An array of possibilities for pectin

Sørensen, Iben; Pedersen, Henriette L.; Willats, William G.T.

doi:10.1016/j.carres.2008.12.008

Cited by 26 publications

(12 citation statements)

References 35 publications

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“…Recently, methods based on carbohydrate microarrays probed with monoclonal antibodies (mAbs) and carbohydrate-binding modules (CBMs) with specificities for cell wall polysaccharide epitopes have been developed. This technology can enable analysis of the occurrence of 100 epitopes in 2–3 days and can reveal much about cell wall composition despite some limitations largely derived from the current, although increasing, availability of characterized mAbs and CBMs (Moller et al, 2007; Sørensen et al, 2009; Pattathil et al, 2010). Application of FTIR and carbohydrate microarrays can be used to indicate cell walls that have a composition that may merit further and detailed analysis because they appear to be significantly different from characterized cell walls.…”

Section: Challenges In Surveying Cell Wall Diversitymentioning

confidence: 99%

Cell wall evolution and diversity

et al. 2012

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Plant cell walls display a considerable degree of diversity in their compositions and molecular architectures. In some cases the functional significance of a particular cell wall type appears to be easy to discern: secondary cells walls are often reinforced with lignin that provides durability; the thin cell walls of pollen tubes have particular compositions that enable their tip growth; lupin seed cell walls are characteristically thickened with galactan used as a storage polysaccharide. However, more frequently the evolutionary mechanisms and selection pressures that underpin cell wall diversity and evolution are unclear. For diverse green plants (chlorophytes and streptophytes) the rapidly increasing availability of transcriptome and genome data sets, the development of methods for cell wall analyses which require less material for analysis, and expansion of molecular probe sets, are providing new insights into the diversity and occurrence of cell wall polysaccharides and associated biosynthetic genes. Such research is important for refining our understanding of some of the fundamental processes that enabled plants to colonize land and to subsequently radiate so comprehensively. The study of cell wall structural diversity is also an important aspect of the industrial utilization of global polysaccharide bio-resources.

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Section: Challenges In Surveying Cell Wall Diversitymentioning

confidence: 99%

Cell wall evolution and diversity

et al. 2012

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“…Moreover these interactions are only possible while in the pH range of 2.0e3.8, reducing negative charges along the pectin chain, due to carboxylic acid groups' dissociation (Da Silva et al, 1995). This type of gelling is represented in a variety of commercial applications of pectin as for example some jams and jellies (Sørensen, Pedersen, & Willats, 2008).…”

Section: Introductionmentioning

confidence: 98%

The impact of rhamnogalacturonan-I side chain monosaccharides on the rheological properties of citrus pectin

Sousa

Nielsen²,

Armagan³

et al. 2015

Food Hydrocolloids

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“…CoMPP does not provide information about the absolute levels of polysaccharides, but compared to conventional techniques for cell wall analysis such as monosaccharide composition analysis, CoMPP has the advantage that it provides information about glycan epitopes that can be assigned with confidence to specific polysaccharides. The technique is well suited for tracking detailed changes in cell wall components in complex biological systems and particularly so when a paucity of prior information about cell wall composition complicates the interpretation of data from conventional biochemical techniques [35], [36], [38]. We therefore used CoMPP to map the distribution of polysaccharide epitopes within consecutive sections of the fungus garden of Acromyrmex echinatior leaf-cutting ants, as well as in the leaves provided as forage and the debris discarded by the ants ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

The Dynamics of Plant Cell-Wall Polysaccharide Decomposition in Leaf-Cutting Ant Fungus Gardens

et al. 2011

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The degradation of live plant biomass in fungus gardens of leaf-cutting ants is poorly characterised but fundamental for understanding the mutual advantages and efficiency of this obligate nutritional symbiosis. Controversies about the extent to which the garden-symbiont Leucocoprinus gongylophorus degrades cellulose have hampered our understanding of the selection forces that induced large scale herbivory and of the ensuing ecological footprint of these ants. Here we use a recently established technique, based on polysaccharide microarrays probed with antibodies and carbohydrate binding modules, to map the occurrence of cell wall polymers in consecutive sections of the fungus garden of the leaf-cutting ant Acromyrmex echinatior. We show that pectin, xyloglucan and some xylan epitopes are degraded, whereas more highly substituted xylan and cellulose epitopes remain as residuals in the waste material that the ants remove from their fungus garden. These results demonstrate that biomass entering leaf-cutting ant fungus gardens is only partially utilized and explain why disproportionally large amounts of plant material are needed to sustain colony growth. They also explain why substantial communities of microbial and invertebrate symbionts have evolved associations with the dump material from leaf-cutting ant nests, to exploit decomposition niches that the ant garden-fungus does not utilize. Our approach thus provides detailed insight into the nutritional benefits and shortcomings associated with fungus-farming in ants.

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An array of possibilities for pectin

Cited by 26 publications

References 35 publications

Cell wall evolution and diversity

Cell wall evolution and diversity

The impact of rhamnogalacturonan-I side chain monosaccharides on the rheological properties of citrus pectin

The Dynamics of Plant Cell-Wall Polysaccharide Decomposition in Leaf-Cutting Ant Fungus Gardens

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