An automatic dehydrogenase‐based flow‐injection system: Application for the continuous determination of glucose and lactate in mammalian cell‐cultures

Becker, Thomas; Schuhmann, Wolfgang; Betken, R.; Schmidt, Hanns‐Ludwig; Leible, Michael B.; Albrecht, A.

doi:10.1002/jctb.280580212

Cited by 10 publications

References 21 publications

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Data model for the elimination of matrix effects in enzyme-based flow-injection systems

Becker¹,

Schmidt²

2000

Biotechnol. Bioeng.

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This contribution presents a new conceptional enzyme‐based flow injection analysis (FIA) system for the process and quality control of food processing and biotechnological systems. It provides the determination of different analytes in distinct process media on the base of a common experimental set‐up. In contrast to known comparable systems, analysis is performed without the commonly used sample preparation and dilution steps. Instead, the adaptation to the necessary measurement range is realized by optimization of intrinsic system parameters. The central principle of the work presented is the elimination of occurring interferences by the heterogenous matrix of the process sample. Based on a particular injection mode, the application of dehydrogenases as indicator enzymes and a specially developed data model using cognitive methods, cross sensitivities of the detector as well as disturbed reaction rates of the enzymes could be almost completely compensated. Two applications are presented, the analysis of ethanol in non‐alcoholic beer and the online determination of D‐/L‐lactate during a lactic acid fermentation, which reveal the advantage of the developed system. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 69: 377–384, 2000.

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Data model for the elimination of matrix effects in enzyme-based flow-injection systems

Becker¹,

Schmidt²

2000

Biotechnol. Bioeng.

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Improvement of substrate‐selectivity in enzyme‐linked FIA‐systems by differential measurement and ‘enzyme‐array’

Englbrecht

Schmidt

1995

J of Chemical Tech & Biotech

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Flow injection systems with immobilized enzymes for the parallel determination of chemically similar substances such as xylose/glucose and L‐leucine/L‐isoleucine/L‐valine are described. The determination of xylose and glucose was performed either by differential measurement or by an ‘enzyme‐array’. Because of the different linear ranges of the calibration curves for xylose and glucose quantification was only possible when there was an excess of xylose (xylose 0·1–2·5 mmol dm−3, glucose 0·001–0·1 mmol dm−3). The parallel quantification of the branched‐chain amino acids could be realized by an array arrangement using an FIA‐system with several immobilized enzymes of slightly different selectivity. The total amount of branched‐chain amino acids can be up to about 1·1 mmol dm−3.

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Martens

End

2007

Animal Cell Biotechnology

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An automatic dehydrogenase‐based flow‐injection system: Application for the continuous determination of glucose and lactate in mammalian cell‐cultures

Cited by 10 publications

References 21 publications

Data model for the elimination of matrix effects in enzyme-based flow-injection systems

Data model for the elimination of matrix effects in enzyme-based flow-injection systems

Improvement of substrate‐selectivity in enzyme‐linked FIA‐systems by differential measurement and ‘enzyme‐array’

Configuration of Bioreactors

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