An Approach to Identify SNPs in the Gene Encoding Acetyl-CoA Acetyltransferase-2 (ACAT-2) and Their Proposed Role in Metabolic Processes in Pig

Sodhi, Simrinder Singh; Ghosh, Mrinmoy; Song, Ki Duk; Sharma, Neelesh; Kim, Jeong Hyun; Kim, Nameun; Lee, Sung‐Jin; Kang, Chul Woong; Oh, Sung Jong; Jeong, Dong Kee

doi:10.1371/journal.pone.0102432

Cited by 20 publications

(18 citation statements)

References 37 publications

(40 reference statements)

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“…Furthermore, in the F group only a percentage of fatty acids could be entering β-oxidation without altering the VLCAD and ACAA2 expression, and probably the rest could be metabolized in fatty acid intermediates, such as diacylglycerol and ceramides, generating mitochondrial stress in the cardiomyocyte, which could contribute to expression changes of the rest of the proteins evaluated in this study [37]. Moreover, curcumin supplemented with the HFD increased ACAA2 expression, which suggests that fatty acids oxidation was not inhibited and this could be a beneficial effect of curcumin given that if the activity of ACAA2 is suppressed, the ability of mitochondria to oxidize fatty acids is limited [38]. In addition, PPARα was overexpressed when the curcumin was administrated together with HFD, this overexpression could upward regulate ACAA2, since its expression is under the transcriptional control of PPARα.…”

Section: Discussionmentioning

confidence: 85%

Curcumin ameliorates protein expression changes involved in mitochondrial fatty acids metabolism in heart of mice fed a high-fructose diet

Meléndez-Salcido¹,

Vargas-Ortiz²,

Silva-Gaona³

et al. 2020

Preprint

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Background: It has been proposed that curcumin modulates the gene expression of different signaling pathways, improve the fatty acids metabolism and exerts a potential beneficial effect on cardiometabolic disease, but this has not been thoroughly demonstrated. In the present study, we evaluated the effect of curcumin upon the expression of PPARα, CPT1, MCAD, VLCAD and ACAA2 in heart of mice fed a high-fructose diet (HFD).Methods: Twenty-four mice C57BL/6 were divided into four groups (n=6) and treated for 15 weeks. Control group (C) received standard diet (SD), Curcumin group (C+Cur), Fructose group (F) and Fructose with Curcumin group (F+Cur). The groups were treated with 0.75% w/w curcumin mixed in the SD and 30% w/v fructose in water, respectively. Heart proteins expression were analyzed by Western Blot.Results: Curcumin supplementation increased PPARα and ACAA2 expression and decreased CPT1 and MCAD expression in heart of mice fed a HFD. However, it did not modify the VLCAD expression.Conclusions: Curcumin regulated PPARα, CPT1 and MCAD expression and increased ACAA2 expression; suggesting a therapeutic potential in the prevention of alterations in mitochondrial fatty acids metabolism in heart of mice fed a HFD.

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Section: Discussionmentioning

confidence: 85%

Curcumin ameliorates protein expression changes involved in mitochondrial fatty acids metabolism in heart of mice fed a high-fructose diet

Meléndez-Salcido¹,

Vargas-Ortiz²,

Silva-Gaona³

et al. 2020

Preprint

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“…Further analysis found that among the 21 proteins quanti ed, ten proteins that regulate fat deposition have major variations in expression between leg muscles and breast muscles. For example, Acetyl CoA Acyltransferase 2 (AcetylCoAacyltransferase 2, ACAA2) is a key enzyme for fatty acid oxidation, widely distributed in mammalian and bird tissues and cytoplasm, and plays a vital role in the metabolism of fatty acids (Sodhi et al 2014), and regulates intermuscular fat content (Miltiadou et al 2017). Hence, this gene is considered a candidate for detecting intermuscular fat content (Miltiadou et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Proteomics Analysis and Identification of Critical Proteins and Network Interactions That Regulate the Specific Deposition of IMF of Jingyuan Chicken

