An AP2/EREBP-type transcription-factor gene from rice is cold-inducible and encodes a nuclear-localized protein

Chen, Jianquan; Dong, Yuanhong; Wang, Yujun; Liu, Qiang; Zhang, Jinsong; Chen, Shouyi

doi:10.1007/s00122-003-1346-5

Cited by 65 publications

(37 citation statements)

References 36 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upon bacterial root infection, de novo accumulation of active ERN3 presumably leads to the progressive repression of ERN1/ERN2 transcription activities. (Chen et al, 2003;Krizek and Sulli, 2006;Saleh et al, 2006). We observed that the ERN1 fusion protein is also associated with discrete nuclear bodies (Figure 7).…”

Section: Ern Factor Interplay May Coordinate Nf-elicited Enod Gene Acmentioning

confidence: 70%

AP2-ERF Transcription Factors Mediate Nod Factor–Dependent MtENOD11Activation in Root Hairs via a Novelcis-Regulatory Motif

et al. 2007

View full text Add to dashboard Cite

Rhizobium Nod factors (NFs) are specific lipochitooligosaccharides that activate host legume signaling pathways essential for initiating the nitrogen-fixing symbiotic association. This study describes the characterization of cis-regulatory elements and trans-interacting factors that regulate NF-dependent and epidermis-specific gene transcription in Medicago truncatula. Detailed analysis of the Mt ENOD11 promoter using deletion, mutation, and gain-of-function constructs has led to the identification of an NF-responsive regulatory unit (the NF box) sufficient to direct NF-elicited expression in root hairs. NF boxmediated expression requires a major GCC-like motif, which is also essential for the binding of root hair-specific nuclear factors. Yeast one-hybrid screening has identified three closely related AP2/ERF transcription factors (ERN1 to ERN3) that are able to bind specifically to the NF box. ERN1 is identical to an ERF-like factor identified recently. Expression analysis has revealed that ERN1 and ERN2 genes are upregulated in root hairs following NF treatment and that this activation requires a functional NFP gene. Transient expression assays in Nicotiana benthamiana have further shown that nucleus-targeted ERN1 and ERN2 factors activate NF box-containing reporters, whereas ERN3 represses ERN1/ERN2-dependent transcription activation. A model is proposed for the fine-tuning of NF-elicited gene transcription in root hairs involving the interplay between repressor and activator ERN factors.

show abstract

Section: Ern Factor Interplay May Coordinate Nf-elicited Enod Gene Acmentioning

confidence: 70%

AP2-ERF Transcription Factors Mediate Nod Factor–Dependent MtENOD11Activation in Root Hairs via a Novelcis-Regulatory Motif

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Thus, the expression of these cold-responsive genes was due, at least in part, to elevated levels of ABA. Nevertheless, recent eVort has proved that cold-induced gene expression can also proceed through at least one ABAindependent pathway (Chen et al 2003). The expression of OsCOIN was induced by exogenous ABA suggesting that the expression of the OsCOIN is ABA-dependent in rice (Fig.…”

Section: Figmentioning

confidence: 98%

Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice

Liu

Wang

et al. 2007

Planta

155

View full text Add to dashboard Cite

Rice (Oryza sativa L.) plant is sensitive to chilling, particularly at early stages of seedling development. Here a novel cold-inducible gene, designated OsCOIN (Oryza sativa cold-inducible), was isolated and characterized. Results showed that OsCOIN protein, a RING finger protein, was localized in both nuclear and cytoplasm membrane. OsCOIN is expressed in all rice organs and strongly induced by low temperature, ABA, salt and drought. Over-expression of OsCOIN in transgenic rice lines significantly enhanced their tolerance to cold, salt and drought, accompanied by an up-regulation of OsP5CS expression and an increase of cellular proline level.

show abstract

“…The recombinant yeast with two reporter genes grew in the selective medium plate, and its growth was completely inhibited by 10 mM 3-AT. The b-galactosidase activities were analyzed as described by Chen et al (2003).…”

Section: Dre-binding Activities Of the Dvdreb2a Proteinmentioning

confidence: 99%

Molecular cloning, expression profiling and trans-activation property studies of a DREB2-like gene from chrysanthemum (Dendranthema vestitum)

et al. 2008

View full text Add to dashboard Cite

Dehydration responsive element binding (DREB) proteins are important transcription factors in plant stress response and signal transduction. In this study, a DREB homolog gene, DvDREB2A, was isolated from chrysanthemum (Dendranthema vestitum) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. It contained an open reading frame (ORF) of 1,471 bp encoding 366 amino acid residues and was classified as a DREB2 subfamily member based on multiple sequence alignment. The predicted protein sequence contained a typical AP2/EREBP DNA-binding domain near the N-terminal region. In yeast one-hybrid analysis DvDREB2A protein was specifically bound to DRE elements (core sequence, A/GCCGAC) and activated the expression of the reporter HIS3 and LacZ. Transient expression experiment suggested that DvDREB2A protein was localized to the nucleus of onion epidermis cells. Quantitative real-time PCR (QRT-PCR) experiments showed that expression level of DvDREB2A was significantly affected by heat, low temperature, drought, abscisic acid (ABA) and high salinity treatments. These results indicated that the DvDREB2A gene is a new member of the DREB transcription factors, which may play an important role in providing tolerance to environmental stresses.

show abstract

An AP2/EREBP-type transcription-factor gene from rice is cold-inducible and encodes a nuclear-localized protein

Cited by 65 publications

References 36 publications

AP2-ERF Transcription Factors Mediate Nod Factor–Dependent MtENOD11Activation in Root Hairs via a Novelcis-Regulatory Motif

AP2-ERF Transcription Factors Mediate Nod Factor–Dependent MtENOD11Activation in Root Hairs via a Novelcis-Regulatory Motif

Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice

Molecular cloning, expression profiling and trans-activation property studies of a DREB2-like gene from chrysanthemum (Dendranthema vestitum)

Contact Info

Product

Resources

About