An Arabidopsis PWI and RRM motif-containing protein is critical for pre-mRNA splicing and ABA responses

Zhan, Xiangqiang; Qian, Bilian; Cao, Fengming; Wu, Wenwu; Yang, Lan; Guan, Qingmei; Gu, Xianbin; Wang, Pengcheng; Okusolubo, Temiloluwa A.; Dunn, Stephanie L.; Zhu, Jian‐Kang; Zhu, Jianhua

doi:10.1038/ncomms9139

Cited by 94 publications

(107 citation statements)

References 68 publications

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“…Abscisic acid (ABA) is a phytohormone important for stress responses including salt stress response (Park et al 2009). In the candidate genes, the orthologs of GRMZM2G166218 and GRMZM2G127035 have been reported to be involved in regulation of ABA signaling (Liu et al 2010;Zhan et al 2015). The ortholog of GRMZM2G001289 regulated stomatal development (Peterson et al 2013).…”

Section: Characterization Of the Candidate Genesmentioning

confidence: 99%

Genome‐wide association study dissects the genetic bases of salt tolerance in maize seedlings

Luo

Wang

Gao

et al. 2019

JIPB

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Excess salinity is a natural stress that causes crop yield losses worldwide. The genetic bases of maize salt tolerance remain largely unknown. Here we investigated the survival rates of 445 maize natural accessions after salt treatments. A skewed distribution of the salt‐tolerant phenotypes was observed in this population. Genome‐wide association studies (GWAS) revealed 57 loci significantly associated with salt tolerance. Forty‐nine candidate genes were detected from these loci. About 10% of these genes were co‐localized with loci from QTL mapping. Forty four percent of the candidate genes were involved in stress responses, ABA signaling, stomata division, DNA binding/transcription regulation and auxin signaling, suggesting that they are key genetic mechanisms of maize salt tolerance. Transgenic studies showed that two genes, the salt‐tolerance‐associated‐gene 4 (SAG4, GRMZM2G077295) and SAG6 (GRMZM2G106056), which encode a protein transport protein and the double‐strand break repair protein MRE11, respectively, had positive roles in plant salt tolerance, and their salt‐tolerant haplotypes were revealed. The genes we identified in this study provide a list of candidate targets for further study of maize salt tolerance, and of genetic markers and materials that may be used for breeding salt‐tolerance in maize.

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Section: Characterization Of the Candidate Genesmentioning

confidence: 99%

Genome‐wide association study dissects the genetic bases of salt tolerance in maize seedlings

Luo

Wang

Gao

et al. 2019

JIPB

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“…Eight genes encoding splicing factors (Koncz et al, 2012) showed abnormal splicing in atu2af65b-1 (Tables S4, S6). Several flowering-related genes (FLC, MAF1, MAF2, MAF3 and SNZ) and ABA signalingrelated genes (DWA3, HAB1, RAF10, GRP23, and ABI5) (Park et al, 2008;Lee et al, 2011Lee et al, , 2015Wang et al, 2015;Zhan et al, 2015;Gras et al, 2018) are in the list of abnormal splicing in atu2af65b-1 ( Fig. 8d; Tables S4, S6).…”

Section: Atu2af65b Is Involved In Splicing Of a Subset Of Genesmentioning

confidence: 99%

AtU2AF65b functions in abscisic acid mediated flowering via regulating the precursor messenger RNA splicing of ABI5 and FLC in Arabidopsis

Xiong

Ren

et al. 2019

New Phytologist

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Summary In mammalians and yeast, the splicing factor U2AF65/Mud2p functions in precursor messenger RNA (pre‐mRNA) processing. Arabidopsis AtU2AF65b encodes a putative U2AF65 but its specific functions in plants are unknown. This paper examines the function of AtU2AF65b as a negative regulator of flowering time in Arabidopsis. We investigated the expression and function of AtU2AF65b in abscisic acid (ABA)‐regulated flowering as well as the transcript abundance and pre‐mRNA splicing of flowering‐related genes in the knock‐out mutants of AtU2AF65b. The atu2af65b mutants show early‐flowering phenotype under both long‐day and short‐day conditions. The transcript accumulation of the flowering repressor gene FLOWERING LOCUS C (FLC) is reduced in the shoot apex of atu2af65b, due to both increased intron retention and reduced transcription activation. Reduced transcription of FLC results, at least partially, from the abnormal splicing and reduced transcript abundance of ABSCISIC ACID‐INSENSITIVE 5 (ABI5), which encodes an activator of FLC in ABA‐regulated flowering signaling. Additionally, the expression of AtU2AF65b is promoted by ABA. Transition to flowering and splicing of FLC and ABI5 in the atu2af65b mutants are compromised during ABA‐induced flowering. ABA‐responsive AtU2AF65b functions in the pre‐mRNA splicing of FLC and ABI5 in shoot apex, whereby AtU2AF65b is involved in ABA‐mediated flowering transition in Arabidopsis.

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“…Whereas transcriptome profiling has highlighted shift in the expression and dominance of certain isoforms of splicing factors and splice variants accompanying stress response (Liu et al, ; Zhang et al, ), the mode of implementation of this regulation is far from clear. Increasing evidence suggest that ABA‐mediated response is mediated by pre‐mRNA splicing (Haak et al, ; Zhan et al, ). The isoforms and altered proteins arising from alternative splicing are indeed known to potentially assist in stress tolerance (Kamies, Farrant, Tadele, Cannarozzi, & Rafudeen, ).…”

Section: Introductionmentioning

confidence: 99%

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

Barua

Lande

Subba

et al. 2018

Plant Cell & Environment

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Nonavailability of water or dehydration remains recurring climatic disorder affecting yield of major food crops, legumes in particular. Nuclear proteins (NPs) and phosphoproteins (NPPs) execute crucial cellular functions that form the regulatory hub for coordinated stress response. Phosphoproteins hold enormous influence over cellular signalling. Four-week-old seedlings of a grain legume, chickpea, were subjected to gradual dehydration, and NPs were extracted from unstressed control and from 72- and 144-hr stressed tissues. We identified 4,832 NPs and 478 phosphosites, corresponding to 299 unique NPPs involved in multivariate cellular processes including protein modification and gene expression regulation, among others. The identified proteins included several novel kinases, phosphatases, and transcription factors, besides 660 uncharacterized proteins. Spliceosome complex and splicing related proteins were dominant among differentially regulated NPPs, indicating their dehydration modulated regulation. Phospho-motif analysis revealed stress-induced enrichment of proline-directed serine phosphorylation. Association mapping of NPPs revealed predominance of differential phosphorylation of spliceosome and splicing associated proteins. Also, regulatory proteins of key processes viz., protein degradation, regulation of flowering time, and circadian clock were observed to undergo dehydration-induced dephosphorylation. The characterization of novel regulatory proteins would provide new insights into stress adaptation and enable directed genetic manipulations for developing climate-resilient crops.

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An Arabidopsis PWI and RRM motif-containing protein is critical for pre-mRNA splicing and ABA responses

Cited by 94 publications

References 68 publications

Genome‐wide association study dissects the genetic bases of salt tolerance in maize seedlings

Genome‐wide association study dissects the genetic bases of salt tolerance in maize seedlings

AtU2AF65b functions in abscisic acid mediated flowering via regulating the precursor messenger RNA splicing of ABI5 and FLC in Arabidopsis

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

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