An assay for inorganic mercury(II) based on its post‐catalytic enhancement effect on the potassium permanganate–luminol system

Abstract

A strong chemiluminescence (CL) response is observed when potassium permanganate solution is injected into basic luminol solution. When the CL reaction terminates, subsequent injection of Hg2+ solution into the reaction mixture results in a new CL reaction. Based on the post-catalytic enhancement effect of Hg2+ on the potassium permanganate-luminol system in basic media, a fast and simple CL-coupled flow injection analysis for the determination of Hg2+ was developed. In optimum conditions, CL intensity is proportional to Hg2+ concentration over the range 1.0 x 10(-8)-1.0 x 10(-5) g/mL, with a detection limit of 2.0 x 10(-9) g/mL. The relative standard deviation (RSD) is 3.6% for 1.0 x 10(-7) g/mL Hg2+ (n = 11). After pretreatment with sulphydryl cotton fibre, environmental water samples were analysed by the proposed method for total mercury determination with satisfactory results. The results were in good agreement with those given by hydride generation-cold vapour atomic absorption spectrometry (HG-CVAAS).

A High Sensitivity Micro Format Chemiluminescence Enzyme Inhibition Assay for Determination of Hg(II)

“…In an optical microplate assay, product formation can be measured directly without any prior analyte preconcentration step or a later purification step for product. It is reported that among the optical techniques, chemiluminescence can be used for heavy metal analysis as the instrumental setup is relatively simple, free from instrumental interferences and highly sensitive [ 26 – 28 ]. There is significant interest toward miniaturization of analytical systems, since it allows the handling of low volume samples, reduction in waste generation and reagent consumption with increased sample throughput [ 29 , 30 ].…”

Determination of protoberberine alkaloids in medicinal plants based on acidic potassium permanganate chemiluminescence system

“…[21] In addition, Hu et al reported a CL method for the determination of inorganic mercury (II) based on its post-catalytic enhancement eff ect on the potassium permanganate-luminol system. [22] Recently, there have been four reported procedures in the literatures for the determination of berberine in tablet or capsules using fl ow injection with CL detection. These procedures are based on the inhibition of berberine hydrochloride in the CL reaction of luminol-vanadomolybdophosphoric heteropolyacid-sodium hydroxide system, [23] the enhancement action of berberine hydrochloride on the weak CL of a cobalt (II)-hydrogen peroxide-hydrogencarbonate system [24] or cerium (IV)-Ru (bipy) 3 2+ system, [25] and the CL reaction of a potassium permanganate-berberine hydrochloride-polyphosphoric acid system.…”