An ATP Binding Cassette Transporter Is Required for Cuticular Wax Deposition and Desiccation Tolerance in the Moss <i>Physcomitrella patens</i>

Buda, Gregory J.; Barnes, William J.; Fich, Eric A.; Park, Sung Jin; Yeats, Trevor H.; Zhao, Lingxia; Domozych, David S.; Rose, Jocelyn K. C.

doi:10.1105/tpc.113.117648

Cited by 102 publications

(93 citation statements)

References 60 publications

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“…S15) showed a clear enrichment in term function associated with the cell wall and cell periphery term. For example, the absence of gametophores in Ddek1 at 14 d reflects the reduction of transcript coding for P. patens ATP binding cassette subfamily G (ABCG) transporter PpABCG28 (Pp1s198_152V6), a protein associated with cuticle transport present only in gametophores (Buda et al, 2013). Similarly, NO APICAL MERISTEM, ARABIDOPSIS TRANSCRIPTION ACTIVATION FACTOR and CUP-SHAPED COTYLEDON transcription factor PpVNS1 and PpVNS5 (Pp1s182_37V6.1 and Pp1s223_ 12V6.1, respectively; Supplemental Fig.…”

Section: Ppdek1 Deletion Alters Gene Expression Prior To Bud Formationmentioning

confidence: 99%

Genetic Analysis ofDEFECTIVE KERNEL1Loop Function in Three-Dimensional Body Patterning inPhyscomitrella patens

Demko

Perroud

Johansen³

et al. 2014

Plant Physiology

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DEFECTIVE KERNEL1 (DEK1) of higher plants plays an essential role in position-dependent signaling and consists of a large transmembrane domain (MEM) linked to a protease catalytic domain and a regulatory domain. Here, we show that the postulated sensory Loop of the MEM domain plays an important role in the developmental regulation of DEK1 activity in the moss Physcomitrella patens. Compared with P. patens lacking DEK1 (Δdek1), the dek1Δloop mutant correctly positions the division plane in the bud apical cell. In contrast with an early developmental arrest of Δdek1 buds, dek1Δloop develops aberrant gametophores lacking expanded phyllids resulting from misregulation of mitotic activity. In contrast with the highly conserved sequence of the protease catalytic domain, the Loop is highly variable in land plants. Functionally, the sequence from Marchantia polymorpha fully complements the dek1Δloop phenotype, whereas sequences from maize (Zea mays) and Arabidopsis (Arabidopsis thaliana) give phenotypes with retarded growth and affected phyllid development. Bioinformatic analysis identifies MEM as a member of the Major Facilitator Superfamily, membrane transporters reacting to stimuli from the external environment. Transcriptome analysis comparing wild-type and Δdek1 tissues identifies an effect on two groups of transcripts connected to dek1 mutant phenotypes: transcripts related to cell wall remodeling and regulation of the AINTEGUMENTA, PLETHORA, and BABY BOOM2 (APB2) and APB3 transcription factors known to regulate bud initiation. Finally, sequence data support the hypothesis that the advanced charophyte algae that evolved into ancestral land plants lost cytosolic calpains, retaining DEK1 as the sole calpain in the evolving land plant lineage.

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Section: Ppdek1 Deletion Alters Gene Expression Prior To Bud Formationmentioning

confidence: 99%

Genetic Analysis ofDEFECTIVE KERNEL1Loop Function in Three-Dimensional Body Patterning inPhyscomitrella patens

Demko

Perroud

Johansen³

et al. 2014

Plant Physiology

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“…Thus, a waxy cuticle with a similar composition protects mosses and seed plants from dehydration, with the plasma membrane transporter involved in its formation being functionally conserved during evolution (Buda et al 2013). Reproductive propagules in land plants, i.e., spores and pollen, are protected by a tough sporopollenin wall.…”

Section: Moss Is Amenable To Fundamental Research In Cell Biologymentioning

confidence: 99%

Can mosses serve as model organisms for forest research?

