An ATP fluorescent chemosensor based on a Zn(ii)-complexed dipicolylaminereceptor coupled with a naphthalimidechromophore

Abstract

A fluorescent naphthalimide chemosensor for ATP bearing a dipicolylamine group complexed with a Zn(II) metal as a receptor moiety was synthesized and its sensing properties regarding ATP and other related phosphate species were evaluated.

Surface-functionalized fluorescent silica nanoparticles for the detection of ATP

“…Such p-p stacking effects have previously been made responsible for the differences in interaction between closely related nucleotides (which differed only in the nucleic base) and acridine bearing a Zn(II)-cyclen unit. 7 It is assumed to be the main reason for the observed selectivity towards ATP (and also ADP, as reported previously) 4 when compared with the other nucleotides having different bases (see Fig. S2 in ESIw).…”

“…4 This loss of sensitivity can be explained by the interaction mechanism between the indicator dye and ATP. It is assumed that this interaction is not only due to the binding of the phosphate groups in ATP to the Zn(II)-DPA receptor unit, but also due to p-p stacking effects between the adenine moiety of ATP and the naphthalimide fluorophore in the indicator dye.…”

“…With this in mind, our group developed a fluorescent indicator for ATP, 4Á Zn, based on naphthalimide as the fluorophore and a Zn(II) metal cation complexed with a dipicolylamine (DPA) moiety as the receptor. 4 Apart from its sensitivity to ATP, this indicator provides very favorable optical properties, such as high quantum yields and a large Stokes' shift.The immobilization of fluorescent indicator dyes onto nanoparticles is of significant importance since plain organic dyes are often toxic to cells. Moreover, many indicator dyes only show changes in fluorescence intensity making them unreliable for quantitative measurements of analyte concentration, since effects such as dye aggregation or fluctuations in the light source intensity may influence their fluorescence.…”

“…With this in mind, our group developed a fluorescent indicator for ATP, 4Á Zn, based on naphthalimide as the fluorophore and a Zn(II) metal cation complexed with a dipicolylamine (DPA) moiety as the receptor. 4 Apart from its sensitivity to ATP, this indicator provides very favorable optical properties, such as high quantum yields and a large Stokes' shift.…”

Pillararene-based fluorescent indicator displacement assay for the selective recognition of ATP

“…Since the nucleotides, especially ATP is pivotal in living systems [38],numerous fluorescent chemosensors have been developed for their detection [39][40][41][42][43], involving direct sensing [44,45], cation-based recognition units [46][47][48][49][50][51][52] and also some indicator displacement assays [53][54][55][56][57].…”