An arginine decarboxylase gene PtADC from Poncirus trifoliata confers abiotic stress tolerance and promotes primary root growth in Arabidopsis

Wang, Jing; Sun, Peilong; Chen, Chunli; Wang, Yin; Fu, Xing-Zheng; Liu, Jihong

doi:10.1093/jxb/erq463

Cited by 158 publications

(123 citation statements)

References 55 publications

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“…At the same time, some previous reports have demonstrated antioxidant roles of PAs (Moschou et al 2008;Tavladoraki et al 2011). Recently, it has been demonstrated, for overexpressing of PtADC in an adc1-1 mutant that the highest PA content (in this case, Put) plays a positive role in alleviating ROS injury (Wang et al 2011). This agrees with our results because PIC AZ-AC accumulated less H 2 O 2 and high levels of free and conjugated Put in response to ET compared with ARB AZ-AC.…”

Section: Interaction Between H 2 O 2 and Abscissionmentioning

confidence: 90%

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Gil-Amado

Gómez-Jiménez

2011

Planta

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Exogenous ethylene and some inhibitors of polyamine biosynthesis can induce mature-fruit abscission in olive, which could be associated with decreased nitric oxide production as a signaling molecule. Whether H₂O₂ also plays a signaling role in mature-fruit abscission is unknown. The possible involvement of H₂O₂ and polyamine in ethylene-induced mature-fruit abscission was examined in the abscission zone and adjacent cells of two olive cultivars. Endogenous H₂O₂ showed an increase in the abscission zone during mature-fruit abscission, suggesting that accumulated H₂O₂ may participate in abscission signaling. On the other hand, we followed the expression of two genes involved in the polyamine biosynthesis pathway during mature-fruit abscission and in response to ethylene or inhibitors of ethylene and polyamine. OeSAMDC1 and OeSPDS1 were expressed differentially within and between the abscission zones of the two cultivars. OeSAMDC1 showed slightly lower expression in association with mature-fruit abscission. Furthermore, our data show that exogenous ethylene or inhibitors of polyamine encourage the free putrescine pool and decrease the soluble-conjugated spermidine, spermine, homospermidine, and cadaverine in the olive abscission zone, while ethylene inhibition by CoCl₂ increases these soluble conjugates, but does not affect free putrescine. Although the impact of these treatments on polyamine metabolism depends on the cultivar, the results confirm that the mature-fruit abscission may be accompanied by an inhibition of S-adenosyl methionine decarboxylase activity, and the promotion of putrescine synthesis in olive abscission zone, suggesting that endogenous putrescine may play a complementary role to ethylene in the normal course of mature-fruit abscission.

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Section: Interaction Between H 2 O 2 and Abscissionmentioning

confidence: 90%

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Gil-Amado

Gómez-Jiménez

2011

Planta

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“…Determination of electrolyte leakage (EL) and free proline content EL was measured according to the method of Wang et al (2011). The leaves were cut into strips and incubated in 10 ml of distilled water.…”

Section: Observation Of Am Developmentmentioning

confidence: 99%

Colonization with arbuscular mycorrhizal fungus affects growth, drought tolerance and expression of stress-responsive genes in Poncirus trifoliata

Fan

Liu

2011

Acta Physiol Plant

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The objective of this study was to investigate the effects of arbuscular mycorrhizal fungus (AMF) inoculation on growth and drought tolerance of Poncirus trifoliata seedlings. The seedlings were inoculated with or without Glomus mosseae before exposure to a short-term (3 days) water depletion, and relevant physiological and biochemical parameters (plant height, chlorophyll content, relative water content, activity of antioxidant enzymes) and expression patterns of several stress-responsive genes were examined. Inoculation with G. mosseae led to growth promotion of the seedlings, as revealed by larger plant height and higher relative water and chlorophyll contents. When subjected to drought treatment, the AMF-inoculated (AM) plants showed better tolerance than the nonmycorrhizal (NAM) plants. Under drought, the AM plants exhibited higher level of proline and activity of two antioxidant enzymes, superoxide dismutase (SOD) and peroxidase (POD). In addition, mRNA abundance of four genes involved in reactive oxygen species homeostasis and oxidative stress battling was higher in the AM plants when compared with the NAM plants. These results indicate that AMF inoculation stimulated growth and enhanced drought tolerance of the seedlings, which may be due to activation of an arsenal of physiological, biochemical and molecular alterations.

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“…Arginine decarboxylase, hsp70, hsp 81-2, superoxide dismutase, glutathione peroxidise, cinnamic acid 4-hydroxylase and ACC oxidase are some of the examples of stress related genes. Arginine decarboxylase (ADC) is not only capable of enhancing ability of scavenging reactive oxygen species (ROS) but also has been shown to be involved in root growth as transgenic with ADC had longer roots compared to wild type (Wang et al 2013). Similarly, heat shock proteins are well known stress proteins but also shown to perform root specific functions.…”

Section: Discussionmentioning

confidence: 99%

Identification of key genes involved in root development of tomato using expressed sequence tag analysis

Kalidhasan

Joshi

Bhatt

et al. 2015

Physiol Mol Biol Plants

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Root system of plants are actually fascinating structures, not only critical for plant development, but also important for storage and conduction. Due to its agronomic importance, identification of genes involved in root development has been a subject of intense study. Tomato is the one of the most consumed vegetables in the world. Tomato has been used as model system for dicot plants because of its small genome, well-established transformation techniques and well-constructed physical map. The present study is targeted to identify of root specific genes expressed temporally and also gene(s) involved in lateral root and profuse root development. A total of 890 ESTs were identified from five EST libraries constructed using SSH approach which included temporal gene regulation (early and late) and genes involved in morphogenetic traits (lateral and profuse rooting). One hundred sixty-one unique ESTs identified from various libraries were categorized based on their putative functions and deposited in NCBI-dbEST database. In addition, 36 ESTs were selected for validation of their expression by RT-PCR. The present findings will help in shedding light to the unexplored developmental process of root growth in tomato and plant in general.

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An arginine decarboxylase gene PtADC from Poncirus trifoliata confers abiotic stress tolerance and promotes primary root growth in Arabidopsis

Cited by 158 publications

References 55 publications

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Colonization with arbuscular mycorrhizal fungus affects growth, drought tolerance and expression of stress-responsive genes in Poncirus trifoliata

Identification of key genes involved in root development of tomato using expressed sequence tag analysis

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