An assessment of the toxicity of polypropylene microplastics in human derived cells

Hwang, Jang‐Sun; Choi, Daheui; Han, Seora; Choi, Jonghoon; Hong, Jinkee

doi:10.1016/j.scitotenv.2019.05.071

Cited by 446 publications

(253 citation statements)

References 98 publications

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“…The literature already includes several reports of ROS generation and the subsequent toxic effects such as genetic, mitochondrial and DNA damages in toxicity studies with engineered metal (oxide) nanoparticles [44,45]. A number of studies have also, in agreement with our ndings, reported the induction of oxidative stress caused by exposure to small pieces of plastics [46].…”

Section: Discussionsupporting

confidence: 80%

Physiological stress response of the Wistar albino rats orally exposed to polystyrene nanoparticles

Babaei

Rafiee

Khodagholi

et al. 2020

Preprint

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Background Few studies have examined nano-sized plastic particulates (NPs) exposure in relation to oxidative stress and biochemical responses in rodents, commonly used for toxicity evaluations on which to base risk assessment for humans.Methods Here we explored possible oxidative stress and biochemical responses of five weeks oral exposure to polystyrene (PS) nanoparticles (1, 3, 6 and 10 mg/kg body weight per day) in male rats. We used variance analysis and variance explained statistic eta-squared (𝜂2) to estimate the strength of relationships worked out. The whole body scanning further provided insight into the bio-distribution of nanoplastics upon oral exposure.Results Results demonstrated the accumulation of PS-NPs through whole body and also a dose-dependent increase in the production of reactive oxygen species (ROS). Significant alterations in antioxidant responses including serum levels of catalase, superoxide dismutase (SOD), and total glutathione content were noticed, pointing towards a perturbation of redox state induced by the exposure conditions. Acetylcholinesterase level in highest dose group was about 40 percent lower than those in control group. Biochemical parameters viz. glucose, cortisol, lipase, lactate, lactate dehydrogenase (LDH), alkaline phosphatase, gamma-glutamyl transpeptidase (GGT), triglycerides, and urea showed a significant increase, while total protein, albumin and globulin levels showed an appreciable decline.Conclusion The pattern of associations noticed with AChE activity and biochemical responses in our study suggests the possibility that a neurobehavioral effect or dysfunctions in energy metabolism, or both, may be the potential mode of action, possibly through stress response as well as liver function. Perturbations of creatinine and uric acid levels are indeed plausible biological explanations for the association with kidney dysfunction. Although we provided a new scientific clue for exploring the biological effects of plastics nanoparticles, the results warrant additional research with a larger sample size. The suggested potential mechanisms also remains to be investigated.

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Section: Discussionsupporting

confidence: 80%

Physiological stress response of the Wistar albino rats orally exposed to polystyrene nanoparticles

Babaei

Rafiee

Khodagholi

et al. 2020

Preprint

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“…Recent immense attention paid on the cytotoxicity of micro/nano-plastics in human and animal models evidenced that plastic particles produce oxidative stress in cells at low concentration and cytotoxic at higher concentrations [68,69]. In the present study, we have provided insights into the concentrationdependent regulatory, cytoprotective, and cytotoxic effects in HaCaT cells produced by smaller plastic particles.…”

Section: The Cytoprotective Response Of Nps Exposed Cellsmentioning

confidence: 68%

Prospects on the nano-plastic particles internalization and induction of cellular response in human keratinocytes

Gopinath

Twayana

Ravanan

et al. 2020

Preprint

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Background: Today cosmetic usage becomes customary in both sexes to improve their appearance and increase societal visibility. In this study, we have isolated nano-sized plastic particles ranging between 30 to 300 nm from the commercial face-scrubs and investigated its effects on keratinocytes. Results: Initially, nano-plastics adsorbed protein molecules and formed protein corona, thereby mimicked as protein aggregates, which then triggered the macropinocytosis activity. As a result, corrosion and degradation of plastic particles were observed. Concurrently, nano-plastics concentration-dependent cytotoxic, cytostatic, and cytoprotective activities were found in the keratinocytes. Additionally, a single dose of nano-plastics exposure for 48hrs resulted in the ROS mediated down-regulation of cell growth and proliferation inhibition followed by autophagy, finally, premature aging in HaCaT cells in 24 and 72 hrs of post-internalization, respectively. Conclusion: At the outset, this work provides insights into the nano-plastics concentration-dependent regulatory, cytoprotective, and cytotoxic effects in HaCaT cells. Further studies required to identify the detailed mechanisms of NPs toxicity on cells and the cytoprotective effect in cells at the molecular level.

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“…Wu and colleagues also worked with Caco-2 cells, and reported that, while a low degree of toxicity was observed for PS NPs (at 0.1 and 5 µm), they induced mitochondrial depolarization and inhibited the activity of the toxicant efflux pump, ATP-binding cassette (ABC) transporter, with the latter resulting in increased arsenic toxicity [117]. Hwang et al worked with a number of cell types of human and mouse origin, and documented cytotoxicity associated with 20 µm PP MPs at high concentrations and ROS induction [118]. The MPs also measurably induced pro-inflammatory cytokines IL-6 and TNF-α from human peripheral blood mononuclear cells (PBMCs), and increased histamine release from mast cell lines [118].…”

Section: Toxicity Of Mps/nps In Human Cellsmentioning

confidence: 99%

“…Hwang et al worked with a number of cell types of human and mouse origin, and documented cytotoxicity associated with 20 µm PP MPs at high concentrations and ROS induction [118]. The MPs also measurably induced pro-inflammatory cytokines IL-6 and TNF-α from human peripheral blood mononuclear cells (PBMCs), and increased histamine release from mast cell lines [118]. Poma and colleagues found that 100 nm PS NPs stimulated ROS production and induced genotoxic stress and DNA damage as measured with the cytokinesis-block micronucleus (CBMN) assay [119].…”

Section: Toxicity Of Mps/nps In Human Cellsmentioning

confidence: 99%

Toxicity of Microplastics and Nanoplastics in Mammalian Systems

Yong

Valiyaveettil

Tang

2020

IJERPH

523

280

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Fragmented or otherwise miniaturized plastic materials in the form of micro- or nanoplastics have been of nagging environmental concern. Perturbation of organismal physiology and behavior by micro- and nanoplastics have been widely documented for marine invertebrates. Some of these effects are also manifested by larger marine vertebrates such as fishes. More recently, possible effects of micro- and nanoplastics on mammalian gut microbiota as well as host cellular and metabolic toxicity have been reported in mouse models. Human exposure to micro- and nanoplastics occurs largely through ingestion, as these are found in food or derived from food packaging, but also in a less well-defined manner though inhalation. The pathophysiological consequences of acute and chronic micro- and nanoplastics exposure in the mammalian system, particularly humans, are yet unclear. In this review, we focus on the recent findings related to the potential toxicity and detrimental effects of micro- and nanoplastics as demonstrated in mouse models as well as human cell lines. The prevailing data suggest that micro- and nanoplastics accumulation in mammalian and human tissues would likely have negative, yet unclear long-term consequences. There is a need for cellular and systemic toxicity due to micro- and nanoplastics to be better illuminated, and the underlying mechanisms defined by further work.

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An assessment of the toxicity of polypropylene microplastics in human derived cells

Cited by 446 publications

References 98 publications

Physiological stress response of the Wistar albino rats orally exposed to polystyrene nanoparticles

Physiological stress response of the Wistar albino rats orally exposed to polystyrene nanoparticles

Prospects on the nano-plastic particles internalization and induction of cellular response in human keratinocytes

Toxicity of Microplastics and Nanoplastics in Mammalian Systems

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