An autologous epidermal equivalent tissue‐engineered from follicular outer root sheath keratinocytes is as effective as split‐thickness skin autograft in recalcitrant vascular leg ulcers

Tausche, Anne‐Kathrin; Skaria, Mouna; Böhlen, L.; Liebold, K.; Hafner, Jürg; Friedlein, Helmut; Meurer, Michael; Goedkoop, René; Wollina, Uwe; Salomon, Denis; Hunziker, Thomas

doi:10.1046/j.1524-475x.2003.11403.x

Cited by 119 publications

(66 citation statements)

References 12 publications

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“…Contact with neighboring cells in the niche can be simulated with coculture techniques. Proliferation-enhancing feeder layers for human cells include human BMSCs for early progenitor cells from umbilical cord blood, 65 growth-arrested human dermal fibroblasts for hair follicle stem cells, 66 and the murine stromal cell line AFT024 for multi-potent hematopoietic progenitors. 67 Neighboring cells can also direct differentiation; coculture with heat-shocked small airway epithelial cells causes human BMSCs to differentiate into airway epithelial-like cells.…”

Section: Cultivation Strategiesmentioning

confidence: 99%

Review:Ex VivoEngineering of Living Tissues with Adult Stem Cells

et al. 2006

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Adult stem cells have the potential to revolutionize regenerative medicine with their unique abilities to self-renew and differentiate into various phenotypes. This review examines progress and challenges in ex vivo tissue engineering with adult stem cells. These rare cells are harvested from a variety of tissues, including bone marrow, adipose, skeletal muscle, and placenta, and differentiate into cells of their own lineage and in some cases atypical lineages. Insight into the stem cell niche leads to the identification of matrix components, soluble factors, and physiological conditions that enhance the ex vivo amplification and differentiation of stem cells. Scaffolds composed of metals, naturally occurring materials, and synthetic polymers organize stem cells into complex spatial groupings that mimic native tissue. Cell signals from covalently bound ligands and slowly released regulatory factors in scaffolds direct stem cell fate. Future advances in stem cell biology and scaffold design will ultimately improve the efficacy of tissue substitutes as implants, in research, and as extracorporeal devices.

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Section: Cultivation Strategiesmentioning

confidence: 99%

Review:Ex VivoEngineering of Living Tissues with Adult Stem Cells

et al. 2006

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“…Hair follicle stem cells (HFSCs) residing in the bulge contribute to hair follicle regeneration and wound repair [19] and can differentiate to epidermis, sebaceous glands, and eight different types of hair follicle epithelial cells [20][21][22][23]. The epithelialmesenchymal interactions with the underlying dermal papilla play a pivotal role in embryonic hair genesis [24], the regulation of the postnatal hair follicle cyclical activity, and the repair of wounded skin [25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Dermal Papilla Cells Improve the Wound Healing Process and Generate Hair Bud-Like Structures in Grafted Skin Substitutes Using Hair Follicle Stem Cells

Leirós

Kusinsky

Drago³

et al. 2014

Stem Cells Translational Medicine

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Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries and might contribute to the understanding of skin regeneration. The use of dermal papilla cells (DPCs) as a dermal component in a permanent composite skin with human hair follicle stem cells (HFSCs) was evaluated by studying the tissue-engineered skin architecture, stem cell persistence, hair regeneration, and graft-take in nude mice. A porcine acellular dermal matrix was seeded with HFSCs alone and with HFSCs plus human DPCs or dermal fibroblasts (DFs). In vitro, the presence of DPCs induced a more regular and multilayered stratified epidermis with more basal p63-positive cells and invaginations. The DPC-containing constructs more accurately mimicked the skin architecture by properly stratifying the differentiating HFSCs and developing a well-ordered epithelia that contributed to more closely recapitulate an artificial human skin. This acellular dermal matrix previously repopulated in vitro with HFSCs and DFs or DPCs as the dermal component was grafted in nude mice. The presence of DPCs in the composite substitute not only favored early neovascularization, good assimilation and remodeling after grafting but also contributed to the neovascular network maturation, which might reduce the inflammation process, resulting in a better healing process, with less scarring and wound contraction. Interestingly, only DPC-containing constructs showed embryonic hair bud-like structures with cells of human origin, presence of precursor epithelial cells, and expression of a hair differentiation marker. Although preliminary, these findings have demonstrated the importance of the presence of DPCs for proper skin repair.

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“…65 Interestingly, with the exception of BM-MSCs delivered in fibrin, none of the most recent trials has tested the combination of stem cells with biomaterial-based matrices. Apart from an earlier trial that confirmed that autologous epidermal equivalents engineered from outer root sheath KCs (EpiDexÔ) were safe and as effective as a standard mesh graft in the treatment of recalcitrant vascular leg ulcers, 66 new skin analogues have not reached clinical trials/case studies. However, artificial dermis with non-cultured autologous hASCs and exogenous basic fibroblast growth factor (bFGF) showed a high degree of success in the healing of patients who suffered chronic radiation injuries.…”

Section: Clinical Cases and Trialsmentioning

confidence: 99%