An Approach for Rapid Manufacture and Qualification of a Single‐Use Bioreactor Prototype

Kaiser, Stefan

doi:10.1002/9781119477891.ch20

Cited by 2 publications

(2 citation statements)

References 35 publications

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“…However, since sterile bioreactors were not strictly necessary for the intended microbial proof of concept cultivation, unsterilized bioreactors were used for the E. coli fed-batch cultivation. 4), the open pipe sparger used for the Escherichia coli cultivations (5), the overlay aeration port ( 6), the baffle-sparger ( 7), the levitating impeller (8), the pH and dissolved oxygen (DO) spots ( 9), the submerged sampling port (10) and the biomass sensor (11). From the bottom to the top plate, the reactor is approximately 100 mm tall, and the middle section has an outer diameter of 53 mm.…”

Section: Bioreactor Design and Assemblymentioning

confidence: 99%

“…The same constraints extend to single-use bioreactors, since specialized molds and tools need to be created, rendering iterative design evaluation both slow and expensive. A potential solution to this challenge is additive manufacturing (AM), which has already been employed in rapid prototyping approaches to expedite product development [8]. However, only employing AM in the development phase does not leverage its full potential.…”

Section: Introductionmentioning

confidence: 99%

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3D Printed, Single-Use Bioreactor with Integrated Inline Sensors for Microbial and Mammalian Cell Cultivation—A Case Study

Schneider,

Seidel,

Seefeldt

et al. 2023

Processes

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The development of upstream bioprocesses necessitates small, instrumented bioreactors for investigating and optimizing production processes in a cost-effective manner. Due to advances in both the equipment and the materials used in additive manufacturing, 3D printing of customized bioreactors is now in the realm of possibilities. In this study, a small-scale 3D printed bioreactor suitable for mammalian and microbial cultivations was developed, featuring a working volume of 90 mL, inline pH and dissolved oxygen probes and a levitating magnetic stirrer. Aeration channels and a sampling port were printed directly into the vessel walls. Additionally, the vessel was equipped with a 3D printed customizable optical biomass-sensor. The bioreactor’s performance was evaluated through technical characterization and proof of concept cultivations, demonstrating that mixing time and oxygen mass transfer were sufficient for cultivating mammalian as well as microbial cells at high cell densities. Specifically, an Escherichia coli fed-batch cultivation achieved a maximum OD600 of 204. Furthermore, a fed-batch cultivation of an IgG antibody-producing Chinese hamster ovary cell line reached a peak viable cell density of 10.2 × 106 cells mL−1 and a maximum product titer of 2.75 g L−1. Using a three-parameter fit, the inline biomass signal could be correlated to the corresponding offline values with satisfactory accuracy, making it possible to monitor cell growth in real-time.

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Section: Bioreactor Design and Assemblymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%