An aqueous extract of the Anogeissus leiocarpus bark (AEAL) induces the endothelium-dependent relaxation of porcine coronary artery rings involving predominantly nitric oxide

Abstract

These findings indicate that AEAL is a potent inducer of endothelium-dependent NO-mediated relaxations in porcine coronary arteries through the redox-sensitive Src/PI3-kinase/Akt pathway-dependent activation of eNOS.

Qualitative Chemical Characterization and Multidirectional Biological Investigation of Leaves and Bark Extracts of Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae)

“…8-iso-PGF 2α , deriving from ROS/RNS peroxidation of membrane arachidonic acid, represents a stable marker of lipid peroxidation and tissue damage, in vivo [51], whereas the blunting effects on 8-iso-PGF 2α production that are induced by herbal extracts were related to protective effects [48,52]. Consistently with the reported antioxidant effects and the findings by Belemnaba et al [46], the tested extracts blunted LPS-induced 8-iso-PGF 2α production, with the only exception being represented by stem bark MeOH extract. On the other hand, stem bark MeOH extract showed the ability to reduce the level of malondialdehyde (MDA), which is another key marker of lipid peroxidation, in vivo [53].…”

“…Particularly, we selected the concentration 0.1 mg/mL that was at least two-fold lower than LC 50 and in agreement with previous investigations that demonstrated the antioxidant effects on isolated porcine tissue [46]. While considering these findings, we assayed extract effects on rat colon stimulated with LPS, ex vivo, in order reproduce the burden of oxidative stress and inflammation that characterize ulcerative colitis [20,47,48].…”

Preclinical Evaluation of the Antihypertensive Effect of an Aqueous Extract of Anogeissus leiocarpa (DC) Guill et Perr. Bark of Trunk in L-NAME-Induced Hypertensive Rat

“…It is for this interest that studies have already demonstrated the vasodilation effect of AEAL from Anogeissus leiocarpa. 18 AEAL were also known to be a potent inhibitor of purified cyclic nucleotide phosphodiesterase. 35 A recent study has also shown that a dichloromethane fraction from AEAL has an endothelium-independent vasodilator effect involving inhibition of PDE1, 2 and 5 and involve a reduction in intracellular calcium.…”

“… 25 , 40 , 41 Indeed, AEAL has been known to have vasodilation properties via Src/PI3-Akt kinase relaxation pathway responsible for the activation of eNOS followed by the release of NO. 18 Moreover, AEAL was known to inhibit in vitro, the purified cyclic nucleotides PDEs found in vascular smooth muscle cell (VSMC), whose might induce vessels relaxation. 35 Previous studies raised the presence of tannins, sterols and triterpenoids in AEAL, which were known to have vasodilator effect.…”

“…[14][15][16][17] Previous studies have shown that extracts from Anogeissus leiocarpa possess a potent antioxidant activity, an inhibitory effect of purified phosphodiesterases and an in vitro vasodilation effect. 18,19 Specifically, the AEAL is known to have an antihypertensive potential impact in the anesthetized model. 20 However, the long-term effects of Anogeissus leiocarpa on high blood pressure and arteriolar function from treated animals have not yet been reported.…”