An assessment of correlation between serum asymmetric dimethylarginine and glycated haemoglobin in patients with type 2 diabetes mellitus

Abstract

Studies that investigated an association between asymmetric dimethylarginine (ADMA) and glycated haemoglobin (HbA1c) in type 2 diabetes mellitus (T2DM) have given discordant results. The aim of this study was to determine and compare serum ADMA concentration in patients with T2DM and healthy controls, and to assess correlation between ADMA and HbA1c in patients with T2DM. Serum ADMA concentration was determined by ELISA method with the use of ADMA ® - ELISA kit (DLD Diagnostics, Hamburg, Germany) and HbA1c levels were determined by an immunoturbidimetric method in 60 patients with T2DM and 60 healthy individuals matched for age and sex. Results have shown that mean serum ADMA concentration was significantly higher in T2DM patients (1.54±0.06 μmol/L) compared to mean serum ADMA concentration (0.62±0.02 μmol/L; p<0.0001) in healthy subjects. A significant, positive, correlation between serum ADMA concentration and HbA1c levels was observed (r=0.494; p<0.01) in T2DM patients. Our results suggest that there is an association between endothelial dysfunction and glycaemic control in type 2 diabetes mellitus. Possible explanation for obtained results may be oxidative stress that is increased in conditions of hyperglycaemia and it also promotes endothelial dysfunction. Larger, longitudinal studies are required that will evaluate relation between metabolic abnormalities and increased ADMA levels in patients with type 2 diabetes mellitus.

C - reactive protein and chitinase 3-like protein 1 as biomarkers of spatial redistribution of retinal blood vessels on digital retinal photography in patients with diabetic retinopathy

“…Some inflammatory biomarkers and risk factor for endothelial dysfunction such as C-reactive protein (CRP), tumor necrosis factor α (TNF-α), interleukin 18 (IL-1β) and serum asymmetric dimethylarginine (ADMA) have been the subjects of new studies [13][14][15][16][17][18][19]. Chitinase 3-like protein 1 is a novel biomarker of inflammation and tissue remodelation [20].…”

ADMA (asymmetric dimethylarginine) and angiogenic potential in patients with type 2 diabetes and prediabetes

“…28 Positive correlation of ADMA and HbA1c was also observed by Začiragić et al. 30 When conducting research on 270 patients with type 2 diabetes, Hsu et al. 31 did not find significant correlation of ADMA and HbA1c.…”

Diabetes-linked transcription factor HNF4α regulates metabolism of endogenous methylarginines and β-aminoisobutyric acid by controlling expression of alanine-glyoxylate aminotransferase 2

“…These observations raise the possibility that HNF4α-related diabetes may represent a metabolically-distinct subset of diabetes characterized by impairment in AGXT2 activity and a higher risk for development of cardiovascular complications due to the resulting metabolic abnormalities. Published data regarding the association between diabetes and circulating levels of methylarginines such as ADMA are contradictory 65 66 , perhaps because HNF4α-related diabetes represents only a subset of patients. Testing this hypothesis by assessing ADMA- and BAIB-related metabolic changes as well as the spectrum and prevalence of cardiovascular complications in the diabetic patients with HNF4A polymorphisms may lead to development of novel targeted therapeutic approaches for this subgroup of patients.…”