An Aptamer Cross‐Linked Hydrogel as a Colorimetric Platform for Visual Detection

Zhu, Zhi; Wu, Cuichen; Liu, Haipeng; Zou, Yu; Zhang, Xiaoling; Kang, Houjun; Yang, Chaoyong; Tan, Weihong

doi:10.1002/anie.200905570

Cited by 333 publications

(245 citation statements)

References 63 publications

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“…11,12 Hydrogels are particularly attractive for making optical sensors. [13][14][15][16][17][18][19] Biomolecular immobilization occurs not only on gel surface but also throughout the whole gel matrix, allowing for high loading capacity. Due to its porous nature, all the immobilized probes are accessible to generate a strong signal.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine

Helwa

Dave

Froidevaux

et al. 2012

ACS Appl. Mater. Interfaces

116

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These authors contributed equally to the work. AbstractWith a low optical background, high loading capacity, and good biocompatibility, hydrogels are ideal materials for immobilization of biopolymers to develop optical biosensors. We recently immobilized mercury and lead binding DNAs within a monolithic gel and demonstrated ultra-sensitive visual detection of these heavy metals. The high sensitivity was attributed to the enrichment of the analytes into the gels.The signaling kinetics was slow, however, taking about one hour to obtain a stable optical signal due to a long diffusion distance. In this work we aim to understand the analyte enrichment process and improve the signaling kinetics by preparing hydrogel microparticles. DNA-functionalized gel beads were synthesized using an emulsion polymerization technique and most of the beads were between 10 and 50 μm. Acrydite-modified DNA was incorporated by co-polymerization. Visual detection of 10 nM Hg 2+ was still achieved and a stable signal was obtained in just two minutes. The gel beads could be spotted to form a microarray and dried for storage. A new visual sensor for adenosine was designed and immobilized within the gel beads. The adenosine aptamer binds its target about 1000-fold less tightly compared to the mercury binding DNA, allowing a comparison to be made on analyte enrichment by aptamer-functionalized hydrogels.

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Section: Introductionmentioning

confidence: 99%

Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine

Helwa

Dave

Froidevaux

et al. 2012

ACS Appl. Mater. Interfaces

116

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“…Other signaling mechanisms such as electrochemical detection have also been demonstrated while few examples of optical sensors are known (25,30,(45)(46)(47)(48)(49). In particular, very few colorimetric sensors have been reported (25,45,50,51). We are interested in making hydrogel-based colorimetric sensors since hydrogels are transparent and can therefore be observed with a low optical background, allowing low concentration of labels to be visually detected.…”

Section: Introductionmentioning

confidence: 99%

DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection

Baeissa

Dave

Smith

et al. 2010

ACS Appl. Mater. Interfaces

119

115

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Highly sensitive and selective DNA detection plays a central role in many fields of research and various assay platforms have been developed. Compared to homogeneous DNA detection, surface immobilized probes allow washing steps and signal amplification to give higher sensitivity. Previously research was focused on developing glass or gold based surfaces for DNA immobilization, we herein report hydrogel immobilized DNA. Specifically, acrydite-modified DNA was covalently functionalized to the polyacrylamide hydrogel during gel formation. There are several advantages of these DNAfunctionalized monolithic hydrogels. First, they can be easily handled in a way similar to that in homogeneous assays. Second, they have a low optical background where in combination with DNAfunctionalized gold nanoparticles, even ~0.1 nM target DNA can be visually detected. By using the attached gold nanoparticles to catalyze the reduction of Ag + , as low as 1 pM target DNA can be detected. The gels can be regenerated by a simple thermal treatment and the regenerated gels perform similarly compared to freshly prepared ones. The amount of gold nanoparticles adsorbed through DNA hybridization decreases with increasing gel percentage. Other parameters including DNA concentration, DNA sequence, ionic strength of the solution and temperature have also been systematically characterized in this study.

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“…Visual detection of cocaine was performed using a DNA modified linear polyacrylamide polymer, which was crosslinked with a cocaine aptamer and loaded with Au-NPs. In presence of cocaine, the hydrogel dissolves and Au-NPs are released in solution, turning its color from colorless to red and thus allowing its detection (224).…”

Section: Metal Nanoparticles For Biomedical Applicationsmentioning

confidence: 99%

Properties and Customization of Sensor Materials for Biomedical Applications

Zuliani¹,

Curto²,

Matzeu³

et al. 2014

Comprehensive Materials Processing

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An Aptamer Cross‐Linked Hydrogel as a Colorimetric Platform for Visual Detection

Abstract: US NIH ; China National Scientific Foundation of China [20805038, 20620130427]; National Basic Research Program of China [2007CB935603, 2010CB732402, 2009ZX10004-312]; ACS Division of Analytical Chemistr

Cited by 333 publications

References 63 publications

Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine

Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine

DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection

Properties and Customization of Sensor Materials for Biomedical Applications

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