Infrared radiation is closely associated with skin diseases, such as photodermatosis and wrinkle formation, and erythema. 1,2 Infrared-A (IRA, 700-1400 nm) radiation constitutes approximately 30% of solar infrared radiation, which can penetrate down to the hypodermis and affect various types of cells in the skin. 3,4 However, the effects of IRA irradiation on skin cells are controversial, probably because of differences in IRA exposure conditions, such as the intensity of irradiation, exposure period, and temperature. 5,6 In addition, heat stresses during IRA irradiation on skin can induce various harmful effects, including the generation of reactive oxygen species (ROS) and apoptotic signals, making it difficult to understand the biological effects of IRA irradiation alone. 7,8 Hence, it is necessary to study the cellular effects of IRA irradiation alone under defined conditions of duration, frequency, and dose of IRA irradiation, as well as