An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction

Tong, Rui; Zhang, Lijuan; Hu, Chuandeng; Chen, Xuee; Song, Qi; Lou, Kai; Tang, Xin; Chen, Yongsheng; Gong, Xiuqing; Gao, Yibo; Wen, Weijia

doi:10.3390/mi10030204

Cited by 13 publications

(14 citation statements)

References 26 publications

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“…All the detections were completed in about 1 hour. The microfluidic PCR detection system established in this study could meet the requirements for rapid detection of samples at the customs port [36,37]. In the case of the infectious diseases related to swine diseases, application of the microfluidic PCR technology would effectively help the inspection to be carried out on-site.…”

Section: Discussionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Zhou

Jiang²,

et al. 2020

Preprint

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Background：At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process takes a rather long time, has the risks of degradation of biological samples [32] and generation of pathogenic microorganisms [33], and does not meet the rapid on-site detection demand [34]. Therefore, development of a technology for rapid and high- throughput detection of pathogenic microorganisms at the customs port is of great significance. This study was to develop a microfluidic chip to be applied to rapid high-throughput swine disease detection with higher accuracy and lower risk of spreading pathogenic microorganisms during transportation.Results: PCR technology has the advantages of high accurate and sensitivity in disease detection, clinical testing and food quarantine, so it plays an important role in customs inspection. However, the traditional PCR detection instrument has a large size, is time-consuming and has strict requirements on the experimental environment, which greatly limit its application in on-site testing. In this paper, the clinical samples of four swine diseases were detected by a portable and rapid microfluidic PCR system, which could achieve a on- site real-time quantitative PCR detection. Eight clinical samples were detected together on the microfluidic chip in the system, and the detection results were obtained in about an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results show that the high sensitivity and specificity of the microfluidic PCR detection system in disease detection will play an important role in customs inspection and quarantine during customs clearance.Conclusion: The microfluidic PCR detection system established in this study could meet the requirements for rapid detection of samples at the customs portThe new method can avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reducing the inspection cycle, and would enable parallel inspections on one chip which greatly raising the efficiency of inspection.

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Section: Discussionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Zhou

Jiang²,

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Therefore, this chip is helpful for the further development of a multiple optical channel instrument for high-throughput multiplex PCR detection. In the future, a more highthroughput chip that could realize a more than 16 samples testing on a single chip will be developed, which could accommodate more samples for simultaneous detection, along with higher throughput, greater efficiency and accuracy.The further study also needs to develop an automated sample preparation system for nucleic acid extraction and purification, which will decrease the on-the-spot detection duration [39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Jiang¹,

Jiang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background： At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process had the risks of degradation of biological samples and generation of pathogenic microorganisms and did not meet the rapid on-site detection demand because it took a rather long time. Therefore, it is urgently needed to develop a rapid and high-throughput detection assay of pathogenic microorganisms at the customs port. The aim of this study was to develop a microfluidic chip to rapidly detect swine pathogenic microorganisms with high-throughput and higher accuracy. Moreover, this chip will decrease the risk of spreading infection during transportation. Results: A series of experiments were performed to establish a microfluidic chip. The resulting data showed that the positive nucleic acid of four swine viruses were detected by using a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Furthermore, the detection results of eight clinical samples were obtained within an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results showed that the high sensitivity and specificity of this chip system in disease detection played an important role in customs inspection and quarantine during customs clearance. Conclusion: The microfluidic PCR detection system established in this study could meet the requirement for rapid detection of samples at the customs port. This chip could avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reduce the inspection cycle. Moreover, it would enable parallel inspections on one chip, which greatly raised the efficiency of inspection.

show abstract

“…Therefore, this chip is helpful for the further development of a multiple optical channel instrument for high-throughput multiplex PCR detection. In the future, a more high-throughput chip that could realize a more than 16 samples testing on a single chip will be developed, which could accommodate more samples for simultaneous detection, along with higher throughput, greater e ciency and accuracy.The further study also needs to develop an automated sample preparation system for nucleic acid extraction and puri cation, which will decrease the on-thespot detection duration [39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Jiang¹,

Jiang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background： At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process had the risks of degradation of biological samples and generation of pathogenic microorganisms and did not meet the rapid on-site detection demand because it took a rather long time. Therefore, it is urgently needed to develop a rapid and high-throughput detection assay of pathogenic microorganisms at the customs port. The aim of this study was to develop a microfluidic chip to rapidly detect swine pathogenic microorganisms with high-throughput and higher accuracy. Moreover, this chip will decrease the risk of spreading infection during transportation.Results: A series of experiments were performed to establish a microfluidic chip. The resulting data showed that the positive nucleic acid of four swine viruses were detected by using a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Furthermore, the detection results of eight clinical samples were obtained within an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results showed that the high sensitivity and specificity of this chip system in disease detection played an important role in customs inspection and quarantine during customs clearance.Conclusion: The microfluidic PCR detection system established in this study could meet the requirement for rapid detection of samples at the customs port. This chip could avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reduce the inspection cycle. Moreover, it would enable parallel inspections on one chip, which greatly raised the efficiency of inspection.

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An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction

Cited by 13 publications

References 26 publications

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

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