An aseptically operatable static solid state bioreactor consisting of two units

Lignin-modifying enzymes have various promising applications such as biobleaching, biopulping, the functionalization of lignocellulosic materials, the modification of wood fibers, the remediation of contaminated soil and effluents, as well as improvement of the enzymatic hydrolysis of lignocellulosic substrates. In this study, the production of laccase and manganese peroxidase (MnP) in solid-state cultivation was examined. Oat husks were used as an inexpensive substrate for the white-rot fungus Cerrena unicolor PM170798 (FBCC 387). The addition of a fines fraction (consisting of oat flour and finely ground husks) enhanced MnP production fivefold and laccase production almost threefold. The enzyme production was studied first on a 100 g scale, and the cultivation experiments were then repeated at a larger laboratory-scale (4 kg) in a solid-state bioreactor. High enzyme activity levels were obtained (MnP: 340 nkat g(-1) DM, laccase: 470 nkat g(-1) DM). In addition, the correlation between the CO2 evolution rate and enzyme production was mathematically modeled from the bioreactor experimental data. The model parameters could be used to predict enzyme production.

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“…biopesticides. The reactor configurations vary from trays to mixed packed beds, and the volumes range from 50 to 500 L [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Production of manganese peroxidase and laccase in a solid-state bioreactor and modeling of enzyme production kinetics

Moilanen¹,

Winquist²,

Mattila³

et al. 2014

Bioprocess Biosyst Eng

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Mycelial inoculation of containerized Norway spruce seedlings with ectomycorrhizal fungi

Himanen

Nygren²,

Pennanen

2023

New Forests

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An inoculation technique to create ectomycorrhizal symbiosis in 1.5-year-old Norway spruce (Picea abies (L.) Karst.) container seedlings was tested. The mycelia of ectomycorrhizal fungi (EMF) – Tylospora asterophora, Piloderma olivaceum, and Cenococcum geophilum – each grown in a silica dioxide powder carrier, was mixed with a conventional low-humified Sphagnum peat at the time of sowing. Seedlings were grown in four growth media: (1) conventional peat; (2) conventional peat mixed with sterile carrier; (3) conventional peat mixed with carrier containing T. asterophora and C. geophilum, (4) conventional peat mixed with carrier containing P. olivaceum and C. geophilum. The seedling development and EMF colonization was followed during the nursery production. Further, seedlings grown in the four media were planted on a former nursery field, and their development was observed for three years. At the end of the nursery production phase, there were no differences in the seedling height or stem diameter between the growing media. The colonization degree by the introduced EMF was low. The height growth of the seedlings inoculated with T. asterophora + C. geophilum was 16% higher during the first growing season after out-planting compared to seedlings grown in the conventional peat medium, but the effect was transient. At the end of the out-planting experiment, the seedlings grown in conventional peat had the highest proportion of healthy and lowest proportion of dead seedlings. The results emphasize the importance of the growing media for seedling quality and out-planting success. The tested inoculation technique was ineffective in creating substantial levels of EMF colonization.

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Solid-State Fermentation: Applications and Future Perspectives for Biostimulant and Biopesticides Production

et al. 2023

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With the expansion of the green products market and the worldwide policies and strategies directed toward a green revolution and ecological transition, the demand for innovative approaches is always on the rise. Among the sustainable agricultural approaches, microbial-based products are emerging over time as effective and feasible alternatives to agrochemicals. However, the production, formulation, and commercialization of some products can be challenging. Among the main challenges are the industrial production processes that ensure the quality of the product and its cost on the market. In the context of a circular economy, solid-state fermentation (SSF) might represent a smart approach to obtaining valuable products from waste and by-products. SSF enables the growth of various microorganisms on solid surfaces in the absence or near absence of free-flowing water. It is a valuable and practical method and is used in the food, pharmaceutical, energy, and chemical industries. Nevertheless, the application of this technology in the production of formulations useful in agriculture is still limited. This review summarizes the literature dealing with SSF agricultural applications and the future perspective of its use in sustainable agriculture. The survey showed good potential for SSF to produce biostimulants and biopesticides useful in agriculture.

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An aseptically operatable static solid state bioreactor consisting of two units

Cited by 4 publications

References 7 publications

Production of manganese peroxidase and laccase in a solid-state bioreactor and modeling of enzyme production kinetics

Production of manganese peroxidase and laccase in a solid-state bioreactor and modeling of enzyme production kinetics

Mycelial inoculation of containerized Norway spruce seedlings with ectomycorrhizal fungi

Solid-State Fermentation: Applications and Future Perspectives for Biostimulant and Biopesticides Production

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