An arrayed library enriched in hncDNA corresponding to transcribed sequences of human chromosome 19: preparation and analysis

Borodin, A. M.; Kopatnzev, E.; Wagner, Lukas; Volik, Stanislav V.; Ermolaeva, Olga; Lebedev, Yuri B; Monastyrskaya, G.S.; Kunz, Jürgen; Grzeschik, Karl‐Heinz

doi:10.1016/1050-3862(95)00106-9

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…5). In these experiments, interAlu products from (a) SgrA I and Fse I cloneless fractions (b) YAC or (c) hncDNAs (generated as described in [27]) were arrayed onto nylon membranes and probed with labeled interAlu products generated from the cloneless library. Here, the interAlu products were used so that the experimental sample complexity was reduced and the hybridization signal stronger.…”

Section: Interalu Fingerprinting and Analysis Of Cloneless Fractionsmentioning

confidence: 99%

Cloneless genomic DNA analysis: an efficient and simple methods for de novo genomic sequencing projects and gap filling

Nguyen¹,

Bukanov²,

Oshimura

et al. 2005

Biomolecular Engineering

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Section: Interalu Fingerprinting and Analysis Of Cloneless Fractionsmentioning

confidence: 99%

Cloneless genomic DNA analysis: an efficient and simple methods for de novo genomic sequencing projects and gap filling

Nguyen¹,

Bukanov²,

Oshimura

et al. 2005

Biomolecular Engineering

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Construction of a Chromosome Specific Library of Human Mars and Mapping of Matrix Attachment Regions on Human Chromosome 19

Николаев

Tsevegiyn

Akopov

et al. 1996

Nucleic Acids Research

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Using a novel procedure a representative human chromosome 19-specific library was constructed of short sequences, which bind preferentially to the nuclear matrix (matrix attachment regions, or MARs). Judging by 20 clones sequenced so far, the library contains > 50% of human inserts, about 90% of which are matrix-binding by the in vitro test. Computer analysis of sequences of eight human MARs did not reveal any significant homologies with the EMBL Nucleotide Data Base entries as well as between MARs themselves. Eight MARs were assigned to individual positions on the chromosome 19 physical map. The library constructed can serve as a good source of MAR sequences for comparative analysis and classification and for further chromosome mapping of MARs as well.

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Mapping of transcribed sequences on human chromosome 19

et al. 1995

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30 EST/STS have been mapped on human chromosome 19 using a highly specific hncDNA library as a source of transcribed sequences. In addition more than 50 sites constituting 19 families of closely related sequences containing at least one transcribed member each were mapped across the chromosome. Chromosome-19 specific hncDNA clones were hybridized to chromosome 19 cosmids that were previously assembled into contigs covering about 80% of Chr19. The hybridization results were verified by PCR. Such an approach to EST mapping provides information on possible locations of genes as transcribed units of genome and on location of repeated elements used for the priming the hncDNA synthesis. Mapped hncDNA sequences may serve as good starting points for the systematic sequencing of transcribed genomic regions.

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An arrayed library enriched in hncDNA corresponding to transcribed sequences of human chromosome 19: preparation and analysis

Cited by 3 publications

References 31 publications

Cloneless genomic DNA analysis: an efficient and simple methods for de novo genomic sequencing projects and gap filling

Cloneless genomic DNA analysis: an efficient and simple methods for de novo genomic sequencing projects and gap filling

Construction of a Chromosome Specific Library of Human Mars and Mapping of Matrix Attachment Regions on Human Chromosome 19

Mapping of transcribed sequences on human chromosome 19

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