An aptamer-based immunoassay in microchannels of a portable analyzer for detection of microcystin-leucine-arginine

Xiang, An; Li, Xiaoying; Ren, Feng-ling; Zang, Liuqin; Wang, Qin; Ju, Zhang; Lu, Zifan; Guo, Yanhai

doi:10.1016/j.talanta.2014.07.008

Cited by 17 publications

(6 citation statements)

References 31 publications

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“…[127] In comparison to Abs, better specificity and stability with MC-LR can be achieved with aptamers. [128] Aptamer-based biosensors have also been developed, a brief summary has been presented in the work of Zhang et al [129] These types of biosensors utilize the change in electrochemical properties of the electrodes to measure the concentration of MC-LR. This property has been utilized by Lin et al [116] to develop a label-free aptamer-based electrochemical impedance as recognition element has been developed for ultrasensitive detection of MC-LR.…”

Section: Protein Phosphate Inhibition Assaymentioning

confidence: 99%

Recent developments in the methods of quantitative analysis of microcystins

Kumar

Rautela

Kesari

et al. 2020

J Biochem & Molecular Tox

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Cyanotoxins are produced by the toxic cyanobacterial species present in algal blooms formed in water bodies due to nutrient over-enrichment by human influences and natural environmental conditions. Extensive studies are available on the most widely encountered cyanotoxins, microcystins (MCs) in fresh and brackish water bodies. MC contaminated water poses severe risks to human health, environmental sustainability, and aquatic life. Therefore, commonly occurring MCs should be monitored. Occasionally, detection and quantification of these toxins are difficult due to the unavailability of pure standards. Enzymatic, immunological assays, and analytical techniques like protein phosphatase inhibition assay, enzymelinked immunosorbent assay, high-performance liquid chromatography, liquid chromatography-mass spectrometry, and biosensors are used for their detection and quantification. There is no single method for the detection of all the different types of MCs; therefore, various techniques are often combined to yield reliable results. Biosensor development offered a problem-solving approach in the detection of MCs due to their high accuracy, sensitivity, rapid response, and portability. In this review, an endeavor has been made to uncover emerging techniques used for the detection and quantification of the MCs.

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Section: Protein Phosphate Inhibition Assaymentioning

confidence: 99%

Recent developments in the methods of quantitative analysis of microcystins

Kumar

Rautela

Kesari

et al. 2020

J Biochem & Molecular Tox

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“…One of them is microcystin-LR, a cyclic heptapeptide with a molecular mass of nearly 1000 Da. A sandwich assay combining an HRP (horse radish peroxidase)-labeled antibody and an aptamer (Xiang et al, 2014 ) as well as two aptamer-based electrochemical sensors managed to detect microcystin-LR in spiked water (Lin et al, 2013 ) or even fish samples (Eissa et al, 2014 ). A different, colorimetric sensor needed only 5 min analysis time (Wang F. et al, 2015 ), qualifying it for on-site usage.…”

Section: Small Molecule Aptasensorsmentioning

confidence: 99%

Selection and Biosensor Application of Aptamers for Small Molecules

2016

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Small molecules play a major role in the human body and as drugs, toxins, and chemicals. Tools to detect and quantify them are therefore in high demand. This review will give an overview about aptamers interacting with small molecules and their selection. We discuss the current state of the field, including advantages as well as problems associated with their use and possible solutions to tackle these. We then discuss different kinds of small molecule aptamer-based sensors described in literature and their applications, ranging from detecting drinking water contaminations to RNA imaging.

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“…The enzyme then hydrolyzes the urea (pH changes) or sucrose (glucose increases), these concentration-dependent changes can easily be measured using portable pH meters or glucometers [ 61 , 62 , 63 ]. In addition, other portable aptasensors using smartphones as readout [ 64 , 65 ], optic fiber technology [ 66 ], and microchannels coupled with portable analyzer [ 67 ] have also been developed for on-site monitoring the pollutants in water samples in recent years.…”

Section: Aptasensors and Their Applications For Detecting Low Molementioning

confidence: 99%

“…In this aptasensor, LOD was 0.3 μg·L −1 . A high specificity for MC-LR was detected against MC-LA, MC-YR, or nodularin-R and a good recovery of 94.5% to 112.7% was calculated in two spiked environmental samples [ 67 ]. In addition, AuNF/AgNPs-based aptasensor using SERS sensing platform were fabricated for detecting MC-LR.…”

Section: Aptasensors and Their Applications For Detecting Low Molementioning

confidence: 99%

Practical Application of Aptamer-Based Biosensors in Detection of Low Molecular Weight Pollutants in Water Sources

et al. 2018

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Water pollution has become one of the leading causes of human health problems. Low molecular weight pollutants, even at trace concentrations in water sources, have aroused global attention due to their toxicity after long-time exposure. There is an increased demand for appropriate methods to detect these pollutants in aquatic systems. Aptamers, single-stranded DNA or RNA, have high affinity and specificity to each of their target molecule, similar to antigen-antibody interaction. Aptamers can be selected using a method called Systematic Evolution of Ligands by EXponential enrichment (SELEX). Recent years we have witnessed great progress in developing aptamer selection and aptamer-based sensors for low molecular weight pollutants in water sources, such as tap water, seawater, lake water, river water, as well as wastewater and its effluents. This review provides an overview of aptamer-based methods as a novel approach for detecting low molecular weight pollutants in water sources.

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An aptamer-based immunoassay in microchannels of a portable analyzer for detection of microcystin-leucine-arginine

Cited by 17 publications

References 31 publications

Recent developments in the methods of quantitative analysis of microcystins

Recent developments in the methods of quantitative analysis of microcystins

Selection and Biosensor Application of Aptamers for Small Molecules

Practical Application of Aptamer-Based Biosensors in Detection of Low Molecular Weight Pollutants in Water Sources

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