An aptasensor for the detection of Mycobacterium tuberculosis secreted immunogenic protein MPT64 in clinical samples towards tuberculosis detection

Sypabekova, Marzhan; Dukenbayev, Kanat; Tsepke, Anna; Akisheva, Akmaral; Oralbayev, Nurlan; Kanayeva, Damira

doi:10.1038/s41598-019-52685-6

Cited by 23 publications

(18 citation statements)

References 32 publications

(28 reference statements)

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“…Recently, limited studies have been published on the detection of Mycobacteri a using EIS‐based techniques. For examples, Sypabekova et al reported EIS‐based detection of M. tuberculosis secreted protein MPT64 using an interdigitated electrode [36]. The authors applied a special receptor, a single stranded DNA aptamer, which specifically recognizes MPT64 protein.…”

Section: Resultsmentioning

confidence: 99%

Label‐free molecular detection of antibiotic susceptibility for Mycobacterium smegmatis using a low cost electrode format

Güzel

Ghorbanpoor

Dizaji

et al. 2020

Biotechnology and Applied Biochemistry

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Today, the emergence of antibiotic resistance in pathogenic bacteria is considered an important problem for society. Excessive consumption of antibiotics, long‐term treatments, and inappropriate prescriptions continually increase the severity of the problem. Improving antibiotic stewardship requires improved diagnostic testing, and, therefore, in vitro antibiotic susceptibility testing is becoming increasingly important. This research details the development of an antibiotic susceptibility test for Mycobacterium smegmatis using streptomycin as antibiotics. This strain was selected because it is a member of the slow growing Mycobacterium genus and serves as a useful surrogate organism for M. tuberculosis. A commercially available and low‐cost screen‐printed gold electrode in combination with a specifically developed nucleic acid probe sequence for the 16SrRNA region of the mycobacterial genome was employed to monitor M. smegmatis nucleic acid sequences using the techniques of square‐wave voltammetry and electrochemical impedance spectroscopy. The results show that it was possible to detect M. smegmatis sequences and distinguish antibiotic‐treated cells from untreated cells with a label‐free molecular detection. As a result, the in vitro antibiotic susceptibility test revealed that M. smegmatis showed sensitivity to streptomycin after a 24‐H incubation, with the developed protocol representing a potential approach to determining antibiotic susceptibility more quickly and economically than current methods.

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Section: Resultsmentioning

confidence: 99%

Label‐free molecular detection of antibiotic susceptibility for Mycobacterium smegmatis using a low cost electrode format

Güzel

Ghorbanpoor

Dizaji

et al. 2020

Biotechnology and Applied Biochemistry

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“…Most of the recent approaches use an aptamer element to engineer ligand-responsiveness and to regulate gene expression [23][24][25][26]44 . Aptamers are widely used, due to their high binding affinity against their respective target and their specificity [70][71][72][73][74][75][76][77][78][79][80][81][82] . In principal, the process of SELEX (Systematic Evolution of Ligands by Exponential enrichment) allows aptamer selection against any small molecule of interest 83,84 .…”

Section: Discussionmentioning

confidence: 99%

Translational control of enzyme scavenger expression with toxin-induced micro RNA switches

Pollak

Cooper-White

Macdonald

2021

Sci Rep

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Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic network to implement detoxification functionality. We employed a modular design strategy by engineering toxin-induced control of an enzyme scavenger. Our miRNA switch results show moderate synthetic expression control over a biologically active detoxification enzyme molecule, using an established design protocol. However, following a new design approach, we demonstrated an evolutionarily designed miRNA switch to more effectively activate enzyme activity than synthetically designed versions, allowing markedly improved extrinsic user-defined control with a toxin as inducer. Our straightforward new design approach is simple to implement and uses easily accessible web-based databases and prediction tools. The ability to exert control of toxicity demonstrates potential for modular detoxification systems that provide a pathway to new therapeutic and biocomputing applications.

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“…Sypabekova et al [18] presented results on the development of the aptasensor for the detection of MPT64, a Mycobacterium tuberculosis secreted protein, for tuberculosis (TB) diagnosis. This aptasensor used electrochemical impedance spectroscopy as determination method.…”

Section: Biomarker Detectionmentioning

confidence: 99%

Aptamer-Based Medical Devices

Liu

Wang

Zhang

2021

Aptamers for Medical Applications

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Medical devices have undergone an evolution over thousands of years with the intention to improve the health of people. Aptamers are single-stranded nucleotides that can specifically bind to their targets. They are promising alternatives of antibodies in the field of medical devices owing to the property of specific binding to their targets, as well as the advantages such as easy synthesis and modification, chemical stability, low immunogenicity, and competitive cost, etc. Nevertheless, to our knowledge, there are no aptamer-based medical devices officially approved in the market. Therefore, in this chapter, we first introduced the definition, history, and market of medical devices. Then, some potentially prototypical aptamer-based medical devices related clinical trials, patents, and papers were introduced from two aspects according to their application in "diagnosis" and "therapies," respectively. Finally, we proposed our perspectives and insights for the development of aptamerbased medical devices.

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An aptasensor for the detection of Mycobacterium tuberculosis secreted immunogenic protein MPT64 in clinical samples towards tuberculosis detection

Cited by 23 publications

References 32 publications

Label‐free molecular detection of antibiotic susceptibility for Mycobacterium smegmatis using a low cost electrode format

Label‐free molecular detection of antibiotic susceptibility for Mycobacterium smegmatis using a low cost electrode format

Translational control of enzyme scavenger expression with toxin-induced micro RNA switches

Aptamer-Based Medical Devices

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