An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics

Rosso, Mario G.; Li, Yong; Strizhov, N.; Reiss, Bernd; Dekker, Koen; Weißhaar, Bernd

doi:10.1023/b:plan.0000009297.37235.4a

Cited by 681 publications

(702 citation statements)

References 36 publications

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“…This line contains an insertion 5 bp upstream from the start codon of the PMM gene, as verified via the standard GABI-Kat quality control on DNA extracted from T 2 seedlings (29). Unfortunately, none of the T 2 plants grown from the received seeds gave T 3 seeds that were 100% resistant to sulfadiazine.…”

Section: Identification Of a Ts Cell Deathmentioning

confidence: 99%

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A Temperature-sensitive Mutation in the Arabidopsis thaliana Phosphomannomutase Gene Disrupts Protein Glycosylation and Triggers Cell Death

Hoeberichts

Vaeck

Kiddle

et al. 2008

Journal of Biological Chemistry

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Eukaryotic phosphomannomutases (PMMs) catalyze the interconversion of mannose 6-phosphate to mannose 1-phosphate and are essential to the biosynthesis of GDP-mannose. As such, plant PMMs are involved in ascorbic acid (AsA) biosynthesis and N-glycosylation. We report on the conditional phenotype of the temperature-sensitive Arabidopsis thaliana pmm-12 mutant. Mutant seedlings were phenotypically similar to wild type seedlings when grown at 16 -18°C but died within several days after transfer to 28°C. This phenotype was observed throughout both vegetative and reproductive development. Protein extracts derived from pmm-12 plants had lower PMM protein and enzyme activity levels. In vitro biochemical analysis of recombinant proteins showed that the mutant PMM protein was compromised in its catalytic efficiency (K cat /K m ). Despite significantly decreased AsA levels in pmm-12 plants, AsA deficiency could not account for the observed phenotype. Since, at restrictive temperature, total glycoprotein patterns were altered and glycosylation of protein-disulfide isomerase was perturbed, we propose that a deficiency in protein glycosylation is responsible for the observed cell death phenotype.

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Section: Identification Of a Ts Cell Deathmentioning

confidence: 99%

“…We retrieved the T-DNA insertion line 045D04 from the GABI-Kat collection (29). This line contains an insertion 5 bp upstream from the start codon of the PMM gene, as verified via the standard GABI-Kat quality control on DNA extracted from T 2 seedlings (29).…”

Section: Identification Of a Ts Cell Deathmentioning

confidence: 99%

A Temperature-sensitive Mutation in the Arabidopsis thaliana Phosphomannomutase Gene Disrupts Protein Glycosylation and Triggers Cell Death

Hoeberichts

Vaeck

Kiddle

et al. 2008

Journal of Biological Chemistry

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“…The T-DNA insertion lines were obtained from the following sources: oep80-1 was from the Csaba Koncz laboratory (pool 894, line 89350; Ríos et al, 2002); and oep80-2 and oep80-3 were from Genomanalyse im Biologischen System Pflanze-Kölner Arabidopsis T-DNA (GABI-Kat; lines 429H12 and 430F02, respectively; Rosso et al, 2003).…”

Section: Identification Of the Oep80 Mutantsmentioning

confidence: 99%

The Omp85-Related Chloroplast Outer Envelope Protein OEP80 Is Essential for Viability in Arabidopsis

et al. 2008

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b-Barrel proteins of the Omp85 (Outer membrane protein, 85 kD) superfamily exist in the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Prominent Omp85 proteins in bacteria and mitochondria mediate biogenesis of other b-barrel proteins and are indispensable for viability. In Arabidopsis (Arabidopsis thaliana) chloroplasts, there are two distinct types of Omp85-related protein: Toc75 (Translocon at the outer envelope membrane of chloroplasts, 75 kD) and OEP80 (Outer Envelope Protein, 80 kD). Toc75 functions as a preprotein translocation channel during chloroplast import, but the role of OEP80 remains elusive. We characterized three T-DNA mutants of the Arabidopsis OEP80 (AtOEP80) gene. Selectable markers associated with the oep80-1 and oep80-2 insertions segregated abnormally, suggesting embryo lethality of the homozygous genotypes. Indeed, no homozygotes were identified among .100 individuals, and heterozygotes of both mutants produced approximately 25% aborted seeds upon self-pollination. Embryo arrest occurred at a relatively late stage (globular embryo proper) as revealed by analysis using Nomarski optics microscopy. This is substantially later than arrest caused by loss of the principal Toc75 isoform, atToc75-III (two-cell stage), suggesting a more specialized role for AtOEP80. Surprisingly, the oep80-3 T-DNA (located in exon 1 between the first and second ATG codons of the open reading frame) did not cause any detectable developmental defects or affect the size of the AtOEP80 protein in chloroplasts. This indicates that the N-terminal region of AtOEP80 is not essential for the targeting, biogenesis, or functionality of the protein, in contrast with atToc75-III, which requires a bipartite targeting sequence.

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“…For some of the MIOX genes, T-DNA insertion lines (DMIOX1, DMIOX2 and DMIOX5) were isolated from the SALK collection (Alonso et al 2003) or the GABI-KAT collection (Rosso et al 2003, http://www.gabikat.de). Homozygous knock-outs in single isoforms were analyzed for phenotypic changes compared to the wild type.…”

Section: Mutant Lines For Mioxmentioning

confidence: 99%

The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides

Kanter

Usadel

Guérineau

et al. 2005

Planta

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141

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The nucleotide sugar UDP-glucuronic acid (UDP-GlcA) is the principal precursor for galacturonic acid, xylose, apiose and arabinose residues of the plant cell-wall polymers. UDP-GlcA can be synthesized by two different functional pathways in Arabidopsis involving either UDP-glucose dehydrogenase or inositol oxygenase as the initial enzyme reaction to channel carbohydrates into a pool of UDP sugars used for cellwall biosynthesis. The genes for the enzyme myo-inositol oxygenase (MIOX) were analyzed in Arabidopsis. They represent a small gene family containing four members. The transcription of all those members indicates a transient and organ-specific gene expression pattern in growing plant tissues as analyzed by RT-PCR and in promoter::GUS reporter gene lines. Two isoforms (MIOX1, MIOX2) are expressed in almost all tissues of the plant, whereas the expression of MIOX4 and MIOX5 is largely restricted to flowers, particularly maturing pollen. T-DNA insertion lines in MIOX genes were isolated; however, single knock-outs show growth phenotypes similar to the wild type. The monosaccharide composition of the cell wall in these mutants is not significantly changed compared to wild type plants. However, the incorporation of 3 H-inositol into wall polymers of seedlings is greatly impaired in the mutant lines DMIOX1 and DMIOX2, which are the only isoforms that are expressed in seedlings.

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An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics

Cited by 681 publications

References 36 publications

A Temperature-sensitive Mutation in the Arabidopsis thaliana Phosphomannomutase Gene Disrupts Protein Glycosylation and Triggers Cell Death

A Temperature-sensitive Mutation in the Arabidopsis thaliana Phosphomannomutase Gene Disrupts Protein Glycosylation and Triggers Cell Death

The Omp85-Related Chloroplast Outer Envelope Protein OEP80 Is Essential for Viability in Arabidopsis

The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides

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