Measurements of radiation emitted by the atmosphere of the earth in an infrared absorption band of water vapor, obtained with satellite‐borne instrumentation, contain information on atmospheric relative humidity. Two methods are developed for estimating tropospheric relative humidity profiles from infrared spectral measurements for which the spectral resolution elements are narrow in comparison with the total width of the absorption band but wide in comparison with a single absorption line. The methods, which are essentially complementary, consist of a direct estimation technique, which requires a minimum of a priori knowledge of the behavior of the relative humidity profile, and a statistical estimation technique, which can make full use of a knowledge of the statistics of tropospheric humidities in situations where such knowledge exists. An analysis of the propagation of errors in the measured spectral intensities indicates that meaningful estimates should be obtained from the 6.3‐μ water vapor band in the presence of realistic instrumental noise for most types of atmospheres. One exception is the polar winter atmosphere, where strong error propagation occurs because of the behavior of the temperature profile. An examination of the effects on the inferred relative humidity profile of errors in the temperature profile employed in the estimation reveals that the relative humidities inferred in the lowest layers of the troposphere are extremely sensitive to errors in the surface temperature, which may prove to be the limiting factor in obtaining complete relative humidity profiles. Examples of applications of the techniques to synthetic data from model atmospheres and to real data obtained with a balloon‐borne infrared interferometer spectrometer are given.