An automated online three-phase electro-extraction setup with machine-vision process monitoring hyphenated to LC-MS analysis

He, Yangyan; Miggiels, Paul; Drouin, Nicolas; Lindenburg, Peter W.; Wouters, Bert; Hankemeier, Thomas

doi:10.1016/j.aca.2022.340521

Cited by 3 publications

(2 citation statements)

References 52 publications

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“…Since microsamples comprise only a few hundred microliters, highly sensitive bioanalytical assays are required for analysis in addition to improved sample clean‐up, particularly for low‐abundant target analytes. In addition to the conventional sample clean‐up procedures, microsample preparation has been enhanced by the development of new techniques such as porous polymeric thin‐film extraction, 16 micro‐SPE with pipette tips and spin columns 17 and three‐phase electroextraction, which is capable of achieving simultaneous clean‐up and high enrichment of 20 µL microsamples in a short amount of time 18 . Automation of miniaturised extraction techniques and direct hyphenation to analytical instrumentation minimises sample loss and alleviates the sample‐preparation bottlenecks in volume‐limited samples 19 .…”

Section: Introductionmentioning

confidence: 99%

“…In addition to the conventional sample clean‐up procedures, microsample preparation has been enhanced by the development of new techniques such as porous polymeric thin‐film extraction, 16 micro‐SPE with pipette tips and spin columns 17 and three‐phase electroextraction, which is capable of achieving simultaneous clean‐up and high enrichment of 20 µL microsamples in a short amount of time. 18 Automation of miniaturised extraction techniques and direct hyphenation to analytical instrumentation minimises sample loss and alleviates the sample‐preparation bottlenecks in volume‐limited samples. 19 The most extensively employed quantitative analytical technique for microsample analysis is liquid chromatography (LC) separation coupled with mass spectrometry (MS) detection due to its ability to detect analytes in pg/mL concentrations.…”

Section: Introductionmentioning

confidence: 99%

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Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

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With the development of highly sensitive bioanalytical techniques, the volume of samples necessary for accurate analysis has reduced. Microsampling, the process of obtaining small amounts of blood, has thus gained popularity as it offers minimal‐invasiveness, reduced logistical costs and biohazard risks while simultaneously showing increased sample stability and a potential for the decentralization of the approach and at‐home self‐sampling. Although the benefits of microsampling have been recognised, its adoption in clinical practice has been slow. Several microsampling technologies and devices are currently available and employed in research studies for various biomedical applications. This review provides an overview of the state‐of‐the‐art in microsampling technology with a focus on the latest developments and advancements in the field of microsampling. Research published in the year 2022, including studies (i) developing strategies for the quantitation of analytes in microsamples and (ii) bridging and comparing the interchangeability between matrices and choice of technology for a given application, is reviewed to assess the advantages, challenges and limitations of the current state of microsampling. Successful implementation of microsampling in routine clinical care requires continued efforts for standardization and harmonization. Microsampling has been shown to facilitate data‐rich studies and a patient‐centric approach to healthcare and is foreseen to play a central role in the future digital revolution of healthcare through continuous monitoring to improve the quality of life.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

Self Cite

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Modern automated sample preparation for the determination of organic compounds: A review on robotic and on-flow systems

Medina

Maciel

Lanças

2023

TrAC Trends in Analytical Chemistry

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Automatization of metabolite extraction for high-throughput metabolomics: case study on transgenic isoprene-emitting birch

Bertić,

Zimmer,

Andrés-Montaner

et al. 2023

Tree Physiology

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Metabolomics studies are becoming increasingly common for understanding how plant metabolism responds to changes in environmental conditions, genetic manipulations, and treatments. Despite the recent advances in metabolomics workflow, the sample preparation process still limits the high-throughput analysis in large-scale studies. Here, we present a highly flexible robotic system that integrates liquid handling, sonication, centrifugation, solvent evaporation, and sample transfer processed in 96-well plates to automatize the metabolite extraction from leaf samples. We transferred an established manual extraction protocol performed to a robotic system, and with this, we show the optimization steps required to improve reproducibility and obtain comparable results in terms of extraction efficiency and accuracy. We then tested the robotic system to analyze the metabolomes of wild-type and four transgenic silver birch (Betula pendula) lines under unstressed conditions. Birch trees were engineered to overexpress the poplar (Populus x canescens) isoprene synthase (PcISPS) and emit various amounts of isoprene. By fitting the different isoprene emission capacities of the transgenic trees with their leaf metabolomes, we observed an isoprene-dependent upregulation of some flavonoids and other secondary metabolites as well as carbohydrates, amino acid and lipid metabolites. In contrast, the disaccharide sucrose was found to be strongly negatively correlated to isoprene emission. The presented study illustrates the power of integrating robotics to increase sample throughput, reduce human errors and labor time, and ensure a fully controlled, monitored, and standardized sample preparation procedure. Due to its modular and flexible structure, the robotic system can be easily adapted to other extraction protocols for the analysis of various tissues or plant species to achieve high-throughput metabolomics in plant research.

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An automated online three-phase electro-extraction setup with machine-vision process monitoring hyphenated to LC-MS analysis

Cited by 3 publications

References 52 publications

Blood microsampling technologies: Innovations and applications in 2022

Blood microsampling technologies: Innovations and applications in 2022

Modern automated sample preparation for the determination of organic compounds: A review on robotic and on-flow systems

Automatization of metabolite extraction for high-throughput metabolomics: case study on transgenic isoprene-emitting birch

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