An automated maze task for assessing hippocampus-sensitive memory in mice

Pioli, Elsa Y.; Gaskill, Brianna N.; Gilmour, Gary; Tricklebank, Mark D.; Dix, Sophie; Bannerman, David M.; Garner, Joseph P.

doi:10.1016/j.bbr.2013.12.009

Cited by 53 publications

(56 citation statements)

References 36 publications

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“…First, we found that spatial alternation, a putative index of mnemonic function, was impaired in Dp10Yey mice. In contrast, trial latency, which provides an independent measure of cognitive processing that includes decision-making, planning, goaldirected behavior, and attention (Bizon et al, 2012;Pioli et al, 2014), was prolonged in Dp1Tyb mice. In addition, we have shown that Dp1Tyb mice have a lower peak frequency in the theta band in the hippocampus and mPFC, an increase in PAC between theta and HG in the hippocampus, and a striking increase in theta phase coupling between the mPFC and hippocampus-each of which is independent of the observed differences in movement statistics between those animals and their WT littermates.…”

Section: Discussionmentioning

confidence: 91%

“…We hypothesized that these trisomic mice might exhibit distinct cognitive impairments, corresponding to distinct alterations in oscillatory activity patterns within the hippocampus and mPFC (Anderson et al, 2013;Ruiz-Mejias et al, 2016). Hence, we carried out simultaneous local field potential (LFP) recordings from those regions while mice performed a canonical test of spatial working memory-the spontaneous alternation task-which, importantly, can dissociate mnemonic function (i.e., alternation success; Deacon and Rawlins, 2006;Sarnyai et al, 2000;Wenk, 2001) from planning and decisionmaking processes (i.e., trial latency; Bizon et al, 2012;Pioli et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Altered Hippocampal-Prefrontal Neural Dynamics in Mouse Models of Down Syndrome

et al. 2020

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Graphical Abstract Highlights d Study of behavior and EEG in three partially trisomic mouse models of Down syndrome (DS) d These mutant mice show non-overlapping differences in spatial working memory function d Behavioral changes segregate with distinct EEG abnormalities in the hippocampus and mPFC d This links cognitive deficits to specific changes in hippocampal and mPFC circuit dynamics SUMMARYAltered neural dynamics in the medial prefrontal cortex (mPFC) and hippocampus may contribute to cognitive impairments in the complex chromosomal disorder Down syndrome (DS). Here, we demonstrate non-overlapping behavioral differences associated with distinct abnormalities in hippocampal and mPFC electrophysiology during a canonical spatial working memory task in three partially trisomic mouse models of DS (Dp1Tyb, Dp10Yey, and Dp17Yey) that together cover all regions of homology with human chromosome 21 (Hsa21). Dp1Tyb mice show slower decision-making (unrelated to the gene dose of DYRK1A, which has been implicated in DS cognitive dysfunction) and altered theta dynamics (reduced frequency, increased hippocampal-mPFC coherence, and increased modulation of hippocampal high gamma); Dp10Yey mice show impaired alternation performance and reduced theta modulation of hippocampal low gamma; and Dp17Yey mice are not significantly different from the wild type. These results link specific hippocampal and mPFC circuit dysfunctions to cognitive deficits in DS models and, importantly, map them to discrete regions of Hsa21.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Altered Hippocampal-Prefrontal Neural Dynamics in Mouse Models of Down Syndrome

et al. 2020

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“…This result is consistent with the time spent in the target quadrant of the MWM, supporting the long term memory enhancing potential of the extract. To confirm the potential of V. subterranea aqueous extract on both scopolamine-induced retention and spatial short-term memory deficits, the T-maze test, well know sensitive tool for the detection of hippocampal dependent dysfunction was used [55]. Treatment with the aqueous extract could increase the time spent in the preferred arm associated with a decrease in the time spent in the discriminated arm and number of initial arm return.…”

Section: Effect Of V Subterranea On Hippocampi Cell Architecturementioning

confidence: 99%

Cholinergic Enhancing and Antioxidant Effect of Vigna subterranea (L.) Verdc. (Fabaceae) Landrace Aqueous Extract on Scopolamine-Induced Amnesia in Male Swiss Mice

Abaïssou¹,

Foyet²,

Wado³

et al. 2020

Rev.Chim.

