An apple CIPK protein kinase targets a novel residue of AREB transcription factor for ABA‐dependent phosphorylation

Ma, Qi-Jun; Sun, Ming; Lü, Jing; Liu, Ya‐Jing; You, Chun‐Xiang; Hao, Yu‐Jin

doi:10.1111/pce.13013

Cited by 67 publications

(46 citation statements)

References 67 publications

(116 reference statements)

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“…Sixty-eight MdDREB genes have been classified into six subgroups against the entire genome of apple, of which MdDREB2A and MdDREB2B (called MDP0000147009 and MDP0000153866 in their study) were identified and found they could be up-regulated under various abiotic stress treatments (Zhao et al, 2012). MdAREB1A and MdAREB1B (called MdAREB3.2 and MdAREB2 in their study) were identified and studied for their sensitivity to ABA in apple (Ma et al, 2017). MdRD22, MdRD29A and MdRD29B were also found to be responsive for drought or osmotic stress Shao et al, 2019).…”

Section: Which Can Act Directly On the M46re Cis-regulatory Elementmentioning

confidence: 99%

MdMYB46 could enhance salt and osmotic stress tolerance in apple by directly activating stress‐responsive signals

Chen

Song

Guo

et al. 2019

Plant Biotechnology Journal

143

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Summary To expand the cultivation area of apple (Malus×domestica Borkh.) and select resistant varieties by genetic engineering, it is necessary to clarify the mechanism of salt and osmotic stress tolerance in apple. The MdMYB46 transcription factor was identified, and the stress treatment test of MdMYB46‐overexpressing and MdMYB46‐RNAi apple lines indicated that MdMYB46 could enhance the salt and osmotic stress tolerance in apple. In transgenic Arabidopsis and apple, MdMYB46 promoted the biosynthesis of secondary cell wall and deposition of lignin by directly binding to the promoter of lignin biosynthesis‐related genes. To explore whether MdMYB46 could coordinate stress signal transduction pathways to cooperate with the formation of secondary walls to enhance the stress tolerance of plants, MdABRE1A, MdDREB2A and dehydration‐responsive genes MdRD22 and MdRD29A were screened out for their positive correlation with osmotic stress, salt stress and the transcriptional level of MdMYB46. The further verification test demonstrated that MdMYB46 could activate their transcription by directly binding to the promoters of these genes. The above results indicate that MdMYB46 could enhance the salt and osmotic stress tolerance in apple not only by activating secondary cell wall biosynthesis pathways, but also by directly activating stress‐responsive signals.

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Section: Which Can Act Directly On the M46re Cis-regulatory Elementmentioning

confidence: 99%

MdMYB46 could enhance salt and osmotic stress tolerance in apple by directly activating stress‐responsive signals

Chen

Song

Guo

et al. 2019

Plant Biotechnology Journal

143

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“…Our yeast two hybrid experiment showed that there was interaction between FtbZIP5 and the homologous protein FtSnRK2.6 of SnRK2.6, but their phosphorylation requires further verification. Similarly, in apple, MdAREB2 is phosphorylated by the SnRK2 homologous protein MdCIPK22 [46]. Previous studies have found an interaction between FtbZIP83 and FtSnRK2.6/2.3 [23].…”

Section: Discussionmentioning

confidence: 89%

Tartary Buckwheat Transcription Factor FtbZIP5, Regulated by FtSnRK2.6, Can Improve Salt/Drought Resistance in Transgenic Arabidopsis