Müller

Gütle

Jacquot

et al. 2016

Annals of Forest Science

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& Key message Based on their impact on many ecosystems, we review the relevance of mosses in research regarding stress tolerance, metabolism, and cell biology. We introduce the potential use of mosses as complementary model systems in molecular forest research, with an emphasis on the most developed model moss Physcomitrella patens. & Context and aims Mosses are important components of several ecosystems. The moss P. patens is a well-established nonvascular model plant with a high amenability to molecular biology techniques and was designated as a JGI plant flagship genome. In this review, we will provide an introduction to moss research and highlight the characteristics of P. patens and other mosses as a potential complementary model system for forest research. & Methods Starting with an introduction into general moss biology, we summarize the knowledge about moss physiology and differences to seed plants. We provide an overview of the current research areas utilizing mosses, pinpointing potential links to tree biology. To complement literature review, we discuss moss advantages and available resources regarding molecular biology techniques. & Results and conclusion During the last decade, many fundamental processes and cell mechanisms have been studied in mosses and seed plants, increasing our knowledge of plant evolution. Additionally, moss-specific mechanisms of stress tolerance are under investigation to understand their resilience in ecosystems. Thus, using the advantages of model mosses such as P. patens is of high interest for various research approaches, including stress tolerance, organelle biology, cell polarity, and secondary metabolism.

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“…The capability to synthesize and secrete lipidic substances evolved in early land plants with the need for a protective coating against transpiration, the cutin layer [77,78]. Conceivably, AM-competent plant species evolved a parallel cutin-related pathway (involving RAM2 and STR/STR2) which produced cutin-like monomers that were used by early AM fungi as a substrate in addition to exuded sugars.…”

Section: The Bread-and-butter Hypothesismentioning

confidence: 99%

Diet of Arbuscular Mycorrhizal Fungi: Bread and Butter?

Rich

Nouri

Courty

et al. 2017

Trends in Plant Science

150

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Most plants entertain mutualistic interactions known as arbuscular mycorrhiza (AM) with soil fungi (Glomeromycota) which provide them with mineral nutrients in exchange for reduced carbon from the plant. Mycorrhizal roots represent strong carbon sinks in which hexoses are transferred from the plant host to the fungus. However, most of the carbon in AM fungi is stored in the form of lipids. The absence of the type I fatty acid synthase (FAS-I) complex from the AM fungal model species Rhizophagus irregularis suggests that lipids may also have a role in nutrition of the fungal partner. This hypothesis is supported by the concerted induction of host genes involved in lipid metabolism. We explore the possible roles of lipids in the light of recent literature on AM symbiosis. Carbohydrates in AM Fungal NutritionAM fungi are strictly biotrophic, in other words they depend on their host to complete their life cycle. Although the basis of biotrophy is poorly understood, it has been suggested that it is due to the dependency of AM fungi on some essential nutritional factor(s) from the host [1]. Direct uptake measurements revealed that intraradical mycelium (IRM) can take up carbohydrates, whereas extraradical mycelium (ERM) did not acquire significant amounts of carbohydrates [2,3]. Perhaps this is because AM fungal genes involved in nutrient uptake are expressed only in the host, which would explain their biotrophy. Tracer studies on mycorrhizal roots, and respirometric analysis on isolated intraradical hyphae have established glucose as a central substrate for AM fungi [3][4][5], and indeed a monosaccharide transporter has been identified in the fungal partner [6]. Mycorrhizal roots represent strong carbon sinks [2,4,7], suggesting that they attract sucrose from photosynthetic source tissues. Sucrose is thought to be cleaved in the vicinity of the fungus by invertases and sucrose synthase [8], resulting in monosaccharides (glucose and fructose) that are released to the fungus and taken up by its IRM [5,6]. However, in the fungal storage compartments -the spores and the vesicles -carbon is stored primarily in the form of lipids, with minor amounts of the glucose polymer glycogen. We discuss here salient issues relating to carbohydrate and lipid metabolism in AM fungi and their significance for fungal nutrition during symbiosis. Lipid Metabolism in AM Fungi -Open Questions and SurprisesAlthough the aforementioned carbon fluxes in AM fungi have been firmly established, several questions remain open. AM fungi store and transport most of their carbon in the form of lipids [9][10][11][12]. However, AM fungi cannot produce the basic fatty acid (FA) palmitate (C16) in the absence of their host (as shown in two distantly related species, Rhizophagus irregularis and Gigaspora rosea), while FA elongation and desaturation can occur independently of the plant [13]. This and similar results suggested that AM fungi can only synthesize C16 in the IRM [3,13]. Taken together, the available evidence suggests that AM fungi take up sugars (...

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An ATP Binding Cassette Transporter Is Required for Cuticular Wax Deposition and Desiccation Tolerance in the Moss Physcomitrella patens

Cited by 102 publications

References 60 publications

Genetic Analysis ofDEFECTIVE KERNEL1Loop Function in Three-Dimensional Body Patterning inPhyscomitrella patens

Genetic Analysis ofDEFECTIVE KERNEL1Loop Function in Three-Dimensional Body Patterning inPhyscomitrella patens

Can mosses serve as model organisms for forest research?

Diet of Arbuscular Mycorrhizal Fungi: Bread and Butter?

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