An approach for genogrouping of Japanese isolates of aquabirnaviruses in a new genogroup, VII, based on the VP2/NS junction region

Abstract

Aquabirnaviruses, represented by Infectious pancreatic necrosis virus (IPNV), have been isolated from epizootics in salmonids and a variety of aquatic animals in the world; six genogroups of aquabirnaviruses have been identified. In comparisons of nucleotide sequences of the VP2/NS junction region, maximum nucleotide diversities of 30?8 % were observed among 93 worldwide aquabirnavirus isolates. A phylogenetic tree revealed the existence of a new genogroup, VII, for Japanese aquabirnavirus isolates from marine fish and molluscan shellfish. Nucleotide diversities between genogroups VII and I-VI were 18?7 % or greater. At the nucleotide level, Japanese IPNV isolates from epizootics in salmonids were nearly identical to a genogroup I strain from the USA or Canada. It is suggested that Japanese IPNV isolates belonging to genogroup I were originally introduced from North American sources, whereas Japanese aquabirnavirus isolates of genogroup VII were from marine aquatic animals indigenous to Japan.

Genotipificacion y relacion hospedador especifica del virus de la necrosis pancreatica infecciosa en Chile

“…La primera clasificación filogenética fue propuesta por Blake et al (2001), donde se describió la presencia de 6 genogrupos, compuestos por diferentes cepas de origen europeo, americano y asiático. Posteriormente, Nishizawa et al (2005) revelaron la existencia de un séptimo genogrupo integrado únicamente por cepas japonesas, y recientemente en Australia se aisló un nuevo Aquabirnavirus desde trucha arcoíris, que fue clasificado en un octavo genogrupo (McCowan et al, 2015;Mohr et al, 2015). En Chile, los virus IPN que han sido caracterizados genéticamente pertenecen a los Genogrupos 1 y 5, i.e., norteamericanos y europeos, respectivamente (Eissler et al, 2011;Mutoloki & Evensen, 2011;Calleja et al, 2012;Tapia et al, 2015;Jorquera et al, 2016), dentro de los cuales forman sus propios subtipos o subgrupos (Tapia et al, 2015).…”

Detection and Genetic Analysis of Aquabirnaviruses in Subclinically Infected Aquarium Fish

“…nih.gov/blast/Blast.cgi), and these products were used for the phylogenetic analysis of the ABV isolates because of the diversity present among the isolates. 13,20 The variable region of the VP2/NS (134 nucleotides [nt], from nt 1,517 to nt 1,650) was aligned and compared with previously reported data for the ABV isolates. Multiple alignment algorithms from the ClustalX f method were analyzed with the Molecular Evolutionary Genetics Analysis (MEGA) program (version 4.0) g using the neighbor-joining method.…”

“…The phylogenetic analysis of nested RT-PCR products obtained from these samples placed them within the ABV genogroup VII, which also contains the MABV subgroup. 20,27 Based on previous reports, isolates of MABV have been limited only to Korea and Japan. 13,20,23 Isolates of ABV described to date are summarized in Table 1.…”

“…6 The junction region of the VP2/NS was previously used for the phylogenetic analysis of the ABV isolates. 20 The ABV can be divided in to 7 genogroups; more specifically, IPNVs belong to the genogroups I-VI, and MABV belongs to the genogroup VII. 20 Segment B encodes VP1, which is the RNA-dependent RNA polymerase associated with virions.…”

“…20 The ABV can be divided in to 7 genogroups; more specifically, IPNVs belong to the genogroups I-VI, and MABV belongs to the genogroup VII. 20 Segment B encodes VP1, which is the RNA-dependent RNA polymerase associated with virions. 5 In Korea, IPNV has been isolated from cultured chum salmon (Oncorhynchus keta) and goldfish (Carassius auratus).…”