An Assessment of Antibacterial Activity of Four Endodontic Sealers on Enterococcus faecalis by a Direct Contact Test: An In Vitro Study

Abstract

Aim. To evaluate the antibacterial activity of four endodontic sealers on Enterococcus faecalis by a direct contact test. Material and Methods. Enterococcus faecalis was used as a test organism. Direct contact test which is based on measuring the effect of close contact between test bacteria and tested material on the kinetics of bacterial growth was performed to overcome the disadvantages of agar diffusion test. The sealers tested were zinc oxide eugenol-based sealer, glass-ionomer-based sealer, polydimethyl-siloxane-based sealer, and urethane dimethacrylate resin-based sealer. Data was collected by recording the optical density with the help of a spectrophotometer. Results. The sealers exhibited different inhibitory effects. The results obtained were subjected to statistical analysis by Kruskal Wallis analysis of variance and Dunn's multiple comparison test. Group comparison showed very highly significant difference between the groups. Conclusion. Zinc oxide eugenol-based sealer was the most effective and urethane dimethacrylate resin-based sealer was the least effective against Enterococcus faecalis, whereas glass-ionomer-based and polydimethyl-siloxane-based sealers were effective only for a short period. Inhibition of the bacterial growth is related to the direct contact of the microorganism with the sealer.

In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers

“…The current results evidenced that Endofill inhibited bacterial growth after 24 h, corroborating the outcomes of previous studies with ZOE cements. 23 As a matter of fact, eugenol in the sealer formulation has been described as a potent antimicrobial agent. 24 As demonstrated, AH Plus was effective against E. faecalis in ADT and in short-term DCT.…”

Anti Bacterial Effect of Different Adhesive Root Canal Sealer

“…E-faecalis is considered the most resistant species that can survive in the root canal system even after the endodontic treatment (7) It is advantageous for the endodontic sealers to have the property of bactericidal or at least the bacteriostatic activity. May help to eliminate the residual bacteria that was not eliminated during the chemo-mechanical preparation of the root canal system which can improve the success rate of the endodontic treatment (8,9) . This study was conducted to evaluate the antibacterial activity of glass ionomer based sealer compared to two resin based sealers using agar diffusion method (ADT).…”

“…This findings were comparable to Anumulaetal. (9) who stated that glass ionomer based sealer showed anti bacterial activity which may be related to the release of fluoride and the low pH in addition to the release of zinc ions. Another explanation may be related to the solubility of glass ionomer, which increased after 3 days and so increased its anti bacterial activity.…”

Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration

“…DCT, based on the turbidimetric determination of bacterial growth in 96-well microtiter plates, was used to evaluate the antibacterial activity of root canal sealing materials. 35,36 In this study, a modified DCT method was adopted to evaluate the antibacterial ability of the fibrous membranes. In brief, the test membranes (5×5 mm, n=4) were placed separately in 24-well microtiter plates (named as group A well plates).…”