An auto-inducible mechanism for ionic liquid resistance in microbial biofuel production

Ruegg, Thomas L.; Kim, Eun‐Mi; Simmons, Blake A.; Keasling, Jay D.; Singer, Steven W.; Lee, Taek Soon; Thelen, Michael P.

doi:10.1038/ncomms4490

Cited by 88 publications

(95 citation statements)

References 35 publications

(36 reference statements)

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“…Moreover, due to the lack of well-mixed conditions in high solid systems, the mechanisms and pathways that microorganisms use to tolerate IL may be different between aqueous and high solid systems . Although IL tolerant mechanisms have not been fully explored, a few studies have applied transcriptomic analyses to begin to elucidate the molecular mechanisms of IL tolerance , and have successfully demonstrated an engineered IL-tolerant microorganism with potential applications in converting IL treated biomass into advanced biofuels (Bokinsky et al 2011;Dickinson et al 2016;Frederix et al 2014;Ruegg et al 2014). These recent studies demonstrate the potential opportunities for discovering genes from environmental communities that confer IL tolerance, and engineering industrial microorganisms for bioprocesses that include IL pretreatment.…”

Section: Discussionmentioning

confidence: 99%

“…Growth in each well is measured in Omnilog (OL) units, which calculate the change in tetrazolium redox dye color intensity attributed to dye reduction during cell respiration (Bochner and Savageau 1977;Khudyakov et al 2012). A few studies have utilized the OmniLog phenotypic microarray to screen for IL-tolerance Ruegg et al 2014). For instance, in the study by Khudyakov et al, the assay was used in the empty plate mode to measure the growth of Enterobacter lignolyticus strain SCF1 in the presence of a range of [C 2 C 1 Im]Cl concentrations throughout a culture period of 72 h .…”

Section: Discovering and Screening For Il Tolerant Microorganisms Relmentioning

confidence: 99%

“…Unlike constitutive expression systems, which continually express the gene of interest, a dynamic expression system adjusts to the reaction environment (Holtz and Keasling 2010;Ruegg et al 2014). Such a dynamic system can be developed through a transcriptional regulator that is induced by the efflux pump substrate to increase expression of the pump (Grkovic et al 2001;Harrison and Dunlop 2012;Ruegg et al 2014).…”

Section: Engineering Il Tolerance In Bacteriamentioning

confidence: 99%

“…Such a dynamic system can be developed through a transcriptional regulator that is induced by the efflux pump substrate to increase expression of the pump (Grkovic et al 2001;Harrison and Dunlop 2012;Ruegg et al 2014). The study by Khudyakov et al revealed that [C 2 C 1 Im]Cl caused the differential expression of several transporter genes, including the highly expressed mfs1 (E. lignolyticus locus tag Entcl_2352), a gene of unknown function predicted to be an MFS membrane transporter .…”

Section: Engineering Il Tolerance In Bacteriamentioning

confidence: 99%

See 3 more Smart Citations

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs not only inhibit enzyme activities, but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes.While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. In this article, we review recent progress on discovering IL tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discovering and Screening For Il Tolerant Microorganisms Relmentioning

confidence: 99%

Section: Engineering Il Tolerance In Bacteriamentioning

confidence: 99%

Section: Engineering Il Tolerance In Bacteriamentioning

confidence: 99%

See 2 more Smart Citations

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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“…3,19,28,[30][31][32][33][34][35] Acid catalyst has been used previously to hydrolyze polysaccharides to monosaccharides following IL pretreatment, which could potentially provide an enzyme-free process with signicant reduction of the processing time and material cost. 3,32,36 It was reported that over 80% glucose and 90% xylose were released with the integrated IL-acidolysis process at lab scale.…”

mentioning

confidence: 99%

Conversion of cellulose rich municipal solid waste blends using ionic liquids: feedstock convertibility and process scale-up

Liang

et al. 2017

RSC Adv.

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Sixteen cellulose rich municipal solid waste (MSW) blends were developed and screened using an acidassisted ionic liquid (IL) deconstruction process. Corn stover and switchgrass were chosen to represent herbaceous feedstocks; non-recyclable paper (NRP) and grass clippings (GC) collected from households were chosen as MSW candidates given their abundance in municipal waste streams. The most promising MSW blend: corn stover/non-recyclable paper (CS/NRP) at 80/20 ratio was identified in milliliter-scale screening based on the sugar yield, feedstock cost, and availability. A successful scale-up (600-fold) of the IL-acidolysis process on the identified CS/NRP blend has been achieved. The sugar and lignin streams were recovered and characterized. Mass and material energy flows of the optimized process were presented. Feedstock cost for MSW blends was also discussed. Results suggest the promising potential of using MSW as a feedstock blending agent for biorefineries while maintaining sufficient performance and low feedstock cost. The bench scale (6 L) study is an essential step in demonstrating the scalability of this IL technology.

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An Overview on the Toxicological Properties of Ionic Liquids toward Microorganisms

Sivapragasam

Moniruzzaman

Goto

2020

Biotechnology Journal

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Ionic liquids (ILs), a class of materials with unique physicochemical properties, have been used extensively in the fields of chemical engineering, biotechnology, material sciences, pharmaceutics, and many others. Because ILs are very polar by nature, they can migrate into the environment with the possibility of inclusion in the food chain and bioaccumulation in living organisms. However, the chemical natures of ILs are not quintessentially biocompatible. Therefore, the practical uses of ILs must be preceded by suitable toxicological assessments. Among different methods, the use of microorganisms to evaluate IL toxicity provides many advantages including short generation time, rapid growth, and environmental and industrial relevance. This article reviews the recent research progress on the toxicological properties of ILs toward microorganisms and highlights the computational prediction of various toxicity models.

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An auto-inducible mechanism for ionic liquid resistance in microbial biofuel production

Cited by 88 publications

References 35 publications

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Conversion of cellulose rich municipal solid waste blends using ionic liquids: feedstock convertibility and process scale-up

An Overview on the Toxicological Properties of Ionic Liquids toward Microorganisms

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