2013
DOI: 10.1016/j.redox.2013.01.011
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An assay for the rate of removal of extracellular hydrogen peroxide by cells

Abstract: Cells have a wide range of capacities to remove extracellular hydrogen peroxide. At higher concentrations of extracellular H2O2 (micromolar) the rate of removal can be approximated by a rate equation that is first-order in the concentration of H2O2 and cell density. Here we present a method to determine the observed rate constant for the removal of extracellular H2O2 on a per cell basis. In the cells examined, when exposed to 20 μM H2O2, these rate constants (kcell) range from 0.46×10−12 s−1 cell−1 L for Mia-P… Show more

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Cited by 56 publications
(64 citation statements)
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“…The arbitrary units, relative fluorescence units (RFU), were based directly on fluorescence intensity. Intracellular ROS production was also evaluated using aminotriazole assay as described previously [37] using OxiSelect hydrogen peroxide/peroxidase assay kit following 12 hours of 10µM 4-HNE treatment according to manufacturer's instructions.…”
Section: Adipocyte Oxidative Stress Assay In Response To 4-hne Treatmentmentioning
confidence: 99%
“…The arbitrary units, relative fluorescence units (RFU), were based directly on fluorescence intensity. Intracellular ROS production was also evaluated using aminotriazole assay as described previously [37] using OxiSelect hydrogen peroxide/peroxidase assay kit following 12 hours of 10µM 4-HNE treatment according to manufacturer's instructions.…”
Section: Adipocyte Oxidative Stress Assay In Response To 4-hne Treatmentmentioning
confidence: 99%
“…(1) we model the diffusion of the peroxiredoxin forms and of the H 2 O 2 according to Fick's laws [49]. Per-cell H 2 O 2 removal rate constants determined by Wagner et al [50] allow to estimate the permeability constants in the range 10 − 10 m s…”
Section: Models and Methodsmentioning
confidence: 99%
“…Our recent pilot studies suggest Nrf2 oscillation responds to a single bolus exposure of 10-20 μM H 2 O 2 in cultured cells (Xue, M., Rabbani N. and Thornalley, P.J., unpublished observation); approximately 400 μM H 2 O 2 required to stabilize Nrf2 protein [27]. Given the high rate of metabolism of exogenous H 2 O 2 [28], the lower bolus exposure may induce responses similar to those of endogenous oxidative challenge to homoeostasis.…”
Section: Oscillation Frequency Responds To Physiological Levels Of Acmentioning
confidence: 98%