An assay for measuring specific adhesion of anEscherichia coli strain to tissue culture cells

Abstract

Escherichia coli SS142 has been found to adhere specifically to the human epithelioid tissue culture cell line Intestine 407, but not to other tissue culture cells. This paper describes an accurate, reproducible and objective method of assessing the rate of adhesion of radiolabelled bacteria to these cellular monolayers. Adhesion was found to be linear with time for 60 min and with bacterial concentrations up to 10(9) bacteria/ml. The binding appeared to be irreversible. Adhesion was not affected by changes in the composition of the medium, its pH or ionic strength, or by the assay temperature within physiological limits, but was diminished at very high ionic strength or low temperature. It increased with increasing cell density of the monolayers. Under appropriate conditions the assay could be used for comparative determinations of the rate of adhesion of different, or differently treated bacteria.

Haemagglutinins and Adhesion of Escherichia Coli To Hep2 Epithelial Cells

“…Thus, regardless of whether strains of E. coli produced type-1 fimbriae or one of three different antigenic types of MRE fimbriae, none adhered as efficiently to HEp2 cells in this model as the type-1 fimbriate strain S. typhimurium S6354. It is clear, therefore, from this and other studies (Vosbeck and Mett, 1983) that there is no correlation among urinary strains of E. coli between production of MR adhesin and ability to adhere to different tissue-culture lines, though occasional MREHA + strains of urinary origin apparently adhere well to some kinds of tissue-culture cells (Vosbeck and Huber, 1982).…”

A bath challenge model for furunculosis in rainbow trout, Salmo gairdneri Richardson, and Atlantic salmon, Salmo salar L.

“…Adhesion of Aeromonas salmonicida to Atlantic salmon cells was performed using a modification of the method of Vosbeck & Huber (1982).…”

Adhesiveness and invasiveness of staphylococcal species in a cell culture model

“…We are aware of the fact that different tissues have different affinities for microbial agents, depending on surface structures and receptor function. We do not know whether HEp-2 cells are representative of urinary tract epithelium, but it was demonstrated that in contrast to randomly selected E. coli strains from the fecal flora a uropathogenic strain of E. coli showed specific attachement to HEp-2 cells and other epithelioid cells in contrast to cells from other origins (30). Thus, our model seem to reflect a correlation between pathogenicity within the genus and invasion or adhesion.…”