An assessment of Pythium spp. associated with soft rot disease of ginger (Zingiber officinale) in Queensland, Australia

Genetic variation in Pythium myriotylum based on SNP typing and development of a PCR-RFLP detection of isolates recovered from Pythium soft rot ginger

“…Sensitivity of the developed PCR‐RFLP were also assessed using genomic DNA extracts obtained directly from PSR ginger sampled from pot trials (Le et al . ).…”

“…G omez-Alp ızar et al (2011) successfully developed a specific PCR-RFLP of the ITS locus to detect and differentiate P. myriotylum isolates which were pathogenic to cocoyam from nonpathogenic P. myriotylum to cocoyam. Interestingly, regardless of a number of SNPs identified, the PSR P. myriotylum isolates only showed a certain level of host specificity to ginger (Le et al 2016), while cocoyam P. myriotylum isolates appeared totally host specificity, such isolates were identified by a unique SNP on the ITS2 region (G omez-Alp ızar et al 2011). Therefore, it will be of interest if future studies are to find any relationship between the genetic mutations in PSR P. myriotylum isolates and its high aggressiveness to ginger, as well as variable responses to environmental and ecological changes.…”

“…The RFLP products were electrophoresed and visualized as described above. Sensitivity of the developed PCR-RFLP were also assessed using genomic DNA extracts obtained directly from PSR ginger sampled from pot trials (Le et al 2016).…”

“…Similarly, P. myriotylum isolates obtained from Pythium soft rot (PSR) ginger in Australia showed pathogenicity over a wide range temperature from 20 to 35°C (Le et al . ).…”

“…P. myriotylum initially recovered from diseased cocoyam was able to quickly kill cocoyam plants at 24-28°C within a week in an inoculated pathogenicity assays (Tambong et al 1999;Perneel et al 2006). Similarly, P. myriotylum isolates obtained from Pythium soft rot (PSR) ginger in Australia showed pathogenicity over a wide range temperature from 20 to 35°C (Le et al 2016).…”