An assessment of the suitability of bromocresol green for the determination of serum albumin

Webster, David; Bignell, A.H.C.; Attwood, E.C.

doi:10.1016/0009-8981(74)90357-x

Cited by 93 publications

(34 citation statements)

References 10 publications

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“…When comparing the mean values of all methods for BCG (excluding VITROS) to BCP, we observed that the BCP methods were 0.21 g/dL lower than were the BCG methods (Table 1). Other reports [12][13][14][15] have documented that albumin values for BCP methods are 0.3 to 0.5 g/dL lower than are those obtained by BCG methods. When comparing our results to the respective designated comparison methods, we observed positive biases of 0 to 0.3 g/dL for BCG methods (excluding VITROS) and larger positive biases of 0.3 to 0.5 for BCP methods.…”

Section: Comment Albuminmentioning

confidence: 90%

Laboratory Performance in Albumin and Total Protein Measurement Using a Commutable Specimen: Results of a College of American Pathologists Study

Miller

Doumas

2013

Archives of Pathology &Amp; Laboratory Medicine

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Context.-Discrepant results for serum constituents were observed among peer groups in the College of American Pathologists Comprehensive Chemistry Survey.Objectives.-To assess the performance of serum albumin and total protein measurement procedures and to evaluate the commutability of the conventional survey specimens.Design.-A fresh frozen, off-the-clot serum sample was included along with 4 conventional survey specimens. The fresh frozen, off-the-clot serum sample was prepared in a manner expected to confer commutability with native clinical samples.Results.-For the fresh frozen, off-the-clot serum sample, the mean values for 17 peer-groups were À0.07 to 0.32 g/dL from the bromocresol green albumin designated comparison method, whereas 4 VITROS (Ortho Clinical Diagnostics, Rochester, New York) peer groups differed by À0.29 to À0.37 g/dL (15 of 21 differences [71%] had P , .001). For bromocresol purple albumin methods, the mean differences from the designated comparison method from 8 peer groups were 0.25 to 0.47 g/dL (all had P , .001). For total protein methods, 23 peer group mean values were À0.07 to 0.15 g/dL from the reference measurement procedure (12 of 24 [50%] had P , .001). The Beckman (Fullerton, California) Synchron LX20 had a bias of À0.30 g/dL (P ,.001). The commutability of the conventional specimens was acceptable for 23 of 24 bromocresol green method-material combinations (96%) and 13 of 16 bromocresol purple albumin method-material combinations (81%). All (100%) of the 36 method-material combinations had acceptable commutability for total protein.Conclusions.-One (2.2%) of the instrument systems (Synchron) using bromocresol green and none (0%) of the instrument systems using bromocresol purple had satisfactory total-error performance for albumin measurement. Differences in results between bromocresol green and bromocresol purple methods precluded using common reference intervals for interpreting results for serum albumin. Eight of 9 instrument systems (86.5%) had satisfactory total-error performance for total protein measurement.

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Section: Comment Albuminmentioning

confidence: 90%

Laboratory Performance in Albumin and Total Protein Measurement Using a Commutable Specimen: Results of a College of American Pathologists Study

Miller

Doumas

2013

Archives of Pathology &Amp; Laboratory Medicine

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“…A albumina sérica pré-diálise foi mensurada pelo mé-todo de verde de bromecrosol (23). A desnutrição foi considerada quando os valores de referência dessa proteína foram inferiores a 4,0g/dL, segundo K/DOQI (14), e 3,8g/dL, proposto pelo Comitê da Internacional Society of Renal Nutrition and Metabolism (ISRNM) (24).…”

Section: Materiais E Métodosunclassified

Análise de diferentes métodos de avaliação do estado nutricional de pacientes em hemodiálise

Ribeiro¹,

Araújo²,

Cunha³

et al. 2015

Rev Cuid

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RESUMENIntroducción: El paciente en diálisis debe ser evaluado por la nutrición y mantener seguimiento regular desde el inicio del tratamiento para así tener las mejores respuestas de intervenciones y dieta. Objetivo: Comparar la concordancia de la clasificación del estado nutricional de los pacientes con enfermedad renal crónica en hemodiálisis por medio de diferentes evaluaciones clínicas, antropométricas y parámetros bioquímicos. Materiales y Métodos: Estudio transversal con 90 pacientes de una clínica de diálisis, en la ciudad de Belo Horizonte, Brasil en 2010. Se recogieron los datos socioeconómicos, nutricionales, clínicos, bioquímicos y antropométricos. Resultados y Discusión: La frecuencia de desnutrición fue de 12,2% hasta 86,7% de acuerdo con diferentes parámetros de evaluación. En los análisis antropométricos, los hombres presentaran pliegues cutáneos más pequeños que las mujeres. Casi 40% de los pacientes tenían la clasificación de la desnutrición en los análisis de la circunferencia muscular del brazo, siendo aproximadamente 30% de hombres. Los valores promedios de índice de masa corporal se encontraban en el rango adecuado. Considerando la ingesta de energía y proteínas, 86,7% y 57,8% fueran clasificados como desnutridos, respectivamente. El valor promedio de albúmina fue 3,9 g / dl y la frecuencia de desnutrición varió 35,6% y 50%, según la referencia. Conclusiones: La alta frecuencia de pacientes con desnutrición de acuerdo con diferentes clasificaciones sugiere la necesidad de estandarización de indicadores, la observación cuidadosa y el seguimiento de las referencias utilizadas y también la necesidad de que los comités de evaluación en determinar nuevos puntos de corte para la mejor clasificación nutricional de esta población.

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“…It would seem unlikely that this is related to the tendency of BCG methods to overestimate albumin since a wide linear relationship has been observed between albumin measured by BCG and more specific methods.! ": 15 However, such a possibility cannot be excluded since the nature and the magnitude of the reported bias with BCG procedures is variable, particularly in pathological sera where concentrations of a andg lobulins may be increased. 16.…”

Section: Resultsmentioning

confidence: 99%

The Adjusted Serum Calcium Concept—A Reappraisal

1986

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The relationship between serum calcium and albumin was studied using a data base containing biochemical results on approximately 687 000 samples. To exclude patients with established bone, liver and kidney disease, calcium and albumin values had to be accompanied by additional results falling within the laboratory reference range for urea, phosphate, globulin, alkaline phosphatase and aspartate transaminase activities. The regression coefficients of calcium on albumin changed significantly following a change of instrumentation, even though the analytical principles of the methods remained the same. In addition, using standard laboratory methods, the relationship between calcium and albumin deviated from linearity at albumin concentrations less than 30 g/L. Care is needed in the selection and clinical application of factors for the adjustment of serum calcium for changes in the concentration of circulating albumin. Using a large data base, an albumin-related reference range for serum calcium may be calculated, eliminating the need for adjusting calcium values.

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An assessment of the suitability of bromocresol green for the determination of serum albumin

Cited by 93 publications

References 10 publications

Laboratory Performance in Albumin and Total Protein Measurement Using a Commutable Specimen: Results of a College of American Pathologists Study

Laboratory Performance in Albumin and Total Protein Measurement Using a Commutable Specimen: Results of a College of American Pathologists Study

Análise de diferentes métodos de avaliação do estado nutricional de pacientes em hemodiálise

The Adjusted Serum Calcium Concept—A Reappraisal

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