An automatic, on-line glucose analyzer for feed-back control of fed-batch growth of Escherichia coli

Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations

“…The fermentation hardware has been extensively described elsewhere (18,28). Fermentors (MF-214; New Brunswick Scientific Co., Inc., Edison, N.J.) equipped with 14-liter vessels (working volume, 10 liters) were used throughout this study.…”

Principles and Applications of Biosensors for Bioprocess Monitoring and Control

“…The total sugars were estimated from consumption of oxygen by the cells. Luli et al (1987) reported a computer-assisted on-line glucose analyzer for feedback control of E. coli cell growth in fed-batch cultures. Various other microbial biosensors based on the same principle, that is, cells coupled to an oxygen electrode, have been described for the determination of glucose, acetic acid, or alcohol in fermentation broth (Karube, 1990).…”

Control of acetic acid concentration by pH-stat continuous substrate feeding in heterotrophic culture phase of two-stage cultivation ofAlcaligenes eutrophus for production of P(3HB) from CO2, H2, and O2 under non-explosive conditions

“…In fermentation processes where cell growth and/or product formation is inhibited by high substrate concentration or by the accumulation of a byproduct, substrate is intermittently fed to the culture system in order to maintain the substrate concentration below a certain level for enhancement of biological and metabolic activity. The feeding of substrate solution is often controlled by means of DO-stat (Mori et al, 1979;Yano et al, 1978) or pH-stat methods (Suzuki et al, 1990;Yamane et al, 1996), or by monitoring the glucose concentration with an on-line glucose analyzer when glucose is used as substrate (Kim et al, 1994;Luli et al, 1987). However, in the culture system of A. eutrophus with acetic acid as carbon source, it was very difficult to control the acetate concentration in the fermenter by a substrate feeding strategy because the optimum acetate concentration for cell growth is in a very narrow range around 1.0 g и dm −3 , and a slight increase or a slight decrease in acetate concentration will seriously suppress the cell growth.…”