Huang

Zhang

et al. 2020

Preprint

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Background:Improving the production efficiency and quality of broiler chickens has become a hot topic in molecular breeding of the broiler industry. There are several factors, among which intramuscular fat (IMF) content is one of the essential factors determining chicken quality. The chicken carcass weight of 40-50% consisted of the leg muscles and breast muscles, and the research on chicken meat quality was performed on that part. Jingyuan chicken is one of the high-quality local chicken species in China. However, the molecular mechanism of IMF specific deposition in different parts of Jingyuan chicken is still unclear. In order to screen and identify the proteins that regulate IMF specific deposition in different parts of Jingyuan chicken and analyze network interaction, the present study selected 180-day-old Jingyuan chicken as the research material, using proteomic techniques and PRM experimental methods to carry out basic research on leg muscle and breast muscle. Results:Three key signaling pathways and ten essential functional proteins regulating IMF specific deposition of Jingyuan chicken were identified, while the molecular mechanism of the deposition of proteins was explained, and analyzed the network interaction between these key functional proteins. Conclusions:The results of this study are of great scientific value and theoretical significance not only for improving chicken quality but also for molecular breeding of high-quality local chickens.

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“…Therefore, we used sequence- and structure-based approaches to investigate the most salient polymorphisms in MYH1 in JNP and Berkshire breeds. Such missense variants are considered to be potentially critical for the function and structure of a protein, and thus they might provide markers for the selection of specific phenotypic traits [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

An integrated in silico approach for functional and structural impact of non- synonymous SNPs in the MYH1 gene in Jeju Native Pigs

Ghosh¹,

Sodhi²,

Sharma³

et al. 2016

BMC Genet

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BackgroundThis study was performed to identify the non- synonymous polymorphisms in the myosin heavy chain 1 gene (MYH1) association with skeletal muscle development in economically important Jeju Native Pig (JNP) and Berkshire breeds. Herein, we present an in silico analysis, with a focus on (a) in silico approaches to predict the functional effect of non-synonymous SNP (nsSNP) in MYH1 on growth, and (b) molecular docking and dynamic simulation of MYH1 to predict the effects of those nsSNP on protein-protein association.ResultsThe NextGENe (V 2.3.4.) tool was used to identify the variants in MYH1 from JNP and Berkshire using RNA seq. Gene ontology analysis of MYH1 revealed significant association with muscle contraction and muscle organ development. The 95 % confidence intervals clearly indicate that the mRNA expression of MYH1 is significantly higher in the Berkshire longissimus dorsi muscle samples than JNP breed. Concordant in silico analysis of MYH1, the open-source software tools identified 4 potential nsSNP (L884T, K972C, N981G, and Q1285C) in JNP and 1 nsSNP (H973G) in Berkshire pigs. Moreover, protein-protein interactions were studied to investigate the effect of MYH1 mutations on association with hub proteins, and MYH1 was found to be closely associated with the protein myosin light chain, phosphorylatable, fast skeletal muscle MYLPF. The results of molecular docking studies on MYH1 (native and 4 mutants) and MYLFP demonstrated that the native complex showed higher electrostatic energy (−466.5 Kcal mol−1), van der Walls energy (−87.3 Kcal mol−1), and interaction energy (−835.7 Kcal mol−1) than the mutant complexes. Furthermore, the molecular dynamic simulation revealed that the native complex yielded a higher root-mean-square deviation (0.2–0.55 nm) and lower root-mean-square fluctuation (approximately 0.08–0.3 nm) as compared to the mutant complexes.ConclusionsThe results suggest that the variants at L884T, K972C, N981G, and Q1285C in MYH1 in JNP might represent a cause for the poor growth performance for this breed. This study is a pioneering in-depth in silico analysis of polymorphic MYH1 and will serve as a valuable resource for further targeted molecular diagnosis and population-based studies conducted for improving the growth performance of JNP.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-016-0341-1) contains supplementary material, which is available to authorized users.

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An Approach to Identify SNPs in the Gene Encoding Acetyl-CoA Acetyltransferase-2 (ACAT-2) and Their Proposed Role in Metabolic Processes in Pig

Cited by 20 publications

References 37 publications

Curcumin ameliorates protein expression changes involved in mitochondrial fatty acids metabolism in heart of mice fed a high-fructose diet

Curcumin ameliorates protein expression changes involved in mitochondrial fatty acids metabolism in heart of mice fed a high-fructose diet

Proteomics Analysis and Identification of Critical Proteins and Network Interactions That Regulate the Specific Deposition of IMF of Jingyuan Chicken

An integrated in silico approach for functional and structural impact of non- synonymous SNPs in the MYH1 gene in Jeju Native Pigs

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