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The present study was conducted to investigate protective effects of the aqueous extract of V. subterranea seeds landrace on amnesia induced by scopolamine in mice. V. subterranea aqueous extract (100, 200 and 400 mg/kg BW) was administered by gavage for nine consecutive days and memory impairment was induced by repeated intraperitoneal injection of scopolamine (1.5 mg/kg). The Y-maze (YM), Morris water maze (MWM), novel object recognition paradigm (NOR) and the T maze (TM) were used to assess learning, memory and retention. Superoxide dismutase (SOD), Catalase (CAT), Malondialdehyde (MDA) levels and Acetylcholine esterase activity was also evaluated in the mice hippocampi homogenates. V. subterranea aqueous extract (400 mg/kg) significantly increased the percentage of spontaneous alternation in the YM task and decreased escape latency in the MWM. Moreover, this dose brought about a significantly improvement in the time spent in the preferred TM arm and discrimination index in the NOR tasks despite repeated scopolamine injection. Additionally, low acetylcholine esterase levels, reduced lipid peroxidation (malondialdehyde) but increased antioxidant enzymes (catalase and superoxide dismutase) activity was observed in hippocampi homogenate of mice pre-treated with the extract. A protective action against hippocampal cell damage was also evident. This finding suggests that the aqueous extract of V. subterranea seed landrace may improve learning and memory.

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“…This requires a system where a task can be trained both with and without barriers such as automated doors. However, most automated mazes employ doors to enforce trial structure, for example to restrain the animal in the start-box before the initiation of a trial, to ensure it does not visit multiple reward areas after an incorrect behavioral response, and to direct the animal back to the start-box [26,14]. We show here that it is possible to train rats to both conform to the trial structure and perform the desired task through incremental training phases without barriers, thereby facilitating experiments where the use of barriers may influence the neural processes to be studied, yet automation of the trial structure is desired.…”

Section: Discussionmentioning

confidence: 99%

“…These unwanted effects can be largely resolved through the use of automated training systems such as B.F. Skinner's operant conditioning chamber [10]. These systems have evolved for various applications, and can include touch screens [11], acoustically transparent chambers [12] and high throughput systems implemented in the home cage [13,14]. The various custom made and commercially available automated training systems available are mostly implemented in Skinner boxes however.…”

Section: Introductionmentioning

confidence: 99%

An incremental training method with automated, extendible T-maze for training spatial behavioral tasks in rodents

Holleman

Maka

Schroeder

et al. 2019

Preprint

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We present a training procedure and a T-maze equipped with sensors and automated feeders for training spatial behavioral tasks in rodents. The maze can be transformed from an enclosed box to a maze of variable dimensions. The modularity of the protocol and setup makes it highly flexible and suitable for training a wide variety of spatial tasks, and facilitates incremental training stages of increasing maze size for more efficient learning. The apparatus, in its software and hardware, is able to adapt to animal performance, adjusting task challenges and difficulty. Two different methods of automatic behavioral scoring are evaluated against manual methods. Sensors embedded in the maze provide information regarding the order of reward locations visited and the time between the activation of the cue via the nose-poke and the activation of the reward location sensors. The distributions of these reaction times differ between correct and incorrect trials, providing an index of behavior and motivation. The automated maze system allows the trainer to operate and monitor the task away from the experimental set-up, minimizing human interference and improving the reproducibility of the experiment. We show that our method succeeds in training a binary forced-choice task in rats.The learning curve in figure 2A was acquired using manual scoring to mark a trial as correct or incorrect.

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An automated maze task for assessing hippocampus-sensitive memory in mice

Cited by 53 publications

References 36 publications

Altered Hippocampal-Prefrontal Neural Dynamics in Mouse Models of Down Syndrome

Altered Hippocampal-Prefrontal Neural Dynamics in Mouse Models of Down Syndrome

Cholinergic Enhancing and Antioxidant Effect of Vigna subterranea (L.) Verdc. (Fabaceae) Landrace Aqueous Extract on Scopolamine-Induced Amnesia in Male Swiss Mice

An incremental training method with automated, extendible T-maze for training spatial behavioral tasks in rodents

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