Zhao

Wang

et al. 2020

IJMS

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bZIP transcription factors have been reported to be involved in many different biological processes in plants. The ABA (abscisic acid)-dependent AREB/ABF-SnRK2 pathway has been shown to play a key role in the response to osmotic stress in model plants. In this study, a novel bZIP gene, FtbZIP5, was isolated from tartary buckwheat, and its role in the response to drought and salt stress was characterized by transgenic Arabidopsis. We found that FtbZIP5 has transcriptional activation activity, which is located in the nucleus and specifically binds to ABRE elements. It can be induced by exposure to PEG6000, salt and ABA in tartary buckwheat. The ectopic expression of FtbZIP5 reduced the sensitivity of transgenic plants to drought and high salt levels and reduced the oxidative damage in plants by regulating the antioxidant system at a physiological level. In addition, we found that, under drought and salt stress, the expression levels of several ABA-dependent stress response genes (RD29A, RD29B, RAB18, RD26, RD20 and COR15) in the transgenic plants increased significantly compared with their expression levels in the wild type plants. Ectopic expression of FtbZIP5 in Arabidopsis can partially complement the function of the ABA-insensitive mutant abi5-1 (abscisic acid-insensitive 5-1). Moreover, we screened FtSnRK2.6, which might phosphorylate FtbZIP5, in a yeast two-hybrid experiment. Taken together, these results suggest that FtbZIP5, as a positive regulator, mediates plant tolerance to salt and drought through ABA-dependent signaling pathways.

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“…For CO‐IP analysis, precipitates were further analysed by SDS‐PAGE and Western Blotting as described by Ma et al . (). The proteins from crude lysates (input) were detected with anti‐Myc antibody, and the IPed proteins (output) were detected with anti‐HA antibody.…”

Section: Methodsmentioning

confidence: 97%

“…After brief centrifugation, four washing steps followed, after which loading buffer was added to the precipitates and boiled. For CO-IP analysis, precipitates were further analysed by SDS-PAGE and Western Blotting as described by Ma et al (2017b). The proteins from crude lysates (input) were detected with anti-Myc antibody, and the IPed proteins (output) were detected with anti-HA antibody.…”

Section: Co-immunoprecipitation Proceduresmentioning

confidence: 99%

“…In apple, it is found that the MdAREB2‐MdSUT2.2 (formly named MdSUT2) regulon plays an important role in ABA‐induced sugar accumulation in apple (Ma et al ., ), and that a protein kinase MdCIPK22 interacts with and phosphorylates MdABRE2 to regulate its transcription activity in response to ABA signal (Ma et al ., ). This study provides evidence for MdCIPK22 as a positive regulator of the sucrose transporter MdSUT2.2.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

An apple sucrose transporter MdSUT2.2 is a phosphorylation target for protein kinase MdCIPK22 in response to drought

Sun

Lü

et al. 2018

Plant Biotechnology Journal

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Sugars increase with drought stress in plants and accumulate in the vacuole. However, the exact molecular mechanism underlying this process is not clear yet. In this study, protein interaction and phosphorylation experiments were conducted for sucrose transporter and CIPK kinase in apple. The specific phosphorylation site of sucrose transporter was identified with mass spectrometry. Transgenic analyses were performed to characterize their biological function. It was found that overexpression of sucrose transporter gene MdSUT2.2 in apple plants promoted sugar accumulation and drought tolerance. MdSUT2.2 protein was phosphorylated at Ser site in response to drought. A DUALmembrane system using MdSUT2.2 as bait through an apple cDNA library got a protein kinase MdCIPK22. Bimolecular fluorescence complementary (BiFC), pull-down and co-immunoprecipitation (Co-IP) assays further demonstrated that MdCIPK22 interacted with MdSUT2.2. A series of transgenic analysis showed that MdCIPK22 was required for the drought-induced phosphylation at Ser site of MdSUT2.2 protein, and that it enhanced the stability and transport activity of MdSUT2.2 protein. Finally, it was found that MdCIPK22 overexpression promoted sugar accumulation and improved drought tolerance in an MdSUT2.2-dependent manner in transgenic apple plants. MdCIPK22-MdSUT2.2 regulatory module shed light on the molecular mechanism by which plant accumulates sugars and enhances tolerance in response to drought stress.

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An apple CIPK protein kinase targets a novel residue of AREB transcription factor for ABA‐dependent phosphorylation

Cited by 67 publications

References 67 publications

MdMYB46 could enhance salt and osmotic stress tolerance in apple by directly activating stress‐responsive signals

MdMYB46 could enhance salt and osmotic stress tolerance in apple by directly activating stress‐responsive signals

Tartary Buckwheat Transcription Factor FtbZIP5, Regulated by FtSnRK2.6, Can Improve Salt/Drought Resistance in Transgenic Arabidopsis

An apple sucrose transporter MdSUT2.2 is a phosphorylation target for protein kinase MdCIPK22 in response to drought

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