In order to manage nitrogen (N) and phosphorous (P) through organic fertilization in aquaculture ponds, it is important to understand the nutrient profile of different animal manures at various concentrations and the respective biological response. The rate of nutrient released from animal manure over time is a key factor in deciding the frequency and quantity of manure required to fertilize ponds. This study was conducted to elucidate the nutrient profile, and its efficiency in terms of primary productivity, of pig, cow, and buffalo manure, at different N:P concentrations in tank. Appropriate concentrations were maintained by using the minimum amount of inorganic fertilizer supplement. This paper also investigated the impact of lime-treated manure, a traditional practice in Vietnam, on primary production (phytoplankton abundance). The average nitrogen (N) and phosphorus (P) content was higher in pig manure (0.54% N and 0.173% P) compared to cow (0.31% N and 0.028% P) and buffalo (0.34% N and 0.013% P). The ammonia release rate increased with increase in N concentration. The average ammonia release rate (all concentrations, 1-2 ppm N) in the pig manure treatment was significantly higher than cow and buffalo manure treatments. Cow and buffalo manures were found to be similar in terms of ammonia re-Please note that this electronic prepublication galley may contain typographical errors and may be missing artwork, such as charts, photographs, etc. Pagination in this version will differ from the published version.