An Apolipoprotein CIII-Derived Peptide, Hatktak, Activates Macromolecular Activators of Phagocytosis from Platelets (MAPPs)

Sakamoto, Haruhiko; Ueno, Masaki; Wu, Bin; Nagai, Y.; Matsumoto, Kouichi; Yamanaka, Takao; Tanaka, Sumiko

doi:10.5772/48173

Cited by 1 publication

(7 citation statements)

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“…In the previous study, confocal microscopic observations revealed that the reactions of anti‐HATKTAK antibody and anti‐CD61 antibodies overlap in platelets that are aggregated to each other. In this study, the reaction of anti‐HATKTAK antibody to platelets was confirmed by immunoelectron microscopy, in which the binding of anti‐HATKTAK IgG was observed on pseudopods of activated platelets through the action of anti‐rabbit IgG conjugated with colloidal gold parcticles.…”

Section: Discussionmentioning

confidence: 88%

“…Indirect ELISA revealed that anti‐HATKTAK rabbit antibody reacts with HATKTAK and KHATKTAK, but does not react with HATKTAKD and Apo CIII, even at high concentration (20 μg/well). In a previous study, we showed that anti‐HATKTAK IgG reacts strongly with S1‐K24 of Apo CIII, only weakly with A19‐K24 (ATKTAK), but not with HATKTAAK. These findings suggest that the positive reaction of this antibody in the tissue reflects the existence of Apo CIII‐derived peptides with the C‐terminal peptide sequence, HATKTAK.…”

Section: Discussionmentioning

confidence: 90%

“…In a previous study, we obtained evidence revealing that H18‐K24 of Apo CIII (HATKTAK) is cleaved from Apo CIII of HDL by the action of thrombin and activates MAPPs in platelets. The most abundant natural substrate of thrombin is fibrinogen .…”

Section: Discussionmentioning

confidence: 99%

“…A rabbit antibody against HATKTAK was raised by Sigma Aldrich Japan (Ishikari, Japan) in accordance with the method described in a previous report using keyhole limpet hemocyanin (KLH) as a carrier protein. Sera were obtained from the immunized rabbit before and after immunization.…”

Section: Methodsmentioning

confidence: 99%

“…Studies of MAPP production using platelet lysate revealed that some peptides from the macromolecular components can be a direct activator of MAPPs, and that the macromolecule belongs to the high density lipoproteins (HDL). The direct activator of MAPPs is HATKTAK (one‐letter representation of H18‐K24 of Apo CIII), which is liberated from Apo CIII of high‐density lipoprotein in platelets by the action of thrombin …”

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confidence: 99%

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An immunohistochemical study of human platelets using a rabbit antibody against H18‐K24 of apolipoprotein CIII (HATKTAK)

Yamanaka

Sakamoto

Nakagawa

et al. 2013

Pathology International

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H18-K24 of human apolipopotein CIII (Apo CIII) (HATKTAK) is an activator of the macromolecular activators of phagocytosis from platelets (MAPPs). Using a rabbit antibody against HATKTAK, we performed an immunohistochemical study of human platelets. Indirect ELISA showed that this antibody reacts with Apo CIII-derived peptides with a C-terminal of HATKTAK, but not with Apo CIII. Immunoelectron microscopy revealed that reaction of anti-HATKTAK antibody occurred in the pseudopods of activated platelets. In blood coagula produced from the peripheral blood and formalin-fixed after various incubation periods, reaction of this antibody with platelets appeared rapidly with a peak at 3 to 6 h of incubation, and then diminished gradually. Leukocytes in the blood coagula were stained strongly positive. In tissue sections, fresh thrombi and hemorrhages with slight fibrin formation revealed a positive response of platelets to anti-HATKTAK antibody, whereas older ones with leukocytic infiltration, fibrin formation and organization did not. In addition to platelets, endothelial cells and leukocytes were stained positive by anti-HATKTAK antibody. All of the positive reactions by anti-HATKTAK antibody disappeared or diminished by co-incubation with HATKTAK. In conclusion, the anti-HATKTAK antibody reveals platelets during the early phase of activation.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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