An atypical Pseudomonas aeruginosa associated with cystic fibrosis of the pancreas

Doggett, Robert G.; Harrison, Gunyon M.; Stillwell, Richard; Wallis, Everett S.

doi:10.1016/s0022-3476(66)80152-x

Cited by 151 publications

(88 citation statements)

References 7 publications

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“…We chose a nonmucoid strain partly because we have investigated this strain (P455) extensively in our laboratory [4,23], and partly because the initial colonization of the respiratory mucosa is usually by nonmucoid strains, which become mucoid with chronic infection [24]. An organ culture with an air-mucosal interphase has a number of advantages.…”

Section: Discussionmentioning

confidence: 99%

Interaction of Pseudomonas aeruginosa with human respiratory mucosa in vitro

Tsang

Rutman²,

Tanaka³

et al. 1994

Eur Respir J

100

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I In nt te er ra ac ct ti io on n o of f P Ps se eu ud do om mo on na as s a ae er ru ug gi in no os sa a w wi it th h h hu um ma an n r re es sp pi ir ra at to or ry y m mu uc co os sa a i in n v vi it tr ro o Transmission electron microscopy (TEM) showed that uninfected organ cultures had normal ultrastructure. TEM of infected organ cultures at 8 h showed significant epithelial damage: 43.9±10% of cells extruding from the epithelial surface, 17.7±3% of cells with loss of cilia, 32.9±10.2% of cells with mitochondrial damage, and 11.6±3% of cells with cytoplasmic blebbing. P. aeruginosa only infrequently adhered to normal epithelium, but adhered to areas of epithelial damage and to basement membrane. Scanning electron microscopy (SEM) of organ cultures up to 2 h found P. aeruginosa only infrequently associated with mucus. SEM at 4 h revealed P. aeruginosa predominantly associated with mucus and extruded damaged epithelial cells, but also occasionally associated with cilia, and very occasionally with unciliated cells. SEM also revealed loss of epithelial tight junctions in P. aeruginosa infected organ cultures, and P. aeruginosa were frequently seen in the gaps between epithelial cells. An extracellular matrix, possibly of bacterial origin, was seen bridging the space between bacteria and cell surface.We conclude that P. aeruginosa infection of this organ culture caused tissue damage and that P. aeruginosa preferentially adhered to mucus, damaged epithelium and basement membrane.

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Section: Discussionmentioning

confidence: 99%

Interaction of Pseudomonas aeruginosa with human respiratory mucosa in vitro

Tsang

Rutman²,

Tanaka³

et al. 1994

Eur Respir J

100

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show abstract

“…Experimental pneumonia was established by tracheobronchial instillation of suspensions of microscopic agar beads, which were impregnated with viable P. aeruginosa. After 4 wk of infection, the geometric mean (reciprocal) passive hemagglutinating Pseudomonas antibody titer was 185±1.3, and lungs contained 16.8 ±4 x 103 colony-forming units Pseudomonas/ml of lung homogenate. Pseudomonas immunization, given prior to a 4-wk infection, resulted in significantly higher passive hemagglutinating titers (474±1.4; P < 0.05), lower numbers of viable Pseudomonas in lung tissues (2.4±0.6 x 103; P < 0.01), and reduced histopathology in lungs.…”

Section: Abstract Chronic Respiratory Infection Withmentioning

confidence: 99%

“…Such complexes, if deposited in lung tissues, might predispose to hypersensitivity lung disease (12,13), and further immune stimulation by vaccine would be contraindicated in that setting. Fourth, the peculiar mucoid slime coating frequently associated with Pseudomonas in the CF lung (1)(2)(3)(4)(5)(6)(7)16), may act as an antiphagocytic and antiopsonic substance (17,18), rendering antibodies to somatic antigen less effective. Finally, the unpleasant side effects associated with lipopolysaccharide bacterial vaccine (8), have impeded rapid acceptance of such vaccines by clinicians, even for pilot investigations.…”

Section: Abstract Chronic Respiratory Infection Withmentioning

confidence: 99%

Active Immunization with Lipopolysaccharide Pseudomonas Antigen for Chronic Pseudomonas Bronchopneumonia in Guinea Pigs

Pennington¹,

Hickey²,

Blackwood³

et al. 1981

J. Clin. Invest.

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“…Most P. aeruginosa isolates from CF lung infection elaborate an extensive exopolysaccharide at their surface (Doggett et al, 1966) which often is not stable upon culture in vitro. Studies by electronmicroscopy of bronc hoscopy and postmortem lung specimens from CF patients infected with P. aeruginosa have revealed extensive formation of microcolonies embedded in exopolysaccharide (Lam et al, 1980).…”

Section: Introductionmentioning

confidence: 99%