An approach to on-line electrospray mass spectrometric detection of polypeptide antibiotics of enramycin for high-speed counter-current chromatographic separation

Inoue, Kōichi; Hattori, Yasuko; Hino, Tomoaki; Oka, Hisao

doi:10.1016/j.jpba.2009.11.010

Cited by 25 publications

(14 citation statements)

References 16 publications

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“…No cross‐resistance with existing antibiotics was observed (Higashide et al, ; Kawakami, Nagai, Fujii, & Mitsuhashi, ). Standard grade enduracidin consists of enduracidin A and B (Inoue, Hattori, Hino, & Oka, ; Nogami et al, ) and is used as a food additive for pigs and chickens to protect them from necrotic enteritis.…”

Section: Antimicrobial Peptides Active Against Archaeamentioning

confidence: 99%

“…No crossresistance with existing antibiotics was observed (Higashide et al, 1968;Kawakami, Nagai, Fujii, & Mitsuhashi, 1971). Standard grade enduracidin consists of enduracidin A and B (Inoue, Hattori, Hino, & Oka, 2010;Nogami et al, 1984) and is used as a food additive for pigs and chickens to protect them from necrotic enteritis. Enduracidin has two antiparallel β-strands (Castiglione, Marazzi, Meli, & Colombo, 2005;Fang et al, 2006) and binds to lipid II (in a ratio of 2:1) resulting in the deactivation of PBP 1b enzyme known to play an important role in peptidoglycan biosynthesis.…”

Section: Enduracidinmentioning

confidence: 99%

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A review on comparative mechanistic studies of antimicrobial peptides against archaea

Varnava

Ronimus

Sarojini

2017

Biotech & Bioengineering

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Archaea was until recently considered as a third domain of life in addition to bacteria and eukarya but recent studies support the existence of only two superphyla (bacteria and archaea). The fundamental differences between archaeal, bacterial, and eukaryal cells are probably the main reasons for the comparatively lower susceptibility of archaeal strains to current antimicrobial agents. The possible emerging pathogenicity of archaea and the role of archaeal methanogens in methane emissions, a potent greenhouse gas, has led many researchers to examine the sensitivity patterns of archaea and make attempts to find agents that have significant anti-archaeal activity. Even though antimicrobial peptides (AMPs) are well known with several published reviews concerning their mode of action against bacteria and eukarya, to our knowledge, to date no reviews are available that focus on the action of these peptides against archaea. Herein, we present a review on all the peptides that have been tested against archaea. In addition, in an attempt to shed more light on possible future work that needs to be performed we have included a brief overview of the chemical characteristics, spectrum of activity, and the known mechanism of action of each of these peptides against bacteria and/or fungi. We also discuss the nature of and key physiological differences between Archaea, Bacteria, and Eukarya that are relevant to the development of anti-archaeal peptides. Despite our relatively limited knowledge about archaea, available data suggest that AMPs have an even broader spectrum of activity than currently recognized.

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Section: Antimicrobial Peptides Active Against Archaeamentioning

confidence: 99%

Section: Enduracidinmentioning

confidence: 99%

A review on comparative mechanistic studies of antimicrobial peptides against archaea

Varnava

Ronimus

Sarojini

2017

Biotech & Bioengineering

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“…It has also proved useful and possible to combine analytical instrument with CCC on line, such as MS, UV‐visible, FTIR, optical rotation dispersion (ORD) spectrometers and ELSD, pH meter, and so on. More and more on‐line technical and hardware problems have gradually been resolved and several applications on separation and purification of various natural products have been reported . One interesting prospect is CCC will tend toward more integrated systems, for example CPC–HPLC–DAD–MS combining separation, analysis, and identification in an on‐line single step .…”

Section: Prospectsmentioning

confidence: 99%

Developments in high-speed countercurrent chromatography and its applications in the separation of terpenoids and saponins

et al. 2016

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High-speed countercurrent chromatography is a liquid-liquid separation chromatographic technique, which has the unique feature of eliminating irreversible adsorption using liquid support medium, and is widely used in research and development of traditional Chinese medicine, biochemistry, food, environment analysis, and so on. In this review, some new developments of countercurrent chromatography, for instance cross-axis countercurrent chromatography, dual countercurrent chromatography, foam countercurrent chromatography, and pH-zone-refining countercurrent chromatography are presented. Furthermore, the research and progress in high-speed countercurrent chromatography techniques and its application in the separation and purification of terpenoids and saponins are reviewed.

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“…The utilization of LC–CCC–MS expanded the application of CCC technique into lower concentration compounds. In total ion current (LC–CCC–TIC) chromatogram, more peaks could be detected and in turn separated . Still, the peak areas of some small peaks are unable to be acquired because of poor resolution and high background noise in the LC–TIC chromatogram, making determination of the K C values difficult.…”

Section: Introductionmentioning

confidence: 99%

Elution–extrusion countercurrent chromatography separation of six pairs of isomeric iridoids from Cornus officinalis Sieb. et Zucc. guided by ion current extraction in mass spectrometry

Liang

Sun

2018

J of Separation Science

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A mass spectrometry-guided elution-extrusion countercurrent chromatography protocol was developed to separate chemical components from Cornus officinalis Sieb. et Zucc. In this study, ion current extraction, a mass spectrometry-based data postacquisition method, was utilized to boost the separation power and scope of countercurrent chromatography technique. As a peak repicking and denoising tool, ion current extraction was carried out to process the liquid chromatography with mass spectrometry and the countercurrent chromatography with mass spectrometry data. So the target components were reacquired in the created extracted ion current patterns with enhanced responses and diminished background noise, which facilitated the distribution constant determination (by liquid chromatography with extracted ion current) and the targets fractionation (by countercurrent chromatography with extracted ion current). Under the guidance of the extracted ion currents of the target components and with the support of elution-extrusion mode in the countercurrent chromatography separation, six pairs of minor iridoid isomers were obtained in shortened experimental duration. Besides, a reciprocal shifted symmetry plot was established to represent the elution-extrusion countercurrent chromatography chromatogram. The results demonstrated the capability of the ion current extraction-guided elution-extrusion countercurrent chromatography protocol in discovery, analysis, and fractionation of low-concentration and structurally similar chemicals from a complicated sample.

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An approach to on-line electrospray mass spectrometric detection of polypeptide antibiotics of enramycin for high-speed counter-current chromatographic separation

Cited by 25 publications

References 16 publications

A review on comparative mechanistic studies of antimicrobial peptides against archaea

A review on comparative mechanistic studies of antimicrobial peptides against archaea

Developments in high-speed countercurrent chromatography and its applications in the separation of terpenoids and saponins

Elution–extrusion countercurrent chromatography separation of six pairs of isomeric iridoids from Cornus officinalis Sieb. et Zucc. guided by ion current extraction in mass spectrometry

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