et al. 2002

Microscopy Res & Technique

130

114

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“…Another study has implicated the Bcl-2 protein in neuronal turnover under basal conditions (Hayward and Morgan, 1995). Mature olfactory receptor neurons normally live at least 90 d, but when mice are reared in a laminar flow hood to prevent rhinitis, olfactory receptor neurons can survive as long as 12 months (Hinds et al, 1984). Therefore, the turnover of olfactory receptor neurons may be regulated by environmental factors.…”

Section: Receptor Turnover In Other Sensory Systemsmentioning

confidence: 99%

Inhibition of Caspases Prevents Ototoxic and Ongoing Hair Cell Death

2002

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Sensory hair cells die after acoustic trauma or ototoxic insults, but the signal transduction pathways that mediate hair cell death are not known. Here we identify several important signaling events that regulate the death of vestibular hair cells. Chick utricles were cultured in media supplemented with the ototoxic antibiotic neomycin and selected pharmacological agents that influence signaling molecules in cell death pathways. Hair cells that were treated with neomycin exhibited classically defined apoptotic morphologies such as condensed nuclei and fragmented DNA. Inhibition of protein synthesis (via treatment with cycloheximide) increased hair cell survival after treatment with neomycin, suggesting that hair cell death requires de novo protein synthesis. Finally, the inhibition of caspases promoted hair cell survival after neomycin treatment.Sensory hair cells in avian vestibular organs also undergo continual cell death and replacement throughout mature life. It is unclear whether the loss of hair cells stimulates the proliferation of supporting cells or whether the production of new cells triggers the death of hair cells. We examined the effects of caspase inhibition on spontaneous hair cell death in the chick utricle. Caspase inhibitors reduced the amount of ongoing hair cell death and ongoing supporting cell proliferation in a dosedependent manner. In isolated sensory epithelia, however, caspase inhibitors did not affect supporting cell proliferation directly. Our data indicate that ongoing hair cell death stimulates supporting cell proliferation in the mature utricle.

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“…In other words, for this assumption to hold true, cells of the basal compartment must be evenly (i.e., asynchronously) distributed throughout the cell cycle. In the context of the 2 h r duration of the experiment, this assumption is reasonable even though the proliferation rate of cells of the olfactory basal compartment displays some degree of circadian variation in mice (Hinds et al, 1984). Indeed, we timed the in- jection of the first analogue 1 hr in advance of the reported peak of proliferation and the injection of the second analogue 1 hr following the peak.…”

Section: Sequential Injections Of Idu and Brdu For S Phase Determinationmentioning

confidence: 99%

“…Moreover, following injury to the olfactory system, neurogenesis in the olfactory epithelium is greatly enhanced, allowing for a rapid reconstitution of the damaged epithelium (Nagahara, 1940;Graziadei, 1973;Graziadei and DeHan, 1973;Monti Graziadei and Graziadei, 1979;Costanzo and Graziadei, 1983;Schwartz Levey et al, 1991;Schwob et al, 1995). Several lines of evidence indicate that the mitotically active cells of the basal compartment are responsible for the generation of new neurons in the olfactory epithelium Hinds et al, 1984;Mackay-Sim and Kittel, 1991;Caggiano et al, 1994). Thus, the study of cells of the basal compartment under normal circumstances and following injury to the olfactory system offers the opportunity to investigate the regulation of neurogenesis.…”

Section: Introductionmentioning

confidence: 99%

Cell cycle of globose basal cells in rat olfactory epithelium

Huard

Schwob

1995

Developmental Dynamics

112

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The olfactory epithelium of adult mammals has the unique property of generating olfactory sensory neurons throughout life. Cells of the basal compartment, which include horizontal and globose basal cells, are responsible for the ongoing process of neurogenesis in this system. We report here that the globose basal cells in olfactory epithelium of rats, as in mice, are the predominant type of proliferating cell, and account for 97.6% of the actively dividing cells in the basal compartment of the normal epithelium. Globose basal cells have not been fully characterized in terms of their proliferative properties, and the dynamic aspects of neurogenesis are not well understood. As a consequence, it is uncertain whether cell kinetic properties are under any regulation that could affect the rate of neurogenesis. To address this gap in our knowledge, we have determined the duration of both the synthesis phase (S-phase) and the full cell cycle of globose basal cells in adult rats. The duration of the S-phase was found to be 9 hr in experiments utilizing sequential injections of either IdU followed by BrdU or 3H-thy followed by BrdU. The duration of the cell cycle was determined by varying the time interval between the injections of 3H-thy and BrdU and tracking the set of cells that exit S shortly after the first injection. With this paradigm, the interval required for these cells to traverse G2, M, G1, and a second S-phase, is equivalent to the duration of one mitotic cycle and equals 17 hr. These observations serve as the foundation to assess whether the cell cycle duration is subject to regulation in response to experimental injury, and whether such regulation is partly responsible for changes in the rate of neurogenesis in such settings. o 1995 Wiley-Liss, Inc.

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An autoradiographic study of the mouse olfactory epithelium: Evidence for long‐lived receptors

Cited by 255 publications

References 20 publications

Progenitor cells of the olfactory receptor neuron lineage

Progenitor cells of the olfactory receptor neuron lineage

Inhibition of Caspases Prevents Ototoxic and Ongoing Hair Cell Death

Cell cycle of globose basal cells in rat olfactory epithelium

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