An approach towards method development for untargeted urinary metabolite profiling in metabonomic research using UPLC/QToF MS

Urine and plasma metabonomics coupled with UHPLC-QTOF/MS and multivariate data analysis on potential biomarkers in anemia and hematinic effects of herb pair Gui-Hong

“…Analytical techniques such as nuclear magnetic resonance, gas chromatography-mass sp Q3 ectrometry a Q4 nd ultraperformance liquid chromatography-mass spectrometry (UHPLC-MS) are now generally applied in metabonomic studies (Dunn et al, 2005;Zhao et al, 2012aZhao et al, , 2012bZhao et al, , 2012c. Among the analytical techniques in metabonomic research, UHPLC-MS is recognized as one of the best analytical techniques in selectivity, sensitivity, and reproducibility (Theodoridis et al, 2008;Zhao et al, 2012aZhao et al, , 2012bZhao et al, , 2012cWong et al, 2008).…”

Metabonomic study of biochemical changes in the serum of type 2 diabetes mellitus patients after the treatment of metformin hydrochloride

“…UPLC featured by small particles of 1.7 m, has enabled better chromatographic peak resolution and increased speed and sensitivity to be obtained for complex mixture separation compared to HPLC. There have been an increasing number of applications of UPLC/MS to the analysis of biological fluids in the field of metabonomics [22][23][24][25][26][27][28].…”

Effect of mitiglinide on Streptozotocin-induced experimental type 2 diabetic rats: A urinary metabonomics study based on ultra-performance liquid chromatography–tandem mass spectrometry

“…UPLC featured by small particles, has enabled better chromatographic peak resolution and increased speed and sensitivity to be obtained for complex mixture separation compared to HPLC. There have been an increasing number of applications of UPLC/MS to the analysis of biological fluids in the field of metabonomics [23][24][25][26][27][28][29].…”