An attempt to differentiate HPLC-ICP-MS selenium speciation in natural and selenised Agaricus mushrooms using different species extraction procedures

Abstract

Total determination and speciation analysis of Se in commercial and selenised Agaricus mushrooms have been performed to investigate the Se species naturally occurring in non-enriched mushrooms as well as those present in specimens grown in a Se-enriched medium. Mushroom aqueous and enzymatic extracts have been analysed by three complementary chromatographic separation mechanisms (size-exclusion, anion-exchange and reversed-phase) coupled to an inductively coupled plasma mass spectrometer with an octopole reaction system. Post-column isotope dilution analysis has been used on-line with the separations for quantification of the Se species eluted. The 78Se-to-77Se isotope ratio was monitored after adequate corrections for both total determinations and Se species quantitative speciation. The results showed marked differences not only in total Se contents but also in Se species found in the two types of Agaricus mushrooms investigated. Selenomethionine was detected in both of them (free in commercial mushrooms and incorporated into proteins in selenised ones) together with a number of unknown selenocompounds.

Distribution of iron, cobalt, zinc and selenium in macrofungi

“…Gergely et al (2006) found selenocystine, selenomethionine, methylselenocysteine and inorganic selenium in Lentinula edodes and Agaricus bisporus. Moreover, a number of unknown selenocompounds, all of them corresponding to low molecular weight species, were also detected (Huerta et al 2005(Huerta et al , 2006. According to the studies on Se bioavailability (Mutanen 1986;Chansler et al 1986), selenium of macrofungi is poorly available for the needs of a human body.…”

Notice of Retraction: Determination of Selenium Speciation in Tea Samples by HPLC-DRC-ICP-MS

“…Selenium is an important trace element for human health as being incorporated in proteins, shows anti carcinogenic role and prevents heavy metal toxic effects [1], but it is also a potential toxicant [2]. As bioavailability and absorption strongly depend on chemical form in which the element is present, rapid, accurate and precise analytical methodologies for the qualitative and quantitative speciation analysis of selenium in foodstuffs are now becoming more and more necessary [3].…”

Selenium Bioaccumulation in Shiitake Mushrooms: A Nutritional Alternative Source of this Element

“…pinicola , B. aestivalis , and Xerocomus badius (Kalač and Svoboda 2000). In commercial mushrooms, selenomethionine can be detected in its free form, and it can also incorporated into proteins in selenized mushrooms, together with a number of unknown selenocompounds (Huerta and others 2006; Silva and others 2010; Cremades and others 2012). …”