An ATL78-Like RING-H2 Finger Protein Confers Abiotic Stress Tolerance through Interacting with RAV2 and CSN5B in Tomato

Song, Jianwen; Xing, Yali; Munir, Sadia; Yu, Changlong; Song, Lulu; Li, Hanxia; Wang, Taotao; Ye, Zhibiao

doi:10.3389/fpls.2016.01305

Cited by 38 publications

(26 citation statements)

References 61 publications

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“…It is also possible that other zinc-finger proteins use a similar mechanism to promote abiotic stress tolerance. For instance, recent research from our colleague revealed that an ATL78-like RING-H2-finger protein (ShATL78L) can interact with CSN5B in tomato and that overexpressing this protein can enhance the tolerance of tomato to multiple abiotic stresses (Song et al, 2016).…”

Section: Zinc-finger Protein Functions As a New Regulator For Ascorbamentioning

confidence: 99%

The C2H2 zinc‐finger protein SlZF3 regulates AsA synthesis and salt tolerance by interacting with CSN5B

Liu

Chu

Luo

et al. 2017

Plant Biotechnology Journal

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SummaryAbiotic stresses are a major cause of crop loss. Ascorbic acid (AsA) promotes stress tolerance by scavenging reactive oxygen species (ROS), which accumulate when plants experience abiotic stress. Although the biosynthesis and metabolism of AsA are well established, the genes that regulate these pathways remain largely unexplored. Here, we report on a novel regulatory gene from tomato (Solanum lycopersicum) named SlZF3 that encodes a Cys2/His2‐type zinc‐finger protein with an EAR repression domain. The expression of SlZF3 was rapidly induced by NaCl treatments. The overexpression of SlZF3 significantly increased the levels of AsA in tomato and Arabidopsis. Consequently, the AsA‐mediated ROS‐scavenging capacity of the SlZF3‐overexpressing plants was increased, which enhanced the salt tolerance of these plants. Protein–protein interaction assays demonstrated that SlZF3 directly binds CSN5B, a key component of the COP9 signalosome. This interaction inhibited the binding of CSN5B to VTC1, a GDP‐mannose pyrophosphorylase that contributes to AsA biosynthesis. We found that the EAR domain promoted the stability of SlZF3 but was not required for the interaction between SlZF3 and CSN5B. Our findings indicate that SlZF3 simultaneously promotes the accumulation of AsA and enhances plant salt‐stress tolerance.

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Section: Zinc-finger Protein Functions As a New Regulator For Ascorbamentioning

confidence: 99%

The C2H2 zinc‐finger protein SlZF3 regulates AsA synthesis and salt tolerance by interacting with CSN5B

Liu

Chu

Luo

et al. 2017

Plant Biotechnology Journal

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“…Actually, LOC107817253 and LOC107782318 are both RING‐H2 finger protein ATL2‐like, which has been reported to be associated with plant resistance in tomato ( Lycopersicon esculentum Mill.) (Song et al., 2016) and rice (Park, Choi, Kim, & Jang, 2019). Therefore, we speculated that LOC107815159 might respond to low NO 3 − stress by regulating the expression of RING‐H2 finger protein.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of tobacco root response to different concentrations of nitrate

et al. 2020

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We aimed to explore genes and transcription factors (TFs) in tobacco (Nicotiana tabacum L.) that were closely related to responses to different concentrations of nitrate (NO 3 − ) in tobacco root. Cultivar K326 was used in this study. The RNA sequence data generated from K326 treated with high or low concentration of NO 3 − supplied at different time points (0, 6, 12, and 24 h) were analyzed to determine the transcriptome response of tobacco root to NO 3 − supply. The expression of numerous genes was altered after high and low NO 3 − treatment. Further analysis revealed 1981 and 3573 differentially expressed genes (DEGs) from low and high NO 3 − treatment groups, respectively. Comparative analysis identified 567 low-NO 3 −specific, 2159 high-NO 3 − -specific, and 1414 commonly expressed genes. Combining short time-series expression miner (STEM) and weighed gene co-expression network analysis (WGCNA) results, 136 and 416 DEGs were identified from low and high NO 3 − groups, respectively, among which, 12 were TFs. In the constructed protein−protein interaction (PPI) network, three DEGs in high NO 3 − group (LOC107807186, LOC107792457, and LOC107786314) exhibited highest degrees. Within low NO 3 − group, LOC107798206, LOC107815159, and LOC107779796 had highest degrees. Among the 12 TFs, LOC107778750, LOC107810552, and LOC107804260 had the highest degrees. Our data provides valuable information about genes, especially the 12 TFs, which were induced by different concentrations of NO 3 − in tobacco root. These findings may be useful for identification of high or low NO 3 − treatment-induced genes and TFs, as well as for understanding potential gene regulation mechanisms in response to NO 3 − supply in tobacco.

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“…In this study, the expressions of a number of genes involved in the synthesis of these protective compounds have been found to be changed (Table 5). For example, the RING finger protein, besides the role as a ROS scavenger, was found to be also involved in the synthesis of proline, soluble carbohydrate, and osmotic regulation [48][49][50]. In the transgenic Arabidopsis plants with higher expression of the RING finger gene, the proline and sugar contents were increased and this…”

Section: Compatible Compounds Synthesis and Osmotic Regulationmentioning

confidence: 99%

Understanding the Physiological and Transcriptional Mechanism of Reproductive Stage Soybean in Response to Heat Stress

Xu¹,

Xia²,

Huang³

et al. 2019

Crop Breed Genet Genom.

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Understanding the physiological and molecular processes in response to heat in soybean is important to implement strategies to breed more heat tolerant soybean. In this study, physiological and RNA-Seq analysis were used to dissect the physiological alterations and molecular responses in the leaves of reproductive stage soybean grown at heat condition. Stomata conductance and transpiration rate were increased but photosynthesis and relative water content were reduced in response to heat. Among the three measured antioxidant enzymes, the activity of peroxidase was increased but no changes were detected for catalase and superoxide dismutase. A total of 633 annotated genes were found to be differentially expressed in heat stressed plants in which the expression levels of 417 genes were up-regulated and 216 were down-regulated. Most dramatic responses are related to flowering, oxidative stress, protein and mRNA folding and degradation, protective compounds synthesis, and ethylene biosynthesis and signaling. The diverse pathways identified in this study indicated that soybean employs complicated mechanisms to cope with heat. A few of the identified genes and pathways may be manipulated to improve heat resistance ability in soybean via either genetic engineering or molecular breeding method.

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An ATL78-Like RING-H2 Finger Protein Confers Abiotic Stress Tolerance through Interacting with RAV2 and CSN5B in Tomato

Cited by 38 publications

References 61 publications

The C2H2 zinc‐finger protein SlZF3 regulates AsA synthesis and salt tolerance by interacting with CSN5B

The C2H2 zinc‐finger protein SlZF3 regulates AsA synthesis and salt tolerance by interacting with CSN5B

Transcriptome analysis of tobacco root response to different concentrations of nitrate

Understanding the Physiological and Transcriptional Mechanism of Reproductive Stage Soybean in Response to Heat Stress

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