An automated turbulent flow liquid chromatography–isotope dilution mass spectrometry (LC–IDMS) method for quantitation of serum creatinine

Harlan, Robert; Clarke, William; Bussolo, Joseph M. Di; Kozák, M.; Straseski, Joely A.; Meany, Danni L.

doi:10.1016/j.cca.2010.07.013

Cited by 52 publications

(28 citation statements)

References 6 publications

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“…The IDMS creatinine method has been described (8 ) and is traceable to serum-based NIST calibrators (Standard Reference Material 967a, levels 1 and 2).…”

Section: Creatinine Methodsmentioning

confidence: 99%

“…To confirm the performance of the core laboratory method (Roche Modular enzymatic assay), frozen aliquots of the initial patient samples were assayed using an IDMS creatinine method (8 ), and results were compared with both enzymatic (Fig. 3A) and StatSensor (Fig.…”

Section: Enzymatic Methods Confirmationmentioning

confidence: 99%

“…In the past, comparisons of novel whole-blood creatinine methods with plasma enzymatic creatinine reference methods were susceptible to systematic bias, because of the lack of creatinine standardization. To address these concerns and reduce the risk of reference assay interference, we compared POC results to an isotope-dilution mass spectrometry (IDMS) creatinine assay (8 ).…”

confidence: 99%

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Investigating Interferences of a Whole-Blood Point-of-Care Creatinine Analyzer: Comparison to Plasma Enzymatic and Definitive Creatinine Methods in an Acute-Care Setting

et al. 2011

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“…The IDMS creatinine method has been described (8 ) and is traceable to serum-based NIST calibrators (Standard Reference Material 967a, levels 1 and 2).…”

Section: Creatinine Methodsmentioning

confidence: 99%

Section: Enzymatic Methods Confirmationmentioning

confidence: 99%

See 1 more Smart Citation

Investigating Interferences of a Whole-Blood Point-of-Care Creatinine Analyzer: Comparison to Plasma Enzymatic and Definitive Creatinine Methods in an Acute-Care Setting

et al. 2011

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“…We took advantage of online sample clean-up using multidimensional chromatography that eliminates time-consuming and laborious protocol steps such as LLE and SPE, the use of toxic and volatile organic solvents such as ethyl acetate [3,19,20], and reduce the need for large amounts of consumables. Benefits of using TFC were described in a large number of varied analytical environments, drug discovery and pharmacokinetics, metabolite profiling, and clinical applications [10,21]. The combination of both a TFC-column and an analytical UHPLC column, improved the multiplexed enzymatic assay by eliminating interfering compounds and by separating enzymatic products from residual substrates.…”

Section: Implication For Early Detection Strategies For Lysosomal Stomentioning

confidence: 99%

Short-incubation mass spectrometry assay for lysosomal storage disorders in newborn and high-risk population screening

Mechtler

Metz

Müller

et al. 2012

Journal of Chromatography B

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The interest in early detection strategies for lysosomal storage disorders (LSDs) in newborns and high-risk population has increased in the last years due to the availability of novel treatment strategies coupled with the development of diagnostic techniques. We report the development of a short-incubation mass spectrometry-based protocol that allows the detection of Gaucher, Niemann-Pick A/B, Pompe, Fabry and mucopolysaccharidosis type I disease within 4h including sample preparation from dried blood spots. Optimized sample handling without the need of time-consuming offline preparations, such as liquid-liquid and solid-phase extraction, allows the simultaneous quantification of five lysosomal enzyme activities using a cassette of substrates and deuterated internal standards. Applying incubation times of 3h revealed in intra-day CV% values ranging from 4% to 11% for all five enzyme activities, respectively. In a first clinical evaluation, we tested 825 unaffected newborns and 16 patients with LSDs using a multiplexed, turbulent flow chromatography-ultra high performance liquid chromatography-tandem mass spectrometer assay. All affected patients were identified accurately and could be differentiated from non-affected newborns. In comparison to previously published two-day assays, which included an overnight incubation, this protocol enabled the detection of lysosomal enzyme activities from sample to first result within half a day.

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“…Morover, picric acid is poisonous and explosive, which may damage the health of operator. Other methods for the quantification of creatinine, including high performance liquid chromatography [4], capillary zone electrophoresis [5], high performance capillary electrophoresis [6], liquid chromatography-isotope dilution mass spectrometry [7], amperometry [8], and potentiometry [9], have been developed. Although these assays have highly selectivity, sensitivity and accuracy in the determination of creatinine, they are either time-consuming and expensive, or required sophisticated instruments.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles-based colorimetric and visual creatinine assay

Zhang

2015

Microchim Acta

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We demonstrate a selective and sensitive method for determination of creatinine using citrate-stabilized gold nanoparticles (AuNPs) as a colorimetric probe. It is based on a direct cross-linking reaction that occurs between creatinine and AuNPs that causes aggregation of AuNPs and results in a color change from wine red to blue. The absorption peak is shifted from 520 to 670 nm. Under the optimized conditions, the shift in the absorption peak is related the logarithm of the creatinine concentration in the 0.1 to 20 mM range, and the instrumental detection limit (LOD) is 80 μM. This LOD is about one order of magnitude better than that that of the Jaffé method (720 μM). The assay displays good selectivity over interfering substances including various inorganic ions, organic small compounds, proteins, and biothiols. It was successfully employed to the determination of creatinine in spiked human urine.

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An automated turbulent flow liquid chromatography–isotope dilution mass spectrometry (LC–IDMS) method for quantitation of serum creatinine

Cited by 52 publications

References 6 publications

Investigating Interferences of a Whole-Blood Point-of-Care Creatinine Analyzer: Comparison to Plasma Enzymatic and Definitive Creatinine Methods in an Acute-Care Setting

Investigating Interferences of a Whole-Blood Point-of-Care Creatinine Analyzer: Comparison to Plasma Enzymatic and Definitive Creatinine Methods in an Acute-Care Setting

Short-incubation mass spectrometry assay for lysosomal storage disorders in newborn and high-risk population screening

Gold nanoparticles-based colorimetric and visual creatinine assay

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