An automatic sample loader for column chromatography

Lindley, H.; Cranston, Robin; Sutherland, William J.; Ritchie, Caroline

doi:10.1016/s0021-9673(01)81249-8

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1974

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“…Amino acid analyses were carried out on a Spinco 120C analyser equipped with an automatic sampleloading facility (Lindley et al, 1973).…”

Section: General Methodsmentioning

confidence: 99%

The reactivity of the disulphide bonds of wool

Lindley

Cranston

1974

Biochemical Journal

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1. Fully reduced and S-carboxymethylated wool samples were prepared in which either the readily reducible cystine bonds or those that could only be reduced with difficulty were specifically labelled with iodo[2-(14)C]acetate; these two cystine fractions correspond to the (A+B) and (C+D) cystine fractions respectively, of Middlebrook & Phillips (1942). 2. Radioactively labelled peptides were isolated from partial acid hydrolysates of these wool samples. 3. It appears that the (A+B) cystine residues probably owe their increased reactivity to being in a more polar environment. 4. The implication of these results for the problem of characterizing the disulphide bonds of wool is briefly discussed.

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“…Amino acid analyses were carried out on a Spinco 120C analyser equipped with an automatic sampleloading facility (Lindley et al, 1973).…”

Section: General Methodsmentioning

confidence: 99%