An automated solid phase extraction procedure for lipid biomarker purification and stable isotope analysis

Rach, Oliver; Hadeen, Xennophone; Sachse, Dirk

doi:10.1016/j.orggeochem.2020.103995

Cited by 20 publications

(16 citation statements)

References 24 publications

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“…In addition, the total lipid content of twenty-three plant samples were extracted by mean of sonication and a mixture of dichloromethane: methanol (9:1). The aliphatic fraction (including the n-alkanes) was eluted with hexane and separated by means of manual silica gel chromatography following the procedure described in Rach et al (2020). The n-alkanes were identified and quantified using Gas Chromatography flame ionization detection and mass spectrometry (GC-FID and GC-MS) following Rach et al (2020) settings.…”

Section: Organic Geochemical Analysesmentioning

confidence: 99%

“…The aliphatic fraction (including the n-alkanes) was eluted with hexane and separated by means of manual silica gel chromatography following the procedure described in Rach et al (2020). The n-alkanes were identified and quantified using Gas Chromatography flame ionization detection and mass spectrometry (GC-FID and GC-MS) following Rach et al (2020) settings. Another purification step using pre-activated Cu powder (to remove potential sulphurs) and preactivated AgNO 3 (to separate saturated and unsaturated hydrocarbons) was performed in ten samples to assess if this extra step would significantly improve the n-alkane signal for hydrogen isotope analyses.…”

Section: Organic Geochemical Analysesmentioning

confidence: 99%

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Paleohydrological dynamics in the Western Mediterranean during the last glacial cycle

García‐Alix

Camuera

Ramos‐Román

et al. 2021

Global and Planetary Change

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Section: Organic Geochemical Analysesmentioning

confidence: 99%

Section: Organic Geochemical Analysesmentioning

confidence: 99%

Paleohydrological dynamics in the Western Mediterranean during the last glacial cycle

García‐Alix

Camuera

Ramos‐Román

et al. 2021

Global and Planetary Change

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“…Total lipid extracts were recovered using an accelerated solvent extraction system (Dionex ASE) with 9:1 v/v dichloromethane:methanol. The n ‐alkane fraction was isolated using the manual solid phase extraction (Rach et al., 2020) and quantified using an Agilent gas chromatograph (GC 7890‐A) with flame ionization detection coupled to a single quadrupole mass spectrometer (MS 5975‐C). We measured n ‐alkane δ 13 C via gas chromatography‐combustion‐isotope ratio mass spectrometry (GC‐C‐IRMS; Agilent 7890N, ThermoFisher Delta V Plus).…”

Section: Study Area and Methodsmentioning

confidence: 99%

River Organic Carbon Fluxes Modulated by Hydrodynamic Sorting of Particulate Organic Matter

Repasch

Scheingross

Hovius

et al. 2022

Geophysical Research Letters

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Cycling of organic carbon (OC) between the lithosphere, biosphere, and atmosphere regulates Earth's climate. Rivers modulate this cycle by redistributing OC from vegetated uplands to sedimentary basins. If fluvially transported OC is preserved during source-to-sink transit, its long-term burial sequesters atmospheric CO 2 over geologic timescales (Berner, 1990;Burdige, 2005;France-Lanord & Derry, 1997). To estimate OC burial fluxes and assess the influence of fluvial transport on the geologic carbon cycle, we need mechanistic explanations of the complex links between the geomorphic and biogeochemical processes driving the sourcing, transport, oxidation, and burial of OC in river systems.Fluvial particulate organic carbon (POC) comprises a mixture of petrogenic POC from organic-rich bedrock (e.g., shale) and biospheric POC from plants and soils (Galy et al., 2015;Rosenheim & Galy, 2012). While petrogenic POC decomposes slowly and can be preserved and recycled through the lithosphere over million-year cycles (Galy, et al., 2008;Scheingross et al., 2019;Sparkes et al., 2020), biospheric POC is physically and chemically unstable at Earth's surface and can be oxidized to CO 2 if not rapidly buried. Biospheric POC can bind

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“…We measured compound-specific 14 C content of leaf wax n-alkanes to trace the radiocarbon signature of vascular plant-derived POC. Lipid compounds were extracted from bulk sediment using a Dionex accelerated solvent extraction system (ASE 350), and the n-alkanes were further isolated by solid-phase extraction over silica gel columns following the manual procedure of Rach et al 54 . The C 27 , C 29 , C 31 and C 33 n-alkanes were then purified from the saturated lipid fraction using preparative capillary gas chromatography following Eglinton et al 55 .…”

Section: Methodsmentioning

confidence: 99%

Fluvial organic carbon cycling regulated by sediment transit time and mineral protection

et al. 2021

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Rivers transfer terrestrial organic carbon (OC) from mountains to ocean basins, playing a key role in the global carbon cycle. During fluvial transit, OC may be oxidized and emitted to the atmosphere as CO2 or preserved and transported to downstream depositional sinks. The balance between oxidation and preservation determines the amount of particulate OC (POC) that can be buried long term, but the factors regulating this balance are poorly constrained. Here, we quantify the effects of fluvial transit on POC fluxes along an ~1,300 km lowland channel with no tributaries. We show that sediment transit time and mineral protection regulate the magnitude and rate of POC oxidation, respectively. Using a simple turnover model, we estimate that annual POC oxidation is a small percentage of the POC delivered to the river. Modelling shows that lateral erosion into POC-rich floodplains can increase POC fluxes to downstream basins, thereby offsetting POC oxidation. Consequently, rivers with high channel mobility can enhance CO2 drawdown while management practices that stabilize river channels may reduce the potential for CO2 drawdown.

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An automated solid phase extraction procedure for lipid biomarker purification and stable isotope analysis

Cited by 20 publications

References 24 publications

Paleohydrological dynamics in the Western Mediterranean during the last glacial cycle

Paleohydrological dynamics in the Western Mediterranean during the last glacial cycle

River Organic Carbon Fluxes Modulated by Hydrodynamic Sorting of Particulate Organic Matter

Fluvial organic carbon cycling regulated by sediment transit time and mineral protection

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