An Arabidopsis homologue of the Drosophila meiotic gene Pelota

Caryl, A. P.; LaCroix, Ian S; Jones, Gareth R.; Franklin, F. Christopher H.

doi:10.1007/s004970050200

Cited by 6 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of these six genes that was very significantly down-regulated at both time points was a Pelota gene (Additional file 3 : Table S7), which was originally identified in Drosophila melanogaster and is known to function in meiosis [ 62 ]. In mouse, it has been reported that disruption of the Pelota gene causes early embryonic lethality and defects in cell cycle progression [ 63 ], and in plants, a single homologue has been identified in Arabidopsis [ 64 ]. Due to its known role in meiosis, it is highly possible that reduction of Pelota expression in grape could be related to GA 3 -induced seedlessness (Figure 2 J).…”

Section: Discussionmentioning

confidence: 99%

Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca × V. vinifera) cv. Kyoho flowers

et al. 2015

View full text Add to dashboard Cite

BackgroundGibberellins are well known for their growth control function in flower, fruit and seed development, and as such, exogenous gibberellic acid (GA) application plays an important role in viticulture. Unfortunately, the mechanism by which GA3 acts in the regulation of these complicated developmental processes in grape remains unclear.ResultsIn the present study, we demonstrated that application of GA3 to ‘Kyoho’ grapevine inflorescences at pre-bloom promoted flower opening, and induced fruit coloring as well as seed abortion. In an attempt to obtain a deeper understanding of the molecular mechanisms driving these responses to GA3 treatment, we performed large-scale transcriptome sequencing of grape flowers following GA3 treatment using Illumina sequencing technology. Global expression profiles of GA3-treated and untreated grape flowers were compared and a large number of GA3-responsive genes were identified. Gene ontology (GO) term classification and biochemical pathway analyses indicated that GA3 treatment caused changes in the levels of transcripts involved in cellular processes, reproduction, hormone and secondary metabolism, as well as the scavenging and detoxification of reactive oxygen species (ROS). These findings suggest that GA3-induced morphological alterations may be related to the control of hormone biosynthesis and signaling, regulation of transcription factors, alteration of secondary metabolites, and the stability of redox homeostasis.ConclusionsTaken together, this comprehensive inflorescence transcriptome data set provides novel insight into the response of grape flowers to GA3 treatment, and also provides possible candidate genes or markers that could be used to guide future efforts in this field.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1324-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca × V. vinifera) cv. Kyoho flowers

et al. 2015

View full text Add to dashboard Cite

show abstract

“…No hybridization signals were detected even when 50 µg of total RNA was loaded (data not shown), suggesting that OsDMC1 is expressed at a low level. This difficulty has been encounted when analyzing expression of plant meiotic genes AtDMC1 (Klimyuk and Jones 1997), SYN1 (Bai et al 1999) and AtPelota (Caryl et al 2000) with northern hybridization. We therefore used RT-PCR to analyze expression of this gene.…”

Section: Expression Of Osdmc1mentioning

confidence: 99%

Isolation and characterization of OsDMC1 , the rice homologue of the yeast DMC1 gene essential for meiosis

Ding¹,

Wang²,

Chong³

et al. 2001

Sexual Plant Reproduction

View full text Add to dashboard Cite

Yeast DMC1 is a meiosis-specific gene required for homologous chromosome pairing in meiosis. Using degenerate primers designed according to amino acid motifs conserved in yeast Dmc1 and Arabidopsis AtDmc1, we obtained full-length cDNA of a rice homologue of the DMC1 gene (OsDMC1) by RT-PCR and rapid amplification of cDNA ends (RACEs). OsDmc1 exhibited 53% amino acid sequence identity to yeast Dmc1 and 81% to AtDmc1. OsDMC1 was expressed at high-levels in reproductive organs, low-levels in roots, and undetectable levels in leaves and seedlings. Southern blot analyses revealed that OsDMC1 is one of two DMC1 homologues present in rice.

show abstract

“…Pelota (PELO) is an evolutionarily conserved protein and has been identified in a number of species, including Drosophila (Eberhart and Wasserman, 1995), archaebacteria (Ragan et al, 1996), yeast (Davis and Engebrecht, 1998), Arabidopsis thaliana (Caryl et al, 2000), human (Shamsadin et al, 2000), mouse (Shamsadin et al, 2002) and tomato (Lapidot et al, 2015). The PELO proteins contain 347-395 amino acid residues and RNA-binding domains similar to that found in the family members of the eukaryotic release factor 1 (eRF1) family members, which play roles in termination of protein synthesis (Davis and Engebrecht, 1998).…”

Section: Introductionmentioning

confidence: